Mtmr2 (BC063050) Mouse Tagged ORF Clone

CAT#: MR208990

Mtmr2 (Myc-DDK-tagged) - Mouse myotubularin related protein 2 (cDNA clone MGC:66658 IMAGE:5716297)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Mtmr2
• Synonyms: 6030445P13Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR208990 representing BC063050
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGAGCGAGTTTCCGGGTCCAGGCTCTCGGGTCCCCTGGCGGCCTAGAGACGAGGCGCTGCGCGTGA
  ACGTGGGCGGAGTGCGGCGGCTGCTGAGCGCGCGCGCCCTTGCGCGCTTCCCGGGCACGCGCCTGGGCCG
  CCTACAGGCGGCGGCGTCCGAGGAGCAGGCGCGGCGCCTGTGCGACGACTACGACGCAGCGGCGCACGAG
  TTCTACTTTGATCGGCATCCGGGCTTCTTTCTCGGCCTCCTACACTTCTACCGCACCGGGCACCTGCACG
  TCCTAGACGAGCTGTGCGTCTTCGCCTTCGGCCAGGAGGCTGACTACTGGGGCCTGGGCGAGAACGCGCT
  GGCCACGTGCTGCCGCGCGCGGTATCTGGAGCGGCGTGTGGCGCGGCCTCGCGCCTGGGACGAGGACAGC
  GACGCGCCGAGCAGCGTGGACCCGTGTCCCGACGAGATCTCGGACGTGCAGCGGGAGCTGGCGCGCTATG
  GTGCGGCTCGCTGTGGCCGCCTGCGCCGTCGTCTCTGGCTCACCATGGAGAATCCAGGCTACTCGCTGCC
  CAGCAAGCTCTTCAGCTGCGTATCCATCGGCGTGGTGCTCGCCTCCATCGCTGCCATGTGCATCCACAGC
  CTGCCGGAGTACCAAGCTCGGGAGGCGGCGGCGGCGGTGGCTGCAGTGGCCGCGGGTCGCAGCGCAGAGG
  AGGTGCGCGACGACCCGGTGCTGCGCCGCCTGGAGTACTTCTGCATCGCTTGGTTCAGCTTCGAGGTGTC
  GTCGCGCCTGCTGCTGGCTCCCAGCACGCGCAACTTCTTCTGCCACCCGCTCAACCTCATTGACATCGTG
  TCGGTGCTGCCCTTCTATCTCACACTGCTGGCTGGCGCAGCGCTTGGTGACCAGCGCGGAGCCAGCGGGG
  AGGAGCTCGGGGACCTGGGCAAGGTAGTGCAAGTGTTCCGCCTCATGCGCATCTTCCGCGTGCTCAAGCT
  GGCGCGCCACTCCACGGGGCTGCGTTCGCTGGGCGCCACGCTCAAGCACAGCTACCGTGAGGTGGGCATC
  TTACTGCTGTACCTGGCCGTGGGTGTGTCAGTGTTCTCCGGCGTGGCCTACACAGCCGAAGAAGAAAACG
  AGGGCTTTCACACAATCCCTGCCTGCTGGTGGTGGGGCACAGTGAGCATGACCACAGTGGGCTATGGGGA
  TGTGGTGCCAGAGACTGTGGGTGGCAAGCTGGCGGCCTCGGGCTGCATCCTCGGGGGCATCCTGGTGGTC
  GCCCTCCCCATCACCATCATCTTCAACAAGTTTTCCCACTTCTACCGGCGCCAGAAGGCACTGGAGGCGG
  CCGTGCGGAGCAGCGGTCAGCGCGAGTTTGAGGACTTGCTGAGTAGCGTTGACGGGGTATCGGATGTGTC
  TCTGGAAACATCCCGGGACACTTCTCAGGAGGGACGCTCTACAGACCTGGAGACCCAAGCTCCCCGGGAG
  CCTGCAAAATCTCACAGTTAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR208990 representing BC063050
  Red=Cloning site Green=Tags(s)
  
  MVSEFPGPGSRVPWRPRDEALRVNVGGVRRLLSARALARFPGTRLGRLQAAASEEQARRLCDDYDAAAHE
  FYFDRHPGFFLGLLHFYRTGHLHVLDELCVFAFGQEADYWGLGENALATCCRARYLERRVARPRAWDEDS
  DAPSSVDPCPDEISDVQRELARYGAARCGRLRRRLWLTMENPGYSLPSKLFSCVSIGVVLASIAAMCIHS
  LPEYQAREAAAAVAAVAAGRSAEEVRDDPVLRRLEYFCIAWFSFEVSSRLLLAPSTRNFFCHPLNLIDIV
  SVLPFYLTLLAGAALGDQRGASGEELGDLGKVVQVFRLMRIFRVLKLARHSTGLRSLGATLKHSYREVGI
  LLLYLAVGVSVFSGVAYTAEEENEGFHTIPACWWWGTVSMTTVGYGDVVPETVGGKLAASGCILGGILVV
  ALPITIIFNKFSHFYRRQKALEAAVRSSGQREFEDLLSSVDGVSDVSLETSRDTSQEGRSTDLETQAPRE
  PAKSHSY
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC063050
• ORF Size: 1491 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC063050, AAH63050
• RefSeq Size: 3125 bp
• RefSeq ORF: 1715 bp
• Locus ID: 77116
• MW: 114.5 kDa