Pole2 (NM_011133) Mouse Tagged ORF Clone

CAT#: MR208447

Pole2 (Myc-DDK-tagged) - Mouse polymerase (DNA directed), epsilon 2 (p59 subunit) (Pole2)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Pole2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR208447 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGCCGGAGCGGCTGCGGAGCCGCACGGTCTCAGCTTTCAAGCTGCGGGGCTTGCTGCTCAGGAGTG
  AAGCCACCAAGTATCTGACTGAAGCTCTCCAGTCTGTCACTGAATTGGAGCTAGAGGAGAACCTAGGAAA
  GATCATCGATGCAGTTGAAAAGCAGCCCTTGTCCTCGAACATGATCGAACGATCTGTCGTGGAGGCAGCT
  GTGCAAGAGTGCAGTCAGTCTGTGGATGAGACTATAGAGCACGTTTTCAATATCATTGGAGCTTTTGATA
  TTCCACGTTTTATATATAATTCAGAAAGGAAAAAATTTCTTCCTCTATCCATGACTAATCATGCTGCACC
  AACCTTATTTGGAACAGCAAGAGACAAAGCCGAGCTGTATCTTGAACGCTATACCATTTTACACCAGAGG
  ACACACAGACATGAATTGTTCACTCCTCCGGTGATAGGCTCACACCTTGAAGAAAGTGGGAGCAAGTTCC
  AGCTTAAAACTATAGAAACCTTACTGGGTAGCACAAGCAAAGTTGGTGACGTGATCGTCCTGGGGATGAT
  AACCCAGCTGAAAGAGGGGAAGTTTTTCCTGGAAGATCCTACTGGAACAGTACAACTGGACCTTAGTAAA
  GCTCAGTTCCACAGTGGCTTATACACCGAGGCCTGCTTCGTCCTGGCAGAAGGTTGGTTTGAAGATCAAG
  TGTTTCACGCCAATGCCTTTGGATTTCCTCCCACTGAGCCCTCCAGCACCACAAGGGCGTACTATGGAAA
  TATTAATTTTTTTGGAGGGCCTTCTAATGCATCAGTGAAGACGTATACAAAGCTGAGACAGCTGGAAGAT
  GAGAACAAGGACGCCATGTTTGTGATTGTGTCTGATGTTTGGTTGGACCAAGTGCAAGTGTTGGAGAAGT
  TTCATGTAATGTTTTCTGGTTACTCGCCAGCACCTCCAACCTGCTTTATTCTGTGTGGTAATTTTTCATC
  TGCACCATATGGAAAAAATCAAGTCCAGGCTTTGAAAGATTCCCTAAAATCTTTGGCAGACATAATATGT
  GAATATCCAAGTATCCACCAAAGTAGCCGTTTTGTGTTTGTGCCTGGCCCAGAGGATCCTGGATTTGGTT
  CCATTTTACCAAGGCCGCCACTCGCTGAAAGCATCACACAGGAATTCAGACAAAGGGTTCCGTTTTCAGT
  TTTCACGACTAATCCTTGCAGAATTCAGTATTGTACACAAGAAATAATTATCTTCCGTGAAGACTTAGTG
  AATAAAATGTGTAGAAACTGTGTCCGTTTTCCTAGCAGCAGTTTGGATATTCCTACTCACTTCGTAAAGA
  CCATCTTATCTCAGGGACATCTGGCTCCTCTCCCTCTTTACGTATGCCCCGTCCACTGGGCATATGATTA
  CACGTTGAGAGTGTACCCGGTGCCCGACCTGCTGGTCATTGCAGACAAGTACGACCCGTTCACCGTGACC
  AACACCGATTGCCTCTGCATAAACCCTGGCTCCTTTCCAAGAAGTGGATTTGCATTCAAAGTTTTTTACC
  CTTCCAGTAAAACAGTAGAAGACAGCAAACTTCAGGGCTTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR208447 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAPERLRSRTVSAFKLRGLLLRSEATKYLTEALQSVTELELEENLGKIIDAVEKQPLSSNMIERSVVEAA
  VQECSQSVDETIEHVFNIIGAFDIPRFIYNSERKKFLPLSMTNHAAPTLFGTARDKAELYLERYTILHQR
  THRHELFTPPVIGSHLEESGSKFQLKTIETLLGSTSKVGDVIVLGMITQLKEGKFFLEDPTGTVQLDLSK
  AQFHSGLYTEACFVLAEGWFEDQVFHANAFGFPPTEPSSTTRAYYGNINFFGGPSNASVKTYTKLRQLED
  ENKDAMFVIVSDVWLDQVQVLEKFHVMFSGYSPAPPTCFILCGNFSSAPYGKNQVQALKDSLKSLADIIC
  EYPSIHQSSRFVFVPGPEDPGFGSILPRPPLAESITQEFRQRVPFSVFTTNPCRIQYCTQEIIIFREDLV
  NKMCRNCVRFPSSSLDIPTHFVKTILSQGHLAPLPLYVCPVHWAYDYTLRVYPVPDLLVIADKYDPFTVT
  NTDCLCINPGSFPRSGFAFKVFYPSSKTVEDSKLQGF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_011133
• ORF Size: 1584 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_011133.1, NM_011133.2, NP_035263.1
• RefSeq Size: 1695
• RefSeq ORF: 1584
• Locus ID: 18974
• MW: 59.4 kDa
• Gene Summary: Participates in DNA repair and in chromosomal DNA replication. [UniProtKB/Swiss-Prot Function]