Bub1 (NM_001113179) Mouse Tagged ORF Clone

CAT#: MG226889

Bub1 (GFP-tagged) - Mouse budding uninhibited by benzimidazoles 1 homolog (S. cerevisiae) (Bub1) transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Bub1
• Synonyms: AL022991; Bub1a; C80208; D2Xrf87
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG226889 representing NM_001113179
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACAACCTAGAAAATGTCTTTCGCATGTTTGAAGCCCATATGCAAAGCTACACGGGTAATGACCCAC
  TTGGAGAATGGGAAAGCTTTATAAAGTGGGTAGAAGAGAATTTTCCTGACAATAAAGAATACTTGATGAC
  ATTATTAGAACATTTAATGAAGGAATTTTTACATAAGAAGAACTACCACAATGATTCAAGATTCATCAAT
  TATTGCTTAAAATTTGCTGAGTACAACAGCGACCGTCATCAGTTTTTTGAGTTTCTGTACAACCAGGGAA
  TTGGAACCAAGTCATCATATATATACATGTCCTGGGCAGGGCATCTGGAAGCCCAGGGAGAGCTGCAGCA
  TGCCAGTGCTATTTTTCAGACAGGAATTCACAATGAGGCTGAACCTAAAGAACTACTACAGCAACAATAC
  AGGCTATTCCAAGCACGCCTTACTGGAATCCATTTGCCAGCTCAAGCTACAACCTCAGAACCTTTGCATA
  GTGCACAGATTTTAAACCAAGTTATGATGACAAACTCAAGTCCAGAAAAAAACTCAGCCTGTGTTCCTAA
  GAGTCAGGGTTCAGAATGTTCTGGTGTGGCATCTTCCACTTGTGATGAAAAGTCTAATATTAGGGAACAA
  AGGGTGATCATGATTTCCAAGTCAGAATGCTCTGTCAGCTCATCTGTGGCACCCAAGCCTGAGGCTCAGC
  AAGTTATGTACTGCAAGGAAAAGCTTATTCGTGGAGATTCAGAATTTTCTTTTGAAGAACTGAGAGCCCA
  GAAATATAATCAAAGGAAGAAGCATGAGCAGTGGGTTAGTGAAGACAGAAATTATATGAAAAGGAAAGAA
  GCAAATGCTTTTGAAGAGCAATTATTAAAACAGAAAATGGATGAACTTCACAAGAAATTGCATCAAGTGG
  TGGAATTGTCACACAAGGACCTTCCTGCTTCTGAGAACAGGCCTGATGTTAGTCTAGTATGTGTTGGACA
  AAATACTTGCTCCCAGCAGGAATTGAGGGGTCCAAGTCTTTCATCCATCAGTCATCAGACCTCAGAGAGT
  TCAGGAGAGAAACCACAGGAAGAACCTTCTGTTCCTCTTATGGTAAATGCTGTTAACAGCACTTTGCTGT
  TCCCAGCTGCCAACCTGCCAGCTCTTCCTGTTCCTGTAAGTGGCCAGTCATTGACAGACTCCAGATGTGT
  GAATCAAAGTGTTCATGAATTCATGCCACAGTGTGGACCAGAAACAAAAGAAGTGTGTGAAACAAATAAA
  GTTGCCAGCATTAATGATTTTCATACAACTCCAAACACATCATTGGGAATGGTTCAAGGAACACCATGCA
  AAGTGCAGCCATCACCAACTGTCCACACCAAGGAAGCATTAGGTTTCATCATGGACATGTTTCAGGCTCC
  AACACTTCCTGACATTTCTGATGATAAAGATGAATGGCCATCTCTGGACCAAAATGAAGATGCATTTGAA
  GCCCAGTTTCAAAAAAATGCAGTATCTTCGGGAGATTGGGGAGTTAAAAAAATTATGACTTTGTCATCTG
  CTTTTCCTATTTTTGAAGATGGAAACAAAGAAAATTATGGCTTACCACAGCCTAAAAATAAGCCCTTAGG
  AGCTAGGACCTTTGGAGAACGATCTCTCAGTAAATATTCCTCGAGATCAAATGAAATGCCTCACACTGAT
  GAGTTTATGGATGATTCAACAGTATGTGGTATTCGCTGCAACAAAACTCTAGCTCCCAGTCCTAAAAGTA
  TAGGAGACTTTACATCTGCTGCCCAACTTTCGTCTACACCATTCCACAAATTTCCAGCAGATTTAGTACA
  GATTCCAGAAGATAAAGAAAATGTGGTAGCCACACAGTATACACATATGGCTTTGGATTCTTGTAAAGAA
  AACATAGTGGACCTCTCAAAAGGCAGAAAGCTTGGGCCAATTCAAGAGAAAATTTCAGCATCTTTACCCT
  GTCCTAGTCAGCCTGCCACAGGTGGTTTGTTCACCCAGGAAGCAGTGTTCGGCCTTGAGGCTTTTAAATG
  CACAGGCATTGACCATGCGACAGTGGAAGACCTATCCGATGCCAATGCTGGGCTCCAAGTTGAATGCGTG
  CAGACACTTGGAAATGTCAATGCTCCAAGCTTTACTGTTGAGAACCCATGGGATGATGAATTGATTCTTA
  AACTTCTCTCTGGACTTTCTAAGCCAGTTACTTCCTATTCAAATACTTTTGAGTGGCAGAGTAAACTTCC
  AGCCATCAAGACCAAGACAGAATATCAATTGGGTTCTTTGCTGGTCTATGTGAATCACCTTCTTGGAGAA
  GGAGCCTTTGCTCAAGTCTTTGAAGCTATTCATGGAGATGTGAGAAATGCCAAAAGTGAACAGAAATGCA
  TTTTGAAGGTGCAGAGACCTGCCAACTCCTGGGAATTCTACATTGGGATGCAGCTGATGGAAAGACTAAA
  GCCAGAAGTACATCACATGTTCATCAAGTTTTATTCTGCTCATTTATTCAAGAACGGCAGCATATTAGTA
  GGGGAACTCTACAGCTATGGGACGTTACTAAATGTCATTAACCTCTATAAAAATACCTCTGAAAAAGTGA
  TGCCCCAGGCTCTTGTCCTCACTTTCGCTATCAGAATGCTTTACATGGTTGAACAAGTCCACAGCTGCGA
  AATCATTCATGGAGACATTAAGCCAGATAACTTCATACTAGGACACAGATTTTTGGAACAGGCTGATGAA
  GACTTAGCTACCGGCTTGGCATTGATTGACCTGGGTCAGAGTATAGATATGAAACTTTTCCCTAAAGGAA
  CTGTATTTACAGGAAAATGTGAAACATCTGGTTTTCAGTGTCCTGAGATGCTCAGTAACAAGCCATGGAA
  CTACCAGATTGATTACTTTGGAGTTGCTGCAACAATATACTGTATGCTCTTTGGCTCTTACATGAAAGTA
  AAAAATGAAGGAGGAGTCTGGAAACCTGAAGGTCTTTTTAGAAGGCTTCCTCATTTGGATATGTGGGAGG
  AATTTTTTCACATCATGTTGAATATACCGGATTGTCATAATCTTCCATCTTTGGATTTTCTGAGACAGAA
  TATGAAGAAATTACTTGAACAACAGTATTCCAACAAGATTAAGACCTTGCGTAATAGGCTAATTGTGATG
  CTTTCAGAATATAAGCGTTCAAGAAAA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG226889 representing NM_001113179
  Red=Cloning site Green=Tags(s)
  
  MDNLENVFRMFEAHMQSYTGNDPLGEWESFIKWVEENFPDNKEYLMTLLEHLMKEFLHKKNYHNDSRFIN
  YCLKFAEYNSDRHQFFEFLYNQGIGTKSSYIYMSWAGHLEAQGELQHASAIFQTGIHNEAEPKELLQQQY
  RLFQARLTGIHLPAQATTSEPLHSAQILNQVMMTNSSPEKNSACVPKSQGSECSGVASSTCDEKSNIREQ
  RVIMISKSECSVSSSVAPKPEAQQVMYCKEKLIRGDSEFSFEELRAQKYNQRKKHEQWVSEDRNYMKRKE
  ANAFEEQLLKQKMDELHKKLHQVVELSHKDLPASENRPDVSLVCVGQNTCSQQELRGPSLSSISHQTSES
  SGEKPQEEPSVPLMVNAVNSTLLFPAANLPALPVPVSGQSLTDSRCVNQSVHEFMPQCGPETKEVCETNK
  VASINDFHTTPNTSLGMVQGTPCKVQPSPTVHTKEALGFIMDMFQAPTLPDISDDKDEWPSLDQNEDAFE
  AQFQKNAVSSGDWGVKKIMTLSSAFPIFEDGNKENYGLPQPKNKPLGARTFGERSLSKYSSRSNEMPHTD
  EFMDDSTVCGIRCNKTLAPSPKSIGDFTSAAQLSSTPFHKFPADLVQIPEDKENVVATQYTHMALDSCKE
  NIVDLSKGRKLGPIQEKISASLPCPSQPATGGLFTQEAVFGLEAFKCTGIDHATVEDLSDANAGLQVECV
  QTLGNVNAPSFTVENPWDDELILKLLSGLSKPVTSYSNTFEWQSKLPAIKTKTEYQLGSLLVYVNHLLGE
  GAFAQVFEAIHGDVRNAKSEQKCILKVQRPANSWEFYIGMQLMERLKPEVHHMFIKFYSAHLFKNGSILV
  GELYSYGTLLNVINLYKNTSEKVMPQALVLTFAIRMLYMVEQVHSCEIIHGDIKPDNFILGHRFLEQADE
  DLATGLALIDLGQSIDMKLFPKGTVFTGKCETSGFQCPEMLSNKPWNYQIDYFGVAATIYCMLFGSYMKV
  KNEGGVWKPEGLFRRLPHLDMWEEFFHIMLNIPDCHNLPSLDFLRQNMKKLLEQQYSNKIKTLRNRLIVM
  LSEYKRSRK
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001113179
• ORF Size: 3177 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001113179.1, NP_001106650.1
• RefSeq Size: 4337
• RefSeq ORF: 3180
• Locus ID: 12235