Osbpl6 (BC022908) Mouse Tagged ORF Clone

CAT#: MR210095

Osbpl6 (Myc-DDK-tagged) - Mouse oxysterol binding protein-like 6 (cDNA clone MGC:30542 IMAGE:5043261)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Osbpl6
• Synonyms: MGC30542, ORP-6, mKIAA4128
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR210095 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAGCCCAACAGCTTTCCATGGCAGTCGCCGTTACCATGCAGTAACAGCCTCCCTGCGACCTGCACAA
  CTGGGCAGAGTAAAGTGGCAGCCTGGCTGCAGGACTCAGAAGAAATGGACAGGTGTGCAGAAGATCTTGC
  ACACTGCCAGTCAAACCTTGTGGAACTTAGCAAGCTCCTGCAAAACTTGGAGATCCTTCAGAGAACTCAG
  TCGGCGCCTAACTTCACTGACATGCAGGCTAACTGTGTAGATATTTCAAAGAAAGACAAGCGGGTCACAA
  GACGCTGGAGGACAAAAAGTGTCAGCAAAGATACAAAAATACAGCTCCAGGTTCCTTTCAGCGCCACCAT
  GTCACCCGTGCGTCTGCACTCCTCCAACCCCAACCTTTGTGCGGACATTGAGTTCCAGACCCCTCCTAGC
  CACCTCACCGACCCTCTGGAAAGTTCCACGGATTATACCAAGCTACAAGAAGAATTTTGTCTAATTGCAC
  AGAAAGTGCATTCTCTTCTGAAGTCTGCATTTAACAGCATAGCTATAGAGAAGGAGAAGCTGAAGCAGGT
  GGTCTCGGAGCAAGATCACAACAAAGGCCACAGCACACAGATGGCCAGACTCCGGCAGTCACTGTCACAG
  GCACTTAACCAGAACGCTGAGCTCCGGAGTCGGCTGAACAGAATACACTCAGAGTCTACCATTTGTGATC
  ACGTTGTCAGTGTAAATATTATCCCTAGCCCCGATGAGCCTGGTGAGCAAATCCATGTCAGCCTCCCTCT
  GTCACAGCAGGTGGCCAACGAGAGCCGCCTCTCCATGTCAGAATCCGTGTCAGAATTCTTCGATGCCCAA
  GAGGTGCTCCTGTCTGCAAGCTCATCAGAGAATGAGGCTTCCGATGATGAGTCCTACATCAGCGACGTGA
  GTGACAACATATCTGAGGACAACACCAGTGTCGCCGACAACATTTCTCGGCAAATCCTGAATGGGGAGCT
  CACAGGAGGCGCCTTCCGCAATGGCCGTCGCACCTGCCTGCCTGCTCCTTGCCCGGACACCAGTAACATT
  AACCTGTGGAATATCTTGAGGAACAACATTGGCAAAGACCTGTCGAAGGTCTCCATGCCCGTGGAACTCA
  ACGAGCCCCTCAACACCCTGCAGCACCTCTGTGAGGAAATGGAATACAGTGAGCTCCTGGACAAGGCCTC
  AGAGACCGACGACCCCTACGAGAGGATGGTTCTCGTCGCTGCCTTTGCAGTCTCAGGGTACTGTTCCACC
  TACTTCCGAGCAGGAAGCAAGCCATTCAATCCGGTCCTGGGGGAGACCTATGAATGCATAAGAGAAGATA
  AGGGGTTTCGGTTCTTCTCAGAACAAGTTAGCCATCATCCACCCATTTCTGCCTGTCACTGTGAATCCAA
  GAATTTTGTGTTTTGGCAAGATATCAGATGGAAAAATAAGTTCTGGGGAAAGTCCATGGAAATCCTGCCT
  GTCGGAACCCTGAACGTCACGCTTCCAAAATATGGGGATTACTATGTGTGGAATAAAGTCACCACGTGCA
  TACACAATATCCTGAGTGGGAGGAGATGGATCGAGCACTACGGTGAAGTGACCCTTAGAAATACCAAAAG
  CAGTGTTTGCATTTGCAAACTCACGTTTGTCAAGGTGAATTACTGGAACTCCAACGTGAATGAAGTCCAG
  GGGGTGGTGATAGACCAGGAGGGGAAGGTGGTGCACCGGCTGTTTGGGAAATGGCACGAAGGACTCTACT
  GCGGGGTGGCCCCCTCGGCAAAGTGCATCTGGAGACCAGGTTCTCTGCCAACCAACTATGAGCTCTACTA
  TGGCTTTACAAGATTTGCTGTGGAGCTCAATGAGTTGGACCCCGTCCTGAAGGACCTCCTTCCCCCCACA
  GATGCCCGATTCCGGCCAGATCAAAGGTTTTTGGAAGAAGGGAACCTAGAAGCCGCAGCCGCAGAGAAAC
  AAAGAGTTGAGGAACTCCAAAGATCGCGAAGACGCTACATGGAGGAAAACAACCTTGAACATATACCAAA
  ATTTTTTAAAGCTTGTGTCTGCTCAGATTTGGCCCCACTGTGGTTAGACCAAAGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR210095 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MQPNSFPWQSPLPCSNSLPATCTTGQSKVAAWLQDSEEMDRCAEDLAHCQSNLVELSKLLQNLEILQRTQ
  SAPNFTDMQANCVDISKKDKRVTRRWRTKSVSKDTKIQLQVPFSATMSPVRLHSSNPNLCADIEFQTPPS
  HLTDPLESSTDYTKLQEEFCLIAQKVHSLLKSAFNSIAIEKEKLKQVVSEQDHNKGHSTQMARLRQSLSQ
  ALNQNAELRSRLNRIHSESTICDHVVSVNIIPSPDEPGEQIHVSLPLSQQVANESRLSMSESVSEFFDAQ
  EVLLSASSSENEASDDESYISDVSDNISEDNTSVADNISRQILNGELTGGAFRNGRRTCLPAPCPDTSNI
  NLWNILRNNIGKDLSKVSMPVELNEPLNTLQHLCEEMEYSELLDKASETDDPYERMVLVAAFAVSGYCST
  YFRAGSKPFNPVLGETYECIREDKGFRFFSEQVSHHPPISACHCESKNFVFWQDIRWKNKFWGKSMEILP
  VGTLNVTLPKYGDYYVWNKVTTCIHNILSGRRWIEHYGEVTLRNTKSSVCICKLTFVKVNYWNSNVNEVQ
  GVVIDQEGKVVHRLFGKWHEGLYCGVAPSAKCIWRPGSLPTNYELYYGFTRFAVELNELDPVLKDLLPPT
  DARFRPDQRFLEEGNLEAAAAEKQRVEELQRSRRRYMEENNLEHIPKFFKACVCSDLAPLWLDQR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC022908
• ORF Size: 2085 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC022908, AAH22908
• RefSeq Size: 2688 bp
• RefSeq ORF: 2087 bp
• Locus ID: 99031
• MW: 79 kDa