Spam1 (NM_009241) Mouse Tagged ORF Clone

CAT#: MR219759L4

Lenti ORF clone of Spam1 (mGFP-tagged) - Mouse sperm adhesion molecule 1 (Spam1), transcript variant 2

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: mGFP
• Symbol: Spam1
• Synonyms: AV039194; Ph-20
• Vector: pLenti-C-mGFP-P2A-Puro
• E. coli Selection: Chloramphenicol (34 ug/mL)
• Mammalian Cell Selection: Puromycin
• Sequence Data:
  ORF Nucleotide Sequence

  The ORF insert of this clone is exactly the same as(MR219759).
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NM_009241
• ORF Size: 1536 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_009241.3, NP_033267.2
• RefSeq Size: 2107
• RefSeq ORF: 1539
• Locus ID: 20690
• Gene Summary: Involved in sperm-egg adhesion. Upon fertilization sperm must first penetrate a layer of cumulus cells that surrounds the egg before reaching the zona pellucida. The cumulus cells are embedded in a matrix containing hyaluronic acid which is formed prior to ovulation. This protein aids in penetrating the layer of cumulus cells by digesting hyaluronic acid. [UniProtKB/Swiss-Prot Function]