Abcd4 (BC050102) Mouse Tagged ORF Clone

CAT#: MR208310

Abcd4 (Myc-DDK-tagged) - Mouse ATP-binding cassette, sub-family D (ALD), member 4 (cDNA clone MGC:60642 IMAGE:30012556)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Abcd4
• Synonyms: P69r, P70R
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR208310 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACGCTCTTGTGCGTGACCCTCCTGGAGCAACTGGTGATCTACCAAGTTGGCTTGATCCCCAGTCAGT
  ATTATGGAGTCTTGGGAAACAAAGACCTGGATGGATTTAAGGCACTGACCTTGCTGGCCGTGACACTTAT
  TGTCCTCAACTCCACCCTGAAGAGCTTTGACCAGTTCACCTGCAACCTGCTGTACGTGAGCTGGAGGAAG
  GACCTCACGGAGCACCTGCACCACCTGTACTTCCGGGCCCGCGTGTACTACACCCTCAACGTGCTGCGGG
  ACGACATTGATAATCCGGACCAGCGAATCAGCCAGGATGTGGAGCGATTCTGCCGCCAGCTCAGCAGCGT
  GACCAGCAAGCTGATCATTTCCCCCTTCACTCTTACCTACTACACTTACCAGTGCTTCCAAAGCACAGGC
  TGGCTCGGGCCTGTGAGCATCTTTGGATATTTCACCGTGGGGACCATGGTGAACAAAACTTTGATGGGGC
  CCATCGTGACGAAGCTGGTGCAGCAGGAGAAGCTGGAGGGGGATTTTAGGTTCAAGCACATGCAGATTCG
  AGTGAATGCAGAGCCTGCGGCTTTCTATAGAGCTGGGCTCGTCGAGCACATGAGGACAGACCGCAGGCTG
  CAGAGACTCCTTCAGACCCAGAGAGAGCTGATGTCCAGGGAGCTCTGGCTGTACATTGGTATCAACACCT
  TTGACTATCTGGGCAGCATCCTGAGTTATGTCGTCATCGCTATCCCCATTTTCAGTGGGGTCTATGGAGA
  TTTGAGCCCCACAGAGCTTAGCACCCTGGTCAGCAAGAATGCCTTTGTGTGCATCTACCTCATCAGCTGC
  TTCACCCAGCTCATTGACCTGTCCACCACACTCTCGGATGTTGCCGGTTACACACACAGGATTGGAGAGC
  TTCAGGAGGCCCTGCTGGACATGTCCCGAAAATCACAAGACTGTGAAGCCCTAGGCGAGAGTGAGTGGGA
  CTTGGACAAAACCCCAGGGTGTCCAACAACAGAGCCATCAGACACAGCTTTTCTGCTCGATCGGGTTTCC
  ATATTGGCCCCCTCCTCTGACAAGCCCCTGATCAAGGACTTGAGCTTGAAGATCTGTGAGGGGCAGAGTC
  TGCTCATCACAGGCAACACGGGCACTGGCAAGACCTCCCTGCTGCGGGTGCTGGGAGGCCTGTGGGAGGG
  CATGAAAGGCTCAGTGCAGATGCTGGCTGATTTTGGGCCCCACGGGGTGCTGTTCCTGCCTCAGAAGCCA
  TTCTTCACTGATGGGACACTTCGGGAGCAGGTGATATATCCCCTGAAGGAGATCTACCCTGACTCAGGTT
  CTGCGGACGATGAGAGGATTGTGAGGTTCTTGGAATTGGCAGGCCTGTCCAGCTTGGTGGCAAGGACTGG
  AGGTCTGGACCAGCAGGTGGATTGGAACTGGTATGATGTCCTGTCCCCAGGGGAGATGCAAAGACTCTCC
  TTCGCCCGTCTCTTCTACCTGCAGCCCAAGTATGCAGTTTCACTCCTGGGTTCTGAGACTCCACGGAGGA
  GGAAGCTGGGAGCTGAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR208310 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MTLLCVTLLEQLVIYQVGLIPSQYYGVLGNKDLDGFKALTLLAVTLIVLNSTLKSFDQFTCNLLYVSWRK
  DLTEHLHHLYFRARVYYTLNVLRDDIDNPDQRISQDVERFCRQLSSVTSKLIISPFTLTYYTYQCFQSTG
  WLGPVSIFGYFTVGTMVNKTLMGPIVTKLVQQEKLEGDFRFKHMQIRVNAEPAAFYRAGLVEHMRTDRRL
  QRLLQTQRELMSRELWLYIGINTFDYLGSILSYVVIAIPIFSGVYGDLSPTELSTLVSKNAFVCIYLISC
  FTQLIDLSTTLSDVAGYTHRIGELQEALLDMSRKSQDCEALGESEWDLDKTPGCPTTEPSDTAFLLDRVS
  ILAPSSDKPLIKDLSLKICEGQSLLITGNTGTGKTSLLRVLGGLWEGMKGSVQMLADFGPHGVLFLPQKP
  FFTDGTLREQVIYPLKEIYPDSGSADDERIVRFLELAGLSSLVARTGGLDQQVDWNWYDVLSPGEMQRLS
  FARLFYLQPKYAVSLLGSETPRRRKLGAD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC050102
• ORF Size: 1557 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC050102, AAH50102
• RefSeq Size: 2410 bp
• RefSeq ORF: 1559 bp
• Locus ID: 19300
• MW: 58.6 kDa
• Gene Summary: The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the ALD subfamily, which is involved in peroxisomal import of fatty acids and/or fatty acyl-CoAs in the organelle. All known peroxisomal ABC transporters are half transporters which require a partner half transporter molecule to form a functional homodimeric or heterodimeric transporter. The function of this peroxisomal membrane protein is unknown. However, it is speculated that the human protein may function as a heterodimer for another peroxisomal ABC transporter and, therefore, may modify the adrenoleukodystrophy phenotype. It may also play a role in the process of peroxisome biogenesis. [provided by RefSeq, Jul 2008]