Coro1a (NM_009898) Mouse Tagged ORF Clone

CAT#: MR207361

Coro1a (Myc-DDK-tagged) - Mouse coronin, actin binding protein 1A (Coro1a)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Coro1a
• Synonyms: Clabp; Lmb3; p57; TACO
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR207361 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCCGGCAGGTGGTTCGCTCCAGCAAATTCCGCCACGTGTTTGGACAGCCAGCCAAGGCTGACCAGT
  GCTATGAGGATGTGCGCGTCTCACAAACCACTTGGGACAGTGGCTTCTGCGCTGTCAACCCCAAGTTCAT
  GGCTCTGATCTGTGAGGCCAGTGGGGGAGGGGCCTTCCTGGTGCTACCCCTAGGCAAGACTGGACGAGTA
  GACAAGAACGTGCCCCTGGTCTGCGGCCACACTGCCCCTGTGCTAGACATCGCCTGGTGTCCACACAATG
  ACAATGTCATTGCCAGTGGCTCTGAGGACTGCACAGTTATGGTGTGGGAGATCCCGGATGGGGGCCTGGT
  GCTGCCCTTGCGGGAGCCTGTCGTCACCTTGGAGGGCCACACCAAGAGGGTTGGCATCGTGGCCTGGCAC
  CCCACAGCCCAGAATGTGCTTCTCAGCGCAGGTTGTGACAATGTGATCCTGGTGTGGGACGTGGGCACTG
  GGGCAGCTGTGCTGACGCTGGGCCCGGATGTGCACCCGGACACAATCTACAGCGTGGACTGGAGCCGGGA
  CGGTGCTCTCATCTGCACCTCCTGCCGTGACAAGCGAGTTCGTGTCATCGAGCCTCGCAAAGGCACTGTT
  GTGGCTGAAAAGGACCGTCCCCATGAGGGGACTCGGCCAGTGCACGCTGTGTTTGTGTCAGAAGGGAAGA
  TTCTAACCACGGGTTTCAGTCGCATGAGTGAGCGGCAGGTGGCGCTGTGGGACACAAAGCACCTGGAGGA
  GCCACTGTCCCTGCAGGAGCTGGACACAAGCAGCGGAGTCCTGCTACCTTTTTTTGATCCTGATACCAAC
  ATTGTCTACCTCTGTGGCAAGGGTGACAGCTCTATCCGGTATTTTGAGATCACTTCTGAGGCTCCGTTCC
  TGCATTATCTTTCCATGTTCAGTTCCAAGGAGTCTCAGCGTGGTATGGGCTACATGCCCAAACGTGGCCT
  GGAAGTGAATAAGTGTGAGATTGCCAGATTCTATAAGCTTCATGAGCGGAAGTGTGAGCCCATTGCCATG
  ACAGTGCCTAGAAAGTCGGACCTGTTCCAGGAGGACCTGTATCCGCCCACTGCAGGACCCGACCCTGCCC
  TCACAGCTGAGGAATGGCTGGGTGGTCGGGATGCTGGCCCCCTCCTCATTTCCCTCAAGGATGGCTACGT
  GCCCCCAAAGAGCAGGGAGCTGAGGGTCAACAGGGGCCTGGACAGCGCTCGCAGAAGAGCTACACCAGAG
  CCCAGCGGCACGCCCAGCTCGGATACCGTGTCAAGGCTGGAGGAGGACGTGAGGAATCTGAACGCCATAG
  TGCAAAAGCTACAAGAGCGCCTGGACAGGCTGGAGGAGACTGTTCAGGCCAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR207361 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSRQVVRSSKFRHVFGQPAKADQCYEDVRVSQTTWDSGFCAVNPKFMALICEASGGGAFLVLPLGKTGRV
  DKNVPLVCGHTAPVLDIAWCPHNDNVIASGSEDCTVMVWEIPDGGLVLPLREPVVTLEGHTKRVGIVAWH
  PTAQNVLLSAGCDNVILVWDVGTGAAVLTLGPDVHPDTIYSVDWSRDGALICTSCRDKRVRVIEPRKGTV
  VAEKDRPHEGTRPVHAVFVSEGKILTTGFSRMSERQVALWDTKHLEEPLSLQELDTSSGVLLPFFDPDTN
  IVYLCGKGDSSIRYFEITSEAPFLHYLSMFSSKESQRGMGYMPKRGLEVNKCEIARFYKLHERKCEPIAM
  TVPRKSDLFQEDLYPPTAGPDPALTAEEWLGGRDAGPLLISLKDGYVPPKSRELRVNRGLDSARRRATPE
  PSGTPSSDTVSRLEEDVRNLNAIVQKLQERLDRLEETVQAK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_009898
• ORF Size: 1386 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_009898.1, NM_009898.2, NM_009898.3, NP_034028.1
• RefSeq Size: 1686 bp
• RefSeq ORF: 1386 bp
• Locus ID: 12721
• MW: 51 kDa
• Gene Summary: This gene encodes a member of the WD repeat coronin family. The encoded protein may bind actin and interact with microtubules. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2014]