Arrb2 (NM_145429) Mouse Tagged ORF Clone

CAT#: MR206444

Arrb2 (Myc-DDK-tagged) - Mouse arrestin, beta 2 (Arrb2)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Arrb2
• Synonyms: AI326910; Arr3; AW122872
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR206444 representing NM_145429
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGAGAAAAACCCGGGACCAGGGTCTTCAAGAAGTCCAGCCCTAACTGCAAGCTCACCGTGTACTTGG
  GCAAGCGCGACTTTGTAGATCACCTGGACAAAGTGGACCCTGTGGATGGCGTGGTGCTTGTGGATCCTGA
  CTACTTGAAGGACCGGAAAGTGTTCGTGACCCTCACCTGTGCCTTCCGCTATGGCCGAGAAGACCTGGAT
  GTACTGGGCCTGTCTTTCCGCAAAGATCTGTTCATCGCCACCTACCAGGCCTTCCCCCCCATGCCCAACC
  CACCTCGGCCCCCCACCCGCCTACAGGACCGACTGCTGAAGAAGTTGGGCCAACACGCCCACCCCTTCTT
  TTTCACGATACCCCAAAATTTGCCTTGTTCCGTCACACTGCAGCCAGGACCAGAGGACACAGGGAAGGCT
  TGTGGAGTAGACTTTGAGATTCGAGCTTTCTGTGCCAAATCAATAGAAGAAAAAAGTCACAAAAGGAACT
  CTGTGCGGCTTATCATCAGAAAGGTACAGTTTGCTCCTGAGACACCCGGTCCCCAGCCCTCAGCTGAAAC
  CACACGCCACTTCCTCATGTCTGACCGGAGGTCCCTCCACCTAGAGGCTTCCCTGGACAAAGAGCTGTAC
  TACCACGGGGAGCCCCTTAATGTCAACGTCCATGTCACCAACAATTCTGCCAAGACCGTCAAGAAGATCA
  GAGTGTCTGTGAGACAGTACGCCGACATTTGCCTCTTCAGCACCGCGCAGTACAAGTGTCCCGTGGCTCA
  GCTAGAACAAGATGACCAGGTGTCTCCTAGTTCCACGTTCTGCAAGGTGTACACCATCACCCCGCTGCTC
  AGTGACAACCGAGAGAAGCGTGGCCTTGCCTTGGATGGGCAGCTCAAACATGAAGACACCAACCTGGCTT
  CCAGCACCATTGTGAAGGAGGGAGCCAACAAGGAGGTGCTGGGGATCCTGGTATCCTACAGGGTCAAGGT
  GAAGCTGGTGGTGTCTCGAGGCGGAGATGTCTCCGTGGAGCTACCCTTCGTCCTAATGCACCCCAAGCCC
  CATGACCACATCACCCTTCCCAGACCCCAGTCAGCCCCCCGGGAAACAGACGTGCCTGTGGATACCAACC
  TCATCGAATTCGATACCAACTATGCCACAGACGATGACATCGTGTTTGAGGACTTTGCCCGGCTCCGGCT
  GAAGGGAATGAAGGATGATGACTGTGATGACCAGTTCTGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR206444 representing NM_145429
  Red=Cloning site Green=Tags(s)
  
  MGEKPGTRVFKKSSPNCKLTVYLGKRDFVDHLDKVDPVDGVVLVDPDYLKDRKVFVTLTCAFRYGREDLD
  VLGLSFRKDLFIATYQAFPPMPNPPRPPTRLQDRLLKKLGQHAHPFFFTIPQNLPCSVTLQPGPEDTGKA
  CGVDFEIRAFCAKSIEEKSHKRNSVRLIIRKVQFAPETPGPQPSAETTRHFLMSDRRSLHLEASLDKELY
  YHGEPLNVNVHVTNNSAKTVKKIRVSVRQYADICLFSTAQYKCPVAQLEQDDQVSPSSTFCKVYTITPLL
  SDNREKRGLALDGQLKHEDTNLASSTIVKEGANKEVLGILVSYRVKVKLVVSRGGDVSVELPFVLMHPKP
  HDHITLPRPQSAPRETDVPVDTNLIEFDTNYATDDDIVFEDFARLRLKGMKDDDCDDQFC
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_145429
• ORF Size: 1230 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_145429.1, NM_145429.2, NM_145429.3, NM_145429.4, NM_145429.5, NP_663404.1
• RefSeq Size: 1729 bp
• RefSeq ORF: 1233 bp
• Locus ID: 216869
• MW: 46.8 kDa