Aup1 (BC016485) Mouse Tagged ORF Clone

CAT#: MR206456

Aup1 (Myc-DDK-tagged) - Mouse ancient ubiquitous protein (cDNA clone MGC:11483 IMAGE:3962982)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Aup1
• Synonyms: AA589454
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR206456 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAACCTCCCCCAGCTCCGGGGCCGGAGCGGCTCTTTGACTCGCACCGGCTCCCGAGTGACGGCTTCC
  TGCTGCTCGCGCTACTGCTCTACGCGCCGGTCGGCCTCTGCCTCCTGGTCCTGCGACTCTTCCTCGGGCT
  CCACGTCTTCCTGGTCAGCTGCGCTCTGCCCGACAGCGTCCTCCGCAGGTTCGTGGTACGAACCATGTGT
  GCGGTCCTGGGGCTCGTGGCCCGGCAGGAGGACTCCGGACTCCGCGATCACCGCGTCAGGGTCCTTATTT
  CCAACCACGTAACACCTTTTGACCACAACATAGTCAACCTCCTCACCACCTGTAGCACCCCTCTACTCAA
  TAGTCCCCCCAGCTTTGTGTGTTGGTCTCGGGGCTTCATGGAGATGGATAGGCGGGTGGAGTTGGTGGAG
  TCACTCAAGAAATTCTGTGCTTCCACGAGGCTTCCGCCCACACCTTTGCTGCTCTTCCCCGAGGAAGAGG
  CCACCAATGGCCGAGAAGGGCTGCTGCGTTTCAGTTCGTGGCCATTTTCTATTCAGGACGTGGTACAACC
  TCTTACCCTGCAAGTTCAGAGACCCCTGGTCTCTGTGACGGTGTCAGATGCCTCCTGGGTCTCAGAACTG
  CTGTGGTCCCTTTTTGTTCCTTTCACGGTGTATCAAGTAAGGTGGCTTCATCCCATTCGTCGACAGCTAG
  GGGAAGAGAGTGAGGAGTTTGCGCTCCGTGTACAACAGCTGGTGGCCAAAGAATTGGGCCAGATAGGGAC
  ACGGCTCACTCCAGCAGACAAAGCAGAACACATGAAGCGACAAAGACACCCCAGATTACGCCCCCAGTCA
  GTGCAGTCTTCTTTTCCTTCTCCTCCCAGCCCTTCTTCTGATGTGCAGCTGACCACTCTCGCTCACAGAG
  TCAAGGAGGTTCTGCCCCATGTGCCATTGAATGTCATCCAGAGAGACCTGGCCAGGACTGGGTGTGTAGA
  CTTGACCATCACAAACCTGCTCGAGGGGGCTGTGGCTTTCATGCCTGAAGATGTCACTGAGGGATCTCAG
  TCCCCGCCTGCACCCTCTGCCCCAAAGTTCCCCAGCTCGGGCCTGGCGACCCCTCAGCCCACAGCCCTAA
  CGTTTGCCAAGTCTTCCTGGGCCCGTCAGGAGAGCCTGCAGGAGCGCAAGCAGGCACTGTATGAATATGC
  AAGAAGGAGATTCAGAGAGAGACAGGCCCAGGAGGCTGAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR206456 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MEPPPAPGPERLFDSHRLPSDGFLLLALLLYAPVGLCLLVLRLFLGLHVFLVSCALPDSVLRRFVVRTMC
  AVLGLVARQEDSGLRDHRVRVLISNHVTPFDHNIVNLLTTCSTPLLNSPPSFVCWSRGFMEMDRRVELVE
  SLKKFCASTRLPPTPLLLFPEEEATNGREGLLRFSSWPFSIQDVVQPLTLQVQRPLVSVTVSDASWVSEL
  LWSLFVPFTVYQVRWLHPIRRQLGEESEEFALRVQQLVAKELGQIGTRLTPADKAEHMKRQRHPRLRPQS
  VQSSFPSPPSPSSDVQLTTLAHRVKEVLPHVPLNVIQRDLARTGCVDLTITNLLEGAVAFMPEDVTEGSQ
  SPPAPSAPKFPSSGLATPQPTALTFAKSSWARQESLQERKQALYEYARRRFRERQAQEAE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC016485
• ORF Size: 1230 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: BC016485, AAH16485
• RefSeq Size: 1454
• RefSeq ORF: 1232
• Locus ID: 11993
• MW: 46.1 kDa
• Gene Summary: The protein encoded by this gene contains several conserved domains including a hydrophobic domain, an acetyltransferase domain, a ubiquitin binding domain, and a domain required for recruitment of ubiquitin-conjugating enzyme E2 G2 (Ube2g2). In humans, this protein localizes to the endoplasmic reticulum and to lipid droplets. This protein is thought to be involved both in the degradation of misfolded proteins from the endoplasmic reticulum and in the storage of neutral lipids. Reduced expression of the human ortholog of this gene strongly reduces lipid droplet clustering in the cell, and causes stabilization of misfolded proteins. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2014]