Casp6 (NM_009811) Mouse Tagged ORF Clone

CAT#: MR203708

Casp6 (Myc-DDK-tagged) - Mouse caspase 6 (Casp6)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Casp6
• Synonyms: CASP-6; Mch2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR203708 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACAGAAACCGATGGCTTCTACAAAAGTAGGGAAGTGTTCGATCCAGCCGAGCAGTACAAGATGGACC
  ACAAGAGGAGAGGAGTTGCCCTCATCTTCAATCACGAGAGGTTCTTTTGGCACCTGACCCTCCCAGAGAG
  GCGGGGCACCAACGCAGACAGAGACAACCTGACTCGCAGGTTTTCAGATCTAGGATTTGAAGTGAAATGC
  TTTAACGACCTCAGAGCAGAAGAACTCCTGCTCAAAATTCACGAGGTGTCGACTTCAAGCCACATAGATG
  CCGATTGCTTCATCTGTGTCTTCCTGAGCCACGGGGAAGGCAACCACGTTTACGCATACGACGCCAAAAT
  TGAAATTCAGACGTTGACTGGCTTGTTCAAAGGAGACAAATGTCAGAGCCTGGTTGGAAAACCCAAGATA
  TTTATCATCCAGGCCTGTCGGGGCAGCCAGCATGACGTACCTGTGGTTCCCCTGGACGTGGTGGATCACC
  AGACAGACAAGCTGGACAACGTGACCCAGGTGGATGCTGCGTCTGTGTACACGCTGCCGGCAGGGGCAGA
  CTTCCTCATGTGCTACTCTGTCGCAGAAGGGTATTACTCTCACCGAGAAACTGTGAATGGCTCCTGGTAC
  ATTCAGGATTTGTGTGAGATGCTGGCGAGGTACGGCAGTTCCCTGGAGTTCACGGAGCTGCTCACGCTGG
  TGAACAGAAAGGTCTCTCAGCGCCGCGTGGACTTCTGCAAAGACCCGGATGCAATCGGCAAGAAGCAGGT
  GCCCTGCTTCGCCTCAATGCTGACCAAAAAGCTGCATTTCTGTCCCAAACCTAGCAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR203708 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MTETDGFYKSREVFDPAEQYKMDHKRRGVALIFNHERFFWHLTLPERRGTNADRDNLTRRFSDLGFEVKC
  FNDLRAEELLLKIHEVSTSSHIDADCFICVFLSHGEGNHVYAYDAKIEIQTLTGLFKGDKCQSLVGKPKI
  FIIQACRGSQHDVPVVPLDVVDHQTDKLDNVTQVDAASVYTLPAGADFLMCYSVAEGYYSHRETVNGSWY
  IQDLCEMLARYGSSLEFTELLTLVNRKVSQRRVDFCKDPDAIGKKQVPCFASMLTKKLHFCPKPSK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_009811
• ORF Size: 831 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_009811.1, NM_009811.2, NM_009811.3, NM_009811.4, NP_033941.2
• RefSeq Size: 1455 bp
• RefSeq ORF: 831 bp
• Locus ID: 12368
• MW: 31.6 kDa
• Gene Summary: This gene encodes a member of the cysteine proteases that plays important roles in regulating apoptosis and neurodegeneration. The encoded protein is involved in the transmission of pain and axonal degeneration. Genetic deletion of this gene in mice results in the delay of axon pruning and protects from axon degeneration. [provided by RefSeq, Apr 2015]