Frmd5 (BC057549) Mouse Tagged ORF Clone

CAT#: MR206885

Frmd5 (Myc-DDK-tagged) - Mouse FERM domain containing 5 (cDNA clone MGC:66559 IMAGE:6416914)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Frmd5
• Synonyms: 5830442F04
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR206885 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTGCTTCCGCGTGAAGTTTTACCCTGCCGACCCCGCTGCCCTGAAAGAGGAAATAACCAGGTACTTAG
  TCTTCCTTCAGATCAAAAGGGATCTCTACCACGGCCGTCTTCTCTGTAAAACATCGGATGCCGCCTTGTT
  AGCGGCCTATATCCTTCAAGCGGAGATTGGGGATTATGACCCGGGAAAGCACCCTGAAGGCTACAGCTCC
  AAGTTCCAATTTTTCCCGAAACATTCAGAGAAACTGGAAAAGAAAATTGCTGAGATTCACAAGACCGAAC
  TCAGTGGTCAAACACCAGCAACATCAGAGCTGAATTTCTTAAGAAAGGCCCAGACGTTAGAGACCTACGG
  AGTGGACCCTCACCCATGTAAGGATGTGTCGGGAAATGCTGCGTTTCTGGCCTTCACTCCTTTCGGGTTT
  GTTGTTCTTCAAGGAAACAAGAGGGTCCACTTCATTAAATGGAATGAGGTGACCAAGCTGAAATTTGAAG
  GAAAGACTTTCTATTTATATGTAAGTCAGAAAGAGGAAAAAAAGATTATTCTCACATATTTTGCCCCAAC
  TCCAGAAGCCTGCAAGCACCTCTGGAAATGTGGAATTGAGAACCAAGCCTTCTACAAGCTGGAAAAGTCA
  AGCCAAGTCCGCACAGTGTCCAGCAGCAATTTGTTCTTTAAGGGGAGCCGGTTCCGATACAGTGGCCGGG
  TCGCAAAGGAAGTAATGGAGTCCAGTGCCAAGATCAAACGGGAGCCGCCAGAAATACACAGAGCAGGGAT
  GGTCCCTAGCCGCAGCTGTCCTTCTATAACCCATGGCCCACGACTGAGCAGCGTCCCCAGGACACGAAGA
  AGAGCTGTGCACATCTCTATCATGGAAGGCCTTGAGTCGCTACGAGACAGTGCCCATTCCACCCCAGTGC
  GGTCCTCTTCCCATGGGGACACCTTCCTGCCTCACGTGAGAAGCAGCCGGGCAGACAGCAATGAACGTGT
  CGCTGTGATAGCGGATGAGGCCTACAGCCCCGCAGACAGTGTGCTGCCCACCCCTGTAGCCGAGCACAGC
  CTGGAGCTGATGCTGCTCTCCCGGCAGATCAACGGGGCCACGTGCAGCATTGAGGAGGAGAAGGAGTCGG
  AGGCCAGCACCCCGACTGCGACAGAGGCGGAGGCCCTGGGAGGAGAGCTGAGGGCCCTGTGTCAGGGGCA
  CGGCGGGTCAGAGCAAGAACAGATGCAAGAGAGAGACAGATCTGAGGAAGGCATCGAGAAGGAGCAGCGG
  CCTTGGAAGGGACCAGGAAGCACTAAGCAGGACCTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR206885 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MCFRVKFYPADPAALKEEITRYLVFLQIKRDLYHGRLLCKTSDAALLAAYILQAEIGDYDPGKHPEGYSS
  KFQFFPKHSEKLEKKIAEIHKTELSGQTPATSELNFLRKAQTLETYGVDPHPCKDVSGNAAFLAFTPFGF
  VVLQGNKRVHFIKWNEVTKLKFEGKTFYLYVSQKEEKKIILTYFAPTPEACKHLWKCGIENQAFYKLEKS
  SQVRTVSSSNLFFKGSRFRYSGRVAKEVMESSAKIKREPPEIHRAGMVPSRSCPSITHGPRLSSVPRTRR
  RAVHISIMEGLESLRDSAHSTPVRSSSHGDTFLPHVRSSRADSNERVAVIADEAYSPADSVLPTPVAEHS
  LELMLLSRQINGATCSIEEEKESEASTPTATEAEALGGELRALCQGHGGSEQEQMQERDRSEEGIEKEQR
  PWKGPGSTKQDL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC057549
• ORF Size: 1296 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC057549, AAH57549
• RefSeq Size: 3370 bp
• RefSeq ORF: 1298 bp
• Locus ID: 228564
• MW: 48.5 kDa