Adam22 (NM_001007220) Mouse Tagged ORF Clone

CAT#: MR222778

Adam22 (Myc-DDK-tagged) - Mouse a disintegrin and metallopeptidase domain 22 (Adam22), transcript variant 1

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Adam22
• Synonyms: 2900022I03Rik; AI854032; MDC2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR222778 representing NM_001007220
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAGGCAGCGGCGGCCGCGTCCTTCTGGCTGCTCTGCGTCTTGGGGACCTGCCCCCTGGCGCGCTGCG
  GTCGGGCAGGAGTCGCCTCACTGAAAGGACTGGAGAGGGGGAAAGAAAACCGCTTCCTGGAGCGCCAGAG
  CATCATACCACTGCGCCTCATCTATCGCTTGGGCGGCGAGGATGAAACTCAGCACAACCAGCTCGACACG
  CGGGTGCGGGGCGACCCTGGCGGACCGCAGCTGACGCATGTGGACAAGGCAAGCTTCCGGGTGGACGCCT
  TTGGAACCTCTTTCGTTCTCGACGTCCTGCTGAACCACGAGTTGCTGTCGTCAGGTTATGTAGAGAGACA
  GATTGAACATGGAGGCAAGGTCGTGGAGAACAAAGGAGGAGAACACTGTTATTACCAGGGCCAGATTCGA
  GGAAACCCTGTCTCATTTGTTGCCCTATCAACATGCCATGGACTCCATGGGATGTTCTATGATGGAAACC
  ACACATATCTCATTGAGCCAGAAGAAAATGAGAAGTCTCAAGAGTCGTCTCATTGTCACTCCGTGTACAA
  ATCCAGACAGTTTGAATTTCCCTTGGATGACCTTCCGTCTGAATTTCAACGCGTAAACATTACTCCCCCT
  CAGTTTATTTTGAAGCCAAGACTAAAGAGGAGAAAAAGACAGCTTCTTCGATTTCCTCGGAATGTAGAGG
  AGGAAACCAAATACATCGAACTGATGATTGTGAATGATCATCTCATGTTTAAAAAGCACCGGCTTTCTGT
  TGTATATACTAATACCTATGCGAAGTCTGTGGTGAACATGGCAGATGTGATCTATAAAGACCAACTGAAA
  ACTAGGATTGTATTGGTTGCCATGGAAACCTGGGCTGCTGACAACAAGTTTGCCATATCTGAAAACCCAC
  TGATTACCCTTCGTGAGTTCATGAAATACAGGAGGGATTTTATCAAGGAGAAGGCTGATGCAGTTCATCT
  TTTCTCGGGCAGTCAATTTGAGAGTAGCCGGAGCGGGGCAGCTTATATTGGTGGGATTTGCTCGCTGCTG
  AGAGGAGGAGGCGTGAATGAATTTGGAAAAACGGATTTAATGGCAGTTACCCTCGCTCAGTCACTAGCCC
  ACAATGTCGGTATAATCTCTGACAAAAGAAAACTAGCAAGTGGTGAGTGTAAATGTGAGGACACCTGGTC
  CGGGTGCATAATGGGAGACACCGGCTACTATCTCCCGAAAAAGTTCACTCAGTGCAATGTCGAGGAATAT
  CATGACTTCCTGAATAGTGGTGGAGGTGCCTGCCTGTTCAACAAGCCTTCCAAGCTTCTTGATCCTCCTG
  AGTGTGGAAACGGCTTCATTGAAACCGGAGAGGAGTGTGATTGTGGCACCCCTGCAGAATGTGCCCTTGA
  AGGGGCAGAGTGTTGTAAGAAATGCACCTTGACCCAGGATTCTCAATGCAGTGATGGCCTTTGTTGTAAA
  AAGTGCAAGTTTCAGCCTCTGGGGACCGTGTGCCGAGAGGCAGTGAACGACTGTGACATTCGTGAAATAT
  GTTCAGGAAATTCGAGCCAGTGTGCCCCCAATGTGCATAAAATGGATGGATATTCGTGTGATGGTACTCA
  GGGAATTTGCTTTGGAGGAAGATGTAAAACCAGAGATAGACAATGCAAATACATCTGGGGGCAAAAGGTA
  ACAGCATCTGACAGGTACTGCTATGAGAAACTGAACATTGAGGGGACGGAGAAGGGTAACTGTGGGAAAG
  ACAAGGACACGTGGACACAGTGCAACAAACGGGATGTACTTTGTGGTTACCTCCTGTGCACAAACATTGG
  CAACATCCCAAGGCTCGGGGAGCTTGATGGTGAGATCACATCCACATTAGTTGTGCAGCAAGGAAGAACA
  TTAAACTGCAGTGGCGCGCATGTTAAACTTGAAGAAGATGTGGATCTTGGCTATGTGGAAGATGGGACGC
  CCTGTGGCCCTCAGATGATGTGCTTGGAACACAGGTGTCTTCCTGTGGCTTCCTTCAACTTCAGTACTTG
  CTCCAGCAGTAAAGCAGGCACCGTGTGCTCAGGAAATGGAGTTTGCAGCAATGAGCTCAAGTGTGTGTGC
  AACAGGCACTGGACAGGGGCTGACTGCGGCACCCACTTTCCTCACAATGATGATGCAAAGACTGGCATCA
  CCCTGTCTGGCAATGGTGTTGCTGGCACTAATATCATAATAGGAATAATTGCCGGCACCATTTTAGTGCT
  GGCCCTCATCTTAGGAATAACTGCCTGGGGTTATAAAAACTACCGAGAACAGAGACAGTTACCCCAGGGA
  GATTATGTAAAAAAGCCTGGCGATGGCGACTCTTTTTATAGCGACTTTCCTCCCGGAGGCAGCACAAACT
  CAGCATCTAGTTCTAAGAAGAGGTCAAATGGACTCTCTCATTCTTGGAGTGAAAGGATTCCAGACACAAA
  ACATATTTCAGACATCTGTGAAAATGGGAGACCTCGCAGTAACTCCTGGCAAGGTAACATGGGAGGCAAC
  AAAAAGAAAATCAGAGGGAAAAGATTTAGACCTCGATCTAACTCAACTGAG
  
  CTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATTACAAGGATGACGACG
  ATAAGGTTTAA
  Protein Sequence

  >MR222778 representing NM_001007220
  Red=Cloning site Green=Tags(s)
  
  MQAAAAASFWLLCVLGTCPLARCGRAGVASLKGLERGKENRFLERQSIIPLRLIYRLGGEDETQHNQLDT
  RVRGDPGGPQLTHVDKASFRVDAFGTSFVLDVLLNHELLSSGYVERQIEHGGKVVENKGGEHCYYQGQIR
  GNPVSFVALSTCHGLHGMFYDGNHTYLIEPEENEKSQESSHCHSVYKSRQFEFPLDDLPSEFQRVNITPP
  QFILKPRLKRRKRQLLRFPRNVEEETKYIELMIVNDHLMFKKHRLSVVYTNTYAKSVVNMADVIYKDQLK
  TRIVLVAMETWAADNKFAISENPLITLREFMKYRRDFIKEKADAVHLFSGSQFESSRSGAAYIGGICSLL
  RGGGVNEFGKTDLMAVTLAQSLAHNVGIISDKRKLASGECKCEDTWSGCIMGDTGYYLPKKFTQCNVEEY
  HDFLNSGGGACLFNKPSKLLDPPECGNGFIETGEECDCGTPAECALEGAECCKKCTLTQDSQCSDGLCCK
  KCKFQPLGTVCREAVNDCDIREICSGNSSQCAPNVHKMDGYSCDGTQGICFGGRCKTRDRQCKYIWGQKV
  TASDRYCYEKLNIEGTEKGNCGKDKDTWTQCNKRDVLCGYLLCTNIGNIPRLGELDGEITSTLVVQQGRT
  LNCSGAHVKLEEDVDLGYVEDGTPCGPQMMCLEHRCLPVASFNFSTCSSSKAGTVCSGNGVCSNELKCVC
  NRHWTGADCGTHFPHNDDAKTGITLSGNGVAGTNIIIGIIAGTILVLALILGITAWGYKNYREQRQLPQG
  DYVKKPGDGDSFYSDFPPGGSTNSASSSKKRSNGLSHSWSERIPDTKHISDICENGRPRSNSWQGNMGGN
  KKKIRGKRFRPRSNSTE
  
  LEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-XhoI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001007220
• ORF Size: 2571 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001007220.1, NM_001007220.2, NM_001007220.3, NP_001007221.1
• RefSeq Size: 2777 bp
• RefSeq ORF: 2574 bp
• Locus ID: 11496
• Cytogenetics: 5 3.39 cM
• MW: 95.2 kDa
• Gene Summary: This gene encodes a member of a disintegrin and metalloprotease (ADAM) family of endoproteases that play important roles in various biological processes including cell signaling, adhesion and migration. The encoded preproprotein undergoes proteolytic processing to generate a mature, functional protein. The protein encoded by this gene is believed to lack metalloproteinase activity due to the lack of a critical catalytic motif. Mice lacking the encoded protein exhibit severe ataxia, hypomyelination and premature death. Alternative splicing results in multiple transcript variants encoding different isoforms, some of which may undergo similar processing. [provided by RefSeq, May 2016]