Gimap4 (BC005577) Mouse Tagged ORF Clone

CAT#: MR202489

Gimap4 (Myc-DDK-tagged) - Mouse GTPase, IMAP family member 4 (cDNA clone MGC:11734 IMAGE:3968418)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Gimap4
• Synonyms: MGC11734, IMAP4, mIAN1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR202489 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAGTCCAGTGCGGTGGTGCGGGGTTCATCCCAGAAAGTTCAAGGAGCAGCCATGAGCTTGGAAACC
  AAGATCAAGGAATTCCCCAACTGAGAATTGTCTTACTTGGAAAAACTGGAGCAGGAAAGAGTTCAACAGG
  GAACAGTATCCTTGGGGAAAAAGTGTTTAATTCTGGCATTTGTGCAAAATCCATCACCAAGGTCTGTGAA
  AAAAGGGTGAGCACCTGGGATGGGAAAGAGCTTGTTGTCGTGGATACACCTGGTATTTTTGACACTGAGG
  TACCAGATGCTGACACACAAAGGGAGATCACTCGCTATGTTGCCCTGACCTCTCCAGGGCCTCATGCTCT
  GCTCCTGGTAGTTCCACTGGGGCGTTATACTGTGGAAGAACACAAGGCTACACAGAAAATTCTGGACATG
  TTTGGAAAACAGGCTAGAAGATTCATGATTCTCTTGCTCACCAGGAAGGATGACTTAGAAGACACTGATA
  TCCATGAGTACTTAGAGAAGGCTCCTAAATTCTTTCAAGAGGTGATGCATGAGTTCCAGAATCGCTACTG
  TTTGTTCAACAACAGAGCCTCAGGCGCTGAAAAGGAAGAGCAGAAGATGCAATTGTTGACCTTGGTCCAG
  AGCATGTTTCTGTCTTCCAGGATGAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR202489 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MEVQCGGAGFIPESSRSSHELGNQDQGIPQLRIVLLGKTGAGKSSTGNSILGEKVFNSGICAKSITKVCE
  KRVSTWDGKELVVVDTPGIFDTEVPDADTQREITRYVALTSPGPHALLLVVPLGRYTVEEHKATQKILDM
  FGKQARRFMILLLTRKDDLEDTDIHEYLEKAPKFFQEVMHEFQNRYCLFNNRASGAEKEEQKMQLLTLVQ
  SMFLSSRMK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC005577
• ORF Size: 657 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC005577, AAH05577
• RefSeq Size: 1364 bp
• RefSeq ORF: 659 bp
• Locus ID: 107526
• MW: 24.6 kDa
• Gene Summary: This gene encodes a protein belonging to the GTP-binding superfamily and to the immuno-associated nucleotide (IAN) subfamily of nucleotide-binding proteins. This gene exists within a cluster of other related genes located on mouse chromosome 6. This family member encodes a lymphoid signaling protein that functions to accelerate programmed T-cell death, which appears to correlate with the phosphorylation status of the protein. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2011]