Chrna7 (NM_007390) Mouse Tagged ORF Clone

CAT#: MR224522

Chrna7 (Myc-DDK-tagged) - Mouse cholinergic receptor, nicotinic, alpha polypeptide 7 (Chrna7)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Chrna7
• Synonyms: Acra7; alpha7; nAchR
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR224522 representing NM_007390
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTGCGGCCGGCGGGGAGGCATCTGGCTGGCTCTGGCCGCGGCGCTGCTGCACGTGTCCCTGCAAGGCG
  AGTTCCAGAGGAGGCTGTACAAGGAGCTGGTCAAGAACTACAACCCGCTGGAGAGGCCGGTGGCCAACGA
  CTCGCAGCCGCTCACCGTGTACTTCTCCCTGAGCCTCCTGCAGATCATGGATGTGGATGAGAAGAACCAA
  GTTTTAACCACCAACATTTGGCTACAAATGTCTTGGACAGATCACTATTTGCAGTGGAACATGTCTGAGT
  ACCCCGGAGTGAAAAATGTTCGTTTTCCAGATGGCCAGATTTGGAAACCAGACATTCTCCTCTATAACAG
  TGCAGATGAACGCTTTGATGCCACATTCCACACCAACGTCTTGGTGAATGCATCTGGGCATTGCCAGTAT
  CTCCCTCCAGGCATATTCAAGAGCTCCTGCTACATCGATGTACGCTGGTTCCCTTTTGATGTGCAGCAGT
  GCAAACTGAAGTTTGGGTCCTGGTCCTATGGAGGGTGGTCCTTGGACCTGCAGATGCAAGAGGCAGATAT
  CAGCAGCTATATCCCCAATGGAGAATGGGATCTCATGGGAATCCCTGGCAAAAGGAATGAGAAGTTCTAT
  GAATGCTGCAAAGAGCCATACCCAGATGTCACCTACACAGTAACCATGCGCCGTAGGACACTCTACTATG
  GCCTCAACCTGCTCATTCCTTGTGTGCTCATTTCAGCCCTGGCTTTGCTGGTATTCTTGCTGCCTGCAGA
  CTCTGGAGAGAAAATCTCTCTTGGAATAACTGTCTTACTTTCTCTGACTGTCTTCATGCTGCTTGTGGCT
  GAGATCATGCCAGCAACATCTGATTCCGTGCCCTTGATAGCACAGTACTTCGCCAGCACCATGATCATCG
  TGGGCCTCTCAGTGGTCGTGACAGTGATTGTGCTGCGATATCACCACCATGACCCTGATGGTGGCAAAAT
  GCCTAAGTGGACCAGGATCATTCTTCTGAATTGGTGTGCATGGTTTCTGCGCATGAAGAGGCCGGGAGAG
  GACAAGGTGCGGCCGGCTTGTCAGCACAAGCCTCGGCGCTGCAGCCTGGCCAGTGTGGAGCTGAGCGCAG
  GTGCGGGGCCACCCACCAGCAATGGCAACCTGCTCTACATTGGCTTCCGAGGCCTGGAGGGCATGCACTG
  TGCCCCAACTCCAGACTCTGGGGTCGTGTGTGGTCGTTTGGCCTGCTCCCCAACACATGATGAGCACCTC
  ATGCATGGTACACACCCCTCTGATGGGGACCCCGACCTGGCCAAGATCCTGGAGGAGGTCCGCTACATCG
  CCAACCGCTTCCGCTGCCAGGATGAGAGTGAGGTGATCTGCAGTGAGTGGAAGTTTGCGGCCTGTGTGGT
  GGACCGCTTGTGCCTCATGGCCTTTTCGGTTTTTACCATCATCTGCACCATCGGCATCCTCATGTCAGCC
  CCAAACTTTGTGGAGGCTGTGTCCAAAGACTTTGCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR224522 representing NM_007390
  Red=Cloning site Green=Tags(s)
  
  MCGRRGGIWLALAAALLHVSLQGEFQRRLYKELVKNYNPLERPVANDSQPLTVYFSLSLLQIMDVDEKNQ
  VLTTNIWLQMSWTDHYLQWNMSEYPGVKNVRFPDGQIWKPDILLYNSADERFDATFHTNVLVNASGHCQY
  LPPGIFKSSCYIDVRWFPFDVQQCKLKFGSWSYGGWSLDLQMQEADISSYIPNGEWDLMGIPGKRNEKFY
  ECCKEPYPDVTYTVTMRRRTLYYGLNLLIPCVLISALALLVFLLPADSGEKISLGITVLLSLTVFMLLVA
  EIMPATSDSVPLIAQYFASTMIIVGLSVVVTVIVLRYHHHDPDGGKMPKWTRIILLNWCAWFLRMKRPGE
  DKVRPACQHKPRRCSLASVELSAGAGPPTSNGNLLYIGFRGLEGMHCAPTPDSGVVCGRLACSPTHDEHL
  MHGTHPSDGDPDLAKILEEVRYIANRFRCQDESEVICSEWKFAACVVDRLCLMAFSVFTIICTIGILMSA
  PNFVEAVSKDFA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_007390
• ORF Size: 1506 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_007390.1, NM_007390.2, NM_007390.3, NP_031416.3
• RefSeq Size: 2091
• RefSeq ORF: 1509
• Locus ID: 11441
• MW: 57.1 kDa
• Gene Summary: After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. The channel is blocked by alpha-bungarotoxin. [UniProtKB/Swiss-Prot Function]