Pcsk1 (NM_013628) Mouse Tagged ORF Clone

CAT#: MG225451

Pcsk1 (GFP-tagged) - Mouse proprotein convertase subtilisin/kexin type 1 (Pcsk1), (10ug)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Pcsk1
• Synonyms: Nec-1; Nec1; PC1; PC3; Phpp-1; SPC3
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG225451 representing NM_013628
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCAAAGAGGTTGGACTCTGCAGTGTACTGCTTTCGCCTTCTTTTGCGTTTGGTGTGCACTAAACA
  GTGTAAAAGCAAAGAGGCAGTTTGTTAATGAATGGGCGGCGGAGATCCCCGGAGGGCAAGAAGCTGCCTC
  TGCCATCGCCGAAGAACTGGGGTATGACCTTTTGGGTCAGATTGGATCACTTGAAAATCACTATTTATTC
  AAACACAAAAGCCATCCTCGGAGGTCTCGAAGAAGCGCTCTTCATATCACTAAGAGGTTATCTGATGATG
  ATCGTGTGACGTGGGCTGAACAACAGTATGAAAAAGAGAGAAGTAAACGTTCAGTTCAAAAAGACTCAGC
  ATTGGATCTCTTCAATGATCCAATGTGGAATCAGCAGTGGTACTTGCAAGATACCAGAATGACTGCAGCT
  CTGCCCAAGCTGGACCTTCATGTAATACCTGTTTGGGAAAAGGGTATTACTGGCAAAGGAGTTGTTATTA
  CTGTACTGGATGATGGCTTGGAGTGGAATCACACAGACATTTATGCCAATTATGATCCAGAGGCTAGCTA
  TGATTTTAACGATAATGATCATGATCCATTTCCCCGATATGATCTCACAAATGAAAACAAACATGGAACA
  AGATGTGCAGGTGAAATTGCCATGCAAGCAAATAATCACAAGTGTGGGGTTGGAGTTGCATATAATTCCA
  AAGTTGGAGGCATAAGAATGCTGGATGGCATTGTAACTGATGCCATTGAGGCTAGTTCAATTGGATTCAA
  CCCTGGCCATGTGGATATTTACAGTGCAAGCTGGGGCCCTAATGATGATGGAAAAACTGTGGAGGGGCCT
  GGCAGACTAGCCCAGAAGGCATTTGAATATGGTGTCAAACAGGGGAGACAAGGGAAAGGCTCCATCTTTG
  TCTGGGCTTCAGGGAATGGGGGTCGTCAGGGAGATAACTGTGACTGTGATGGCTACACAGACAGCATTTA
  CACCATCTCTATCAGCAGTGCCTCCCAGCAAGGCCTGTCACCTTGGTATGCAGAGAAGTGTTCTTCCACA
  TTGGCTACCTCCTACAGCAGTGGTGATTACACAGACCAGCGAATAACAAGCGCTGACCTGCACAATGACT
  GCACAGAGACCCACACAGGCACCTCGGCTTCAGCACCCCTGGCTGCTGGTATCTTTGCTCTGGCCTTGGA
  GGCAAACCCAAATCTTACCTGGAGAGATATGCAGCATCTGGTTGTCTGGACCTCTGAGTACGACCCATTG
  GCCAGTAACCCAGGTTGGAAAAAGAATGGGGCAGGCTTGATGGTGAACAGCCGATTTGGATTTGGCTTGC
  TAAATGCCAAAGCTCTGGTGGATTTGGCTGATCCTAGGACCTGGAGAAATGTGCCTGAGAAGAAAGAATG
  TGTTGTAAAAGACAATAACTTTGAGCCTAGAGCCCTGAAAGCTAATGGAGAAGTAATTGTTGAAATCCCA
  ACAAGAGCTTGTGAAGGACAAGAAAATGCTATCAAGTCTCTGGAACATGTGCAATTTGAAGCAACAATTG
  AATATTCTCGTAGAGGAGACCTTCATGTCACACTCACTTCTGCTGTTGGAACCAGCACTGTACTGTTGGC
  TGAAAGGGAAAGAGATACATCCCCCAATGGCTTTAAGAATTGGGACTTCATGTCTGTTCATACATGGGGA
  GAGAATCCTGTAGGCACCTGGACATTGAAAATTACAGACATGTCTGGAAGAATGCAAAATGAAGGAAGGA
  TTGTGAACTGGAAGTTGATTTTGCATGGGACATCTTCTCAACCAGAGCACATGAAGCAGCCTCGTGTGTA
  CACATCCTACAATACAGTCCAGAATGACAGGAGAGGAGTGGAAAAGATGGTGAATGTTGTGGAGAAGCGG
  CCCACACAAAAGAGCCTGAATGGCAATCTCCTGGTACCCAAAAACTCCAGCAGCAGCAATGTGGAGGGTA
  GAAGGGATGAGCAGGTACAAGGAACTCCTTCAAAGGCCATGCTGCGACTCCTACAAAGTGCTTTTAGCAA
  GAATGCACTTTCAAAACAATCACCAAAGAAGTCTCCAAGTGCAAAGCTCAGCATCCCTTATGAAAGTTTC
  TATGAAGCCTTGGAAAAGCTCAACAAGCCCTCCAAGCTTGAAGGCTCTGAAGACAGTCTGTACAGTGACT
  ATGTTGATGTATTCTATAACACAAAACCTTATAAGCATAGAGATGACAGGCTGCTGCAAGCTCTCATGGA
  CATCCTAAATGAGGAGAAT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG225451 representing NM_013628
  Red=Cloning site Green=Tags(s)
  
  MEQRGWTLQCTAFAFFCVWCALNSVKAKRQFVNEWAAEIPGGQEAASAIAEELGYDLLGQIGSLENHYLF
  KHKSHPRRSRRSALHITKRLSDDDRVTWAEQQYEKERSKRSVQKDSALDLFNDPMWNQQWYLQDTRMTAA
  LPKLDLHVIPVWEKGITGKGVVITVLDDGLEWNHTDIYANYDPEASYDFNDNDHDPFPRYDLTNENKHGT
  RCAGEIAMQANNHKCGVGVAYNSKVGGIRMLDGIVTDAIEASSIGFNPGHVDIYSASWGPNDDGKTVEGP
  GRLAQKAFEYGVKQGRQGKGSIFVWASGNGGRQGDNCDCDGYTDSIYTISISSASQQGLSPWYAEKCSST
  LATSYSSGDYTDQRITSADLHNDCTETHTGTSASAPLAAGIFALALEANPNLTWRDMQHLVVWTSEYDPL
  ASNPGWKKNGAGLMVNSRFGFGLLNAKALVDLADPRTWRNVPEKKECVVKDNNFEPRALKANGEVIVEIP
  TRACEGQENAIKSLEHVQFEATIEYSRRGDLHVTLTSAVGTSTVLLAERERDTSPNGFKNWDFMSVHTWG
  ENPVGTWTLKITDMSGRMQNEGRIVNWKLILHGTSSQPEHMKQPRVYTSYNTVQNDRRGVEKMVNVVEKR
  PTQKSLNGNLLVPKNSSSSNVEGRRDEQVQGTPSKAMLRLLQSAFSKNALSKQSPKKSPSAKLSIPYESF
  YEALEKLNKPSKLEGSEDSLYSDYVDVFYNTKPYKHRDDRLLQALMDILNEEN
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_013628
• ORF Size: 2259 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_013628.2, NP_038656.1
• RefSeq Size: 2516
• RefSeq ORF: 2262
• Locus ID: 18548