Nsd3 (NM_001081269) Mouse Tagged ORF Clone

CAT#: MR216453

Whsc1l1 (Myc-DDK-tagged) - Mouse Wolf-Hirschhorn syndrome candidate 1-like 1 (human) (Whsc1l1), transcript variant 2

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Nsd3
• Synonyms: 6720429E03; A530023P05; AI528490; WHISTLE; Whsc1l1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR216453 representing NM_001081269
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATTTCTCTTTCTCTTTCATGCAAGGGATCATGGGAAACACAATTCAGCAACCACCTCAACTCATTG
  ACTCGGCCAACATCCGCCAGGAGGATGCCTTTGATAACCACAGTGACATTGTTGAAGATGGTGGCCCGAC
  ACCCTTTGAAGCTACTTTGCAACAAGGTTTTCAATATCCACCTACAACAGAAGACCTTCCTCCGCTCACA
  AATGGCTACCCACCATCAATCAGCTTGTATGAAACTCAAACCAAATACCCGCCATATAATCAGTATCCCA
  ATGGGTCAGCCAACGGTTTTGGTGCAGTTAGAAACTTTAGCCCTACTGACTATTACCATTCAGAAATTCC
  AAACACAAGACCACATGAAATTCTGGAAAAACCTTCTCCTCCTCAGCCACCTCCTCTTCCTCCTCCTCCT
  CCTCCTCCTCCTCCTCCTCCTTCGGTACCACAAACTGTGATTCCAAAGAAGACAGGCTCACCCGAGATTA
  AACTAAAAATAACCAAAACTATCCAGAATGGCAGGGAATTGTTTGAGTCTTCCCTTTGTGGAGACCTCTT
  AAATGAAGTACAGGCAAGCGAGCACACAAAGTCTAAGCATGAAAGCAGAAAGGAAAAGAGGAAAAAGAGC
  AACAGACACGAGTCATCTCGATCCGAAGAGCGGAGGTCACACAAGATTCCCAAGCTAGAGCCGGAGGGAC
  AGAATAGACCAAATGAGAGGGTGGACACTGCACCAGAGAAGCCAAGAGAAGAGCCAGTGCTCAAAGAGGC
  CATCCCGGTTCAGCCAATACTGTCTTCTGTTCCAACAACAGAAACATCCACTGGTGTTAAGTTCCAGGTT
  GGTGATCTTGTTTGGTCCAAGGTGGGAACCTACCCTTGGTGGCCTTGTATGGTTTCAAGTGATCCCCAGC
  TTGAGGTCCATTCCAAAATTAACACAAGAGGTGCCCGGGAATATCATGTCCAATTTTTTAGCAACCAGCC
  AGAGAGGGCATGGGTTCATGAGAAACGGGTACGGGAATACAAAGGTCATGAACAGTATGAAGAGTTACTA
  GCCGAGGCAGCCAAGCAAGCCAGCAATCATTCTGAAAAGCAAAAGATTCGGAAACCCCGACCTCAGAGAG
  AACGTGCCCAATGGGACATTGGCATTGCTCATGCAGAGAAAGCATTGAAAATGACTCGGGAGGAAAGAGT
  AGAGCAGTACACTTTCATCTACATTGATAAGCAGCCAGAAGAGGCTTCGTCCCAAGCAAAGAAGAATGTT
  ACCTCTAAGACGGAAGTCAAGAAACCCCGAAGACCAAGATCTGTGCTGAACAGTCAGCCAGAACAGACCA
  ATGCTGGGGAGGTGGCCTCCTCACAATCAAGTACTGACCTTCGAAGGCAGAGCCAGAGGCGGCATACTAG
  CTTGGAAGAGGAAGAGCCACCTCCTGTTAAAATCGCCTGGAAAACAGCAGCCGCAAGGAAGTCCTTACCA
  GCCTCCATCACAATGCACAAAGGGAGCCTAGATTTGCAGAAGTGTAATATGTCTCCAGTTGTGAAAATTG
  AACAAGTGTTTGCTCTCCAGAATGCAACAGGAGATGGGAAGTTTATTGATCAGTTTGTTTATTCAACGAA
  GGGTATTGGTAACAAAACAGAAATAAGTGTCAGGGGGCAAGACAGGCTTATAATCTCTTCACCAAGTCAG
  AGAAGTGAAAAGCCGGCTCAGAGCGCGTCATCTCCGGAGGCAACATCTGGTTCTGCAGGCCCAGTAGAGA
  AGAAGCAGCAGAGAAGATCCATCAGGACTCGATCTGAGTCAGAGAAGTCCGCCGAGGTTGTGCCAAAGAA
  GAAGATCAAAAAGGAGCAGGTTGAGACAGCTCCCCAGGCCTCCCTGAAGACCGGGTTACAGAAAGGTGCC
  AGCGAGATTTCAGATTCCTGTAAGCCCCTAAAGAAAAGGAGTCGCGCCTCAACGGATGTAGAGACGGCTA
  GTTGCACGTACAGAGACACATCTGACTCCGATTCTAGAGGACTGAGTGACGGGCAGGTAGGCTTTGGCAA
  ACAAGTAGATAGCCCGTCAGCTACTGCTGACGCAGATGCTTCTGACGCGCAGTCGGTGGACTCGAGTCTG
  TCACGGAGAGGTGTCGGCACGAGTAAGAAGGACACTGTGTGTCAGGTCTGTGAGAAGGCTGGCGACTGCC
  TTGTTGCTTGTGAGGGGGAGTGCTGCCGACACTTTCACGTGGAGTGCTTGGGACTGACTGCAGTCCCTGA
  GGGCCACTTCACCTGTGAGGAGTGTGAGACTGGACAGCACCCATGTTTTTCATGTAAAGTGTCTGGTAAG
  GATGTGAAACGTTGTTCTGTTAGTGTCTGTGGAAAGTTTTATCATGAAGCCTGTGTCCGCAAATTCCCCA
  CTGCCATCTTTGAGTCAAAAGGATTCCGCTGTCCCCAGCACTGTTGCTCTTCCTGCTCTATGGAGAAAGA
  CATCCACAAAGCAAGTAAAGGCCGCATGATGAGGTGTCTGAGATGCCCGGTCGCCTATCACGTTGGAGAT
  GCGTGTGTCGCTGCTGGAAGTGTATCTGTGTCCTCGCACATTCTCATCTGTAGTAACCATTCCAAGCGGA
  GTAGTCAGTCTGCTGCTATAAACGTAGGCTTTTGTTTCGTTTGTGCCAGAGGGCTGATAGTTCAGGACCA
  TTCAGACCCCATGTTCAGTTCCTATGCCTATAAATCCCACTACCTACTGAGTGAATCAAACCGTGCTGAG
  TTGATGAAATTACCTATGATTCCTTCTTCGTCAGCTTCCAAAAAGAGATGTGAGAAAGGTGGAAGATTGC
  TCTGCTGTGAATCGTGCCCTGCTTCCTTCCACCCAGAATGCCTGAGCATAGACATGCCAGAAGGCTGCTG
  GAATTGCAATGACTGTAAAGCTGGCAAGAAACTGCATTACAAACAGATTGTTTGGGTCAAATTGGGAAAT
  TACAGATGGTGGCCTGCAGAGATTTGCAGTCCCAGGTCTGTGCCGCTGAACATCCAGGGCCTTAAGCATG
  ACTTGGGGGACTTCCCCGTGTTCTTCTTTGGTTCTCACGACTACTACTGGGTTCACCAAGGCAGAGTGTT
  CCCTTACGTTGAAGGAGACAAACACTTTGCAGAGGGACAGACTAGCATTAACAAGACCTTCAAAAAAGCA
  CTGGAAGAAGCTGCAAAACGTTTCCAGGAGCTGAAAGCCCAGCGTGAAAGCAAGGAGGCTTTGGAGATGG
  AGAGGACCTCCAGGAAACCCCCTCCCTACAAGCACATCAAGGCCAACAAAGTCATAGGGAAGGTGCAGGT
  CCAGGTGGCTGACCTGTCGGAGATCCCCCGCTGTAACTGCAAGCCTGGTGATGAGAACCCGTGTGGCCTG
  GAGTCGCAGTGTCTGAACAGGATGTCACAGTATGAGTGTCACCCGCAGGTGTGTCCTGCTGGGGACCGCT
  GTCAGAACCAGTGCTTTACAAAGAGGCTGTACCCCGATGCAGAGGTCATCAAGACCGAGCGGCGAGGATG
  GGGCCTCAGAACCAAAAGAAGCATCAAAAAGGGGGAATTTGTAAACGAGTATGTCGGTGAATTAATTGAT
  GAAGAAGAGTGCAGACTCCGAATCAAGCGAGCCCACGAGAACAGTGTAACTAATTTTTATATGTTAACTG
  TTACCAAGGACCGTATAATTGATGCTGGACCAAAGGGAAATTATTCTCGTTTCATGAACCACAGTTGTAA
  TCCAAACTGTGAGACACAGAAGTGGACTGTAAATGGAGACGTTCGAGTTGGACTATTTGCTCTTTGTGAC
  ATTCCTGCAGGGATGGAGTTAACGTTTAACTATAACTTGGACTGTCTGGGCAACGGCAGGACAGTGTGCC
  ACTGCGGAGCAGACAACTGTAGTGGATTTCTAGGAGTGCGGCCAAAGTCTGCATGCACATCAGCAGTTGA
  CGAGAAGACAAAAAATGCCAAGTTGAAGAAGAGGCGGAAAGTCAAAGCGGAGGCAAAGCCAATCCATGAG
  GATTATTGTTTTCAGTGTGGGGATGGTGGAGAACTGGTCATGTGTGACAAGAAAGACTGTCCCAAAGCTT
  ACCACCTCCTATGCCTTAACCTGACTCAGCCACCCCACGGAAAGTGGGAGTGCCCGTGGCATCGGTGTGA
  CGAGTGTGGCAGTGTAGCCGTTTCCTTCTGTGAATTCTGTCCACACTCATTCTGTAAGGCTCATGGAAAA
  GGGGCTCTGGTGCCCTCTGCACTAGAAGGTCGTCTCTGCTGCTCCAGCCACGACCCTGCATCTCCTGTGT
  CACCAGAATACTGGAGCAAGATCAGATGTAAATGGGAATCCCAAGATAGTGGAGAAGAAGTAAAGGAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR216453 representing NM_001081269
  Red=Cloning site Green=Tags(s)
  
  MDFSFSFMQGIMGNTIQQPPQLIDSANIRQEDAFDNHSDIVEDGGPTPFEATLQQGFQYPPTTEDLPPLT
  NGYPPSISLYETQTKYPPYNQYPNGSANGFGAVRNFSPTDYYHSEIPNTRPHEILEKPSPPQPPPLPPPP
  PPPPPPPSVPQTVIPKKTGSPEIKLKITKTIQNGRELFESSLCGDLLNEVQASEHTKSKHESRKEKRKKS
  NRHESSRSEERRSHKIPKLEPEGQNRPNERVDTAPEKPREEPVLKEAIPVQPILSSVPTTETSTGVKFQV
  GDLVWSKVGTYPWWPCMVSSDPQLEVHSKINTRGAREYHVQFFSNQPERAWVHEKRVREYKGHEQYEELL
  AEAAKQASNHSEKQKIRKPRPQRERAQWDIGIAHAEKALKMTREERVEQYTFIYIDKQPEEASSQAKKNV
  TSKTEVKKPRRPRSVLNSQPEQTNAGEVASSQSSTDLRRQSQRRHTSLEEEEPPPVKIAWKTAAARKSLP
  ASITMHKGSLDLQKCNMSPVVKIEQVFALQNATGDGKFIDQFVYSTKGIGNKTEISVRGQDRLIISSPSQ
  RSEKPAQSASSPEATSGSAGPVEKKQQRRSIRTRSESEKSAEVVPKKKIKKEQVETAPQASLKTGLQKGA
  SEISDSCKPLKKRSRASTDVETASCTYRDTSDSDSRGLSDGQVGFGKQVDSPSATADADASDAQSVDSSL
  SRRGVGTSKKDTVCQVCEKAGDCLVACEGECCRHFHVECLGLTAVPEGHFTCEECETGQHPCFSCKVSGK
  DVKRCSVSVCGKFYHEACVRKFPTAIFESKGFRCPQHCCSSCSMEKDIHKASKGRMMRCLRCPVAYHVGD
  ACVAAGSVSVSSHILICSNHSKRSSQSAAINVGFCFVCARGLIVQDHSDPMFSSYAYKSHYLLSESNRAE
  LMKLPMIPSSSASKKRCEKGGRLLCCESCPASFHPECLSIDMPEGCWNCNDCKAGKKLHYKQIVWVKLGN
  YRWWPAEICSPRSVPLNIQGLKHDLGDFPVFFFGSHDYYWVHQGRVFPYVEGDKHFAEGQTSINKTFKKA
  LEEAAKRFQELKAQRESKEALEMERTSRKPPPYKHIKANKVIGKVQVQVADLSEIPRCNCKPGDENPCGL
  ESQCLNRMSQYECHPQVCPAGDRCQNQCFTKRLYPDAEVIKTERRGWGLRTKRSIKKGEFVNEYVGELID
  EEECRLRIKRAHENSVTNFYMLTVTKDRIIDAGPKGNYSRFMNHSCNPNCETQKWTVNGDVRVGLFALCD
  IPAGMELTFNYNLDCLGNGRTVCHCGADNCSGFLGVRPKSACTSAVDEKTKNAKLKKRRKVKAEAKPIHE
  DYCFQCGDGGELVMCDKKDCPKAYHLLCLNLTQPPHGKWECPWHRCDECGSVAVSFCEFCPHSFCKAHGK
  GALVPSALEGRLCCSSHDPASPVSPEYWSKIRCKWESQDSGEEVKE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001081269
• ORF Size: 4338 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001081269.1, NM_001081269.2, NP_001074738.1
• RefSeq Size: 9945
• RefSeq ORF: 4341
• Locus ID: 234135
• MW: 162.1 kDa
• Gene Summary: This gene encodes a member of the SET domain family of histone lysine N-methyltransferase proteins. This protein methylates histone H3 at lysine residues 4 and 27, which represses gene transcription. It acts in opposition to the histone demethylase Jmjd1c. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, May 2015]