Prkg1 (NM_011160) Mouse Tagged ORF Clone

CAT#: MG226311

Prkg1 (GFP-tagged) - Mouse protein kinase cGMP-dependent type I (Prkg1) transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Prkg1
• Synonyms: AW125416; CGKI; Gm19690; Prkg1b; Prkgr1b
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG226311 representing NM_011160
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCACCCTGCGGGATTTACAGTATGCGCTCCAGGAGAAGATCGAGGAGCTGAGGCAGCGGGATGCTC
  TCATCGATGAGCTGGAGCTGGAGTTGGATCAGAAGGACGAACTGATCCAGAAGCTGCAGAACGAACTGGA
  CAAGTATCGCTCGGTGATCCGGCCGGCCACCCAGCAGGCGCAGAAGCAGAGCGCCAGCACCTTGCAGGGT
  GAACCGCGCACCAAGCGCCAGGCGATCTCCGCGGAGCCCACCGCCTTCGACATCCAGGATCTCAGCCACG
  TGACCCTGCCCTTCTACCCCAAGAGCCCACAGTCGAAGGATCTCATAAAGGAGGCTATCCTTGACAATGA
  CTTCATGAAGAACTTGGAGCTGTCACAGATCCAGGAGATTGTGGACTGTATGTACCCCGTGGAATACGGC
  AAGGACAGCTGCATCATCAAGGAAGGCGATGTGGGGTCACTGGTGTACGTCATGGAAGATGGGAAGGTTG
  AAGTCACAAAAGAAGGCGTGAAGCTCTGCACCATGGGTCCTGGAAAAGTGTTCGGGGAGCTGGCTATACT
  TTACAACTGTACCCGGACAGCGACCGTCAAGACTCTTGTAAATGTGAAACTCTGGGCCATCGATCGACAA
  TGTTTTCAAACAATAATGATGAGGACAGGACTCATCAAGCATACCGAGTACATGGAATTTTTAAAAAGTG
  TTCCAACATTCCAGAGCCTTCCTGATGAAATCCTCAGCAAGCTGGCTGATGTCCTCGAAGAGACCCACTA
  TGAAAATGGAGAATATATCATCAGGCAGGGTGCAAGAGGAGACACCTTCTTCATCATCAGTAAAGGGCAG
  GTGAATGTTACTCGAGAAGACTCACCAAGTGAAGACCCAGTCTTCCTTAGAACTTTAGGGAAGGGAGATT
  GGTTTGGAGAGAAAGCGTTGCAGGGGGAGGATGTGAGAACAGCAAATGTTATTGCCGCAGAAGCTGTCAC
  CTGCCTTGTGATTGACAGAGACTCTTTCAAGCATTTGATTGGAGGACTGGACGATGTTTCTAACAAAGCA
  TATGAGGACGCAGAAGCAAAAGCAAAATATGAAGCCGAAGCTGCCTTCTTCGCCAACCTGAAGCTGTCTG
  ATTTCAACATCATTGACACCCTTGGAGTTGGAGGTTTCGGACGAGTAGAGCTGGTCCAGTTGAAAAGTGA
  AGAATCCAAAACATTTGCAATGAAGATCCTCAAGAAACGCCACATTGTGGACACCAGACAGCAGGAGCAC
  ATCCGCTCAGAGAAGCAGATCATGCAGGGGGCTCATTCTGACTTCATTGTGAGGCTGTACAGGACATTTA
  AAGACAGCAAATACTTGTATATGTTGATGGAAGCGTGCCTGGGTGGAGAGCTCTGGACTATTCTCAGGGA
  TAGGGGTTCGTTTGAAGACTCAACAACCAGGTTTTACACTGCATGTGTGGTAGAAGCATTCGCCTATCTG
  CATTCCAAAGGAATCATTTACAGGGACCTCAAGCCGGAGAATCTCATCCTAGATCATCGAGGCTATGCCA
  AACTGGTTGACTTTGGCTTTGCAAAGAAAATAGGATTTGGAAAGAAAACATGGACTTTTTGTGGGACTCC
  AGAATATGTAGCCCCAGAGATCATCCTGAACAAAGGCCATGACATTTCAGCTGACTACTGGTCACTAGGA
  ATTCTGATGTATGAGCTTCTGACTGGCAGCCCACCTTTCTCAGGCCCAGATCCAATGAAAACCTACAATA
  TCATACTGCGGGGGATTGACATGATAGAATTTCCAAAGAAGATTGCAAAAAATGCTGCTAATTTAATTAA
  AAAACTATGCAGGGACAACCCATCAGAGAGGTTAGGAAATTTGAAAAACGGAGTCAAAGACATTCAGAAA
  CACAAGTGGTTTGAGGGCTTTAATTGGGAAGGCTTAAGAAAAGGCACCTTGACACCTCCCATAATTCCAA
  GTGTTGCGTCACCCACAGACACAAGCAATTTTGACAGTTTCCCTGAGGACAGCGATGAGCCACCACCTGA
  TGACAACTCAGGCTGGGACATAGACTTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG226311 representing NM_011160
  Red=Cloning site Green=Tags(s)
  
  MGTLRDLQYALQEKIEELRQRDALIDELELELDQKDELIQKLQNELDKYRSVIRPATQQAQKQSASTLQG
  EPRTKRQAISAEPTAFDIQDLSHVTLPFYPKSPQSKDLIKEAILDNDFMKNLELSQIQEIVDCMYPVEYG
  KDSCIIKEGDVGSLVYVMEDGKVEVTKEGVKLCTMGPGKVFGELAILYNCTRTATVKTLVNVKLWAIDRQ
  CFQTIMMRTGLIKHTEYMEFLKSVPTFQSLPDEILSKLADVLEETHYENGEYIIRQGARGDTFFIISKGQ
  VNVTREDSPSEDPVFLRTLGKGDWFGEKALQGEDVRTANVIAAEAVTCLVIDRDSFKHLIGGLDDVSNKA
  YEDAEAKAKYEAEAAFFANLKLSDFNIIDTLGVGGFGRVELVQLKSEESKTFAMKILKKRHIVDTRQQEH
  IRSEKQIMQGAHSDFIVRLYRTFKDSKYLYMLMEACLGGELWTILRDRGSFEDSTTRFYTACVVEAFAYL
  HSKGIIYRDLKPENLILDHRGYAKLVDFGFAKKIGFGKKTWTFCGTPEYVAPEIILNKGHDISADYWSLG
  ILMYELLTGSPPFSGPDPMKTYNIILRGIDMIEFPKKIAKNAANLIKKLCRDNPSERLGNLKNGVKDIQK
  HKWFEGFNWEGLRKGTLTPPIIPSVASPTDTSNFDSFPEDSDEPPPDDNSGWDIDF
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_011160
• ORF Size: 2058 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_011160.3, NP_035290.1
• RefSeq Size: 6915
• RefSeq ORF: 2061
• Locus ID: 19091