Ckb (NM_021273) Mouse Tagged ORF Clone

CAT#: MR205953

Ckb (Myc-DDK-tagged) - Mouse creatine kinase, brain (Ckb)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ckb
• Synonyms: B-CK; Bck; Ck-3; Ck3; Ckbb; CPK-B
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR205953 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCCTTCTCCAACAGCCATAATACGCAGAAGCTGCGCTTCCCGGCCGAGGATGAGTTCCCTGATCTGA
  GCAGCCACAACAACCATATGGCCAAGGTGCTGACCCCGGAGCTGTACGCCGAGCTCCGTGCCAAGTGCAC
  GCCGAGCGGCTTTACTTTGGACGACGCCATTCAGACTGGCGTAGACAATCCGGGCCACCCGTACATCATG
  ACTGTGGGTGCAGTGGCGGGCGACGAGGAGAGTTACGACGTATTCAAGGACCTCTTCGACCCCATTATTG
  AGGAGCGGCACGGCGGCTACCAGCCCAGTGATGAGCACAAGACCGACCTCAACCCAGACAACCTGCAGGG
  TGGCGATGACCTGGACCCCAACTACGTGCTGAGCTCGCGAGTGCGCACAGGCCGCAGCATCCGCGGCTTC
  TGTCTCCCCCCGCACTGCAGCCGCGGGGAGCGCCGCGCCATCGAGAAGCTGGCAGTAGAAGCTCTGTCCA
  GCCTAGATGGCGACCTGTCTGGCAGGTACTACGCGCTCAAGAGCATGACTGAGGCGGAGCAGCAGCAGCT
  CATTGACGACCACTTCCTCTTCGATAAGCCTGTGTCGCCTCTGCTGCTGGCCTCCGGCATGGCCCGCGAC
  TGGCCGGATGCTCGTGGCATATGGCACAATGACAATAAGACTTTCCTGGTGTGGATTAACGAGGAGGACC
  ACCTGCGAGTCATCTCCATGCAGAAGGGGGGCAACATGAAGGAAGTGTTCACCCGATTCTGCACCGGCCT
  CACTCAGATCGAAACTCTCTTCAAGTCCAAGAACTATGAGTTCATGTGGAATCCTCACCTGGGCTACATC
  CTCACATGCCCATCCAACCTGGGCACCGGACTGCGGGCAGGTGTGCACATCAAGCTGCCCCACCTGGGGA
  AGCACGAGAAGTTCTCGGAGGTGCTCAAGCGGCTGCGGCTTCAGAAGCGAGGCACAGGTGGCGTGGACAC
  CGCTGCTGTCGGTGGGGTGTTTGATGTCTCCAACGCTGACCGCCTGGGCTTCTCGGAGGTGGAACTGGTG
  CAGATGGTGGTGGACGGAGTGAAACTACTCATTGAGATGGAGCAACGGCTCGAGCAGGGTCAGGCAATCG
  ATGACCTCATGCCGGCCCAGAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR205953 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MPFSNSHNTQKLRFPAEDEFPDLSSHNNHMAKVLTPELYAELRAKCTPSGFTLDDAIQTGVDNPGHPYIM
  TVGAVAGDEESYDVFKDLFDPIIEERHGGYQPSDEHKTDLNPDNLQGGDDLDPNYVLSSRVRTGRSIRGF
  CLPPHCSRGERRAIEKLAVEALSSLDGDLSGRYYALKSMTEAEQQQLIDDHFLFDKPVSPLLLASGMARD
  WPDARGIWHNDNKTFLVWINEEDHLRVISMQKGGNMKEVFTRFCTGLTQIETLFKSKNYEFMWNPHLGYI
  LTCPSNLGTGLRAGVHIKLPHLGKHEKFSEVLKRLRLQKRGTGGVDTAAVGGVFDVSNADRLGFSEVELV
  QMVVDGVKLLIEMEQRLEQGQAIDDLMPAQK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_021273
• ORF Size: 1146 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_021273.1, NM_021273.2, NM_021273.3, NM_021273.4, NP_067248.1
• RefSeq Size: 1478 bp
• RefSeq ORF: 1146 bp
• Locus ID: 12709
• MW: 42.7 kDa
• Gene Summary: Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa. [UniProtKB/Swiss-Prot Function]