Chat (NM_009891) Mouse Tagged ORF Clone

CAT#: MR225685

Chat (Myc-DDK-tagged) - Mouse choline acetyltransferase (Chat)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Chat
• Synonyms: B230380D24Rik; CHOACTase
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR225685 representing NM_009891
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCTATCCTGGAAAAGGTCCCCCCAAAGATGCCTGTACAAGCTTCTAGCTGTGAGGAGGTGCTGGACT
  TACCTAAGTTGCCAGTGCCCCCACTGCAGCAAACCCTGGCCACCTACCTTCAGTGCATGCAACACCTGGT
  ACCTGAAGAGCAGTTCAGGAAGAGCCAGGCCATTGTGAAGCGGTTTGGGGCCCCTGGTGGCCTGGGTGAG
  ACCCTGCAGGAAAAGCTCTTGGAGAGACAGGAGAAGACAGCCAATTGGGTCTCTGAATACTGGCTGAATG
  ACATGTATCTAAACAACCGCCTGGCCCTGCCAGTCAACTCTAGCCCTGCTGTGATCTTTGCTCGGCAGCA
  CTTCCAAGACACCAATGACCAGCTAAGGTTTGCAGCCAGCCTCATCTCTGGTGTGCTTAGCTACAAGGCT
  CTGCTGGACAGCCAATCCATTCCCACTGACTGGGCCAAGGGGCAGCTCTCAGGGCAGCCTCTCTGTATGA
  AGCAGTACTACAGACTCTTCTCATCATACCGGCTTCCTGGCCATACCCAGGACACACTGGTGGCCCAGAA
  GAGCAGTATCATGCCTGAGCCCGAGCATGTCATCGTGGCCTGCTGCAACCAGTTCTTTGTCTTGGATGTT
  GTCATTAATTTCCGCCGTCTCAGTGAGGGTGATCTGTTCACTCAGTTGAGAAAGATAGTCAAAATGGCGT
  CCAACGAGGATGAACGCCTGCCTCCAATCGGCCTGCTGACGTCAGACGGGAGGAGCGAGTGGGCCAAGGC
  CAGGACGGTCCTCTTAAAAGACTCCACCAACCGGGACTCCCTGGACATGATCGAGCGCTGCATCTGCCTG
  GTATGCCTGGATGGTCCAGGCACTGGAGACCTCAGTGACACACACAGGGCCCTCCAGCTCCTTCATGGTG
  GAGGCTGCAGCTTGAATGGAGCGAATCGTTGGTATGACAAGTCCCTGCAGTTTGTGGTGGGCCGAGATGG
  CACCTGCGGTGTGGTGTGTGAGCACTCCCCTTTTGATGGCATCGTCCTGGTGCAGTGCACGGAGCACCTG
  CTGAAACATATGATGACAGGCAACAAGAAGCTCGTCCGAGCTGACTCAGTGAGTGAACTCCCTGCTCCCA
  GAAGGCTGAGGTGGAAATGTTCCCCAGAAACTCAAGGCCATCTCGCCTCCTCGGCAGAGAAACTTCAAAG
  AATCGTAAAGAATCTGGATTTCATTGTTTATAAGTTTGACAACTATGGGAAAACATTTATTAAGAAGCAG
  AAATGCAGTCCTGATGGCTTCATCCAGGTGGCCCTCCAGCTGGCTTACTACAGGCTTTACCAGAGGCTGG
  TGCCCACCTATGAGAGTGCATCCATCCGCCGCTTCCAGGAAGGTCGGGTGGACAACATCAGATCGGCCAC
  TCCTGAGGCTCTGGCTTTTGTGCAAGCCATGACTGACCACAAGGCTGCCGTGCTGGCTTCTGAGAAACTG
  CAGCTGCTGCAGAGGGCCATCCAGGCCCAAACTGAGTACACAGTCATGGCCATAACCGGCATGGCCATTG
  ACAACCATCTTCTGGCACTGAGGGAGCTGGCCCGAGACCTGTGCAAAGAGCCACCTGAGATGTTCATGGA
  TGAAACATACCTGATGAGCAACCGGTTTATTCTCTCCACCAGCCAGGTGCCCACGACCATGGAGATGTTC
  TGCTGTTATGGCCCTGTGGTACCCAATGGGTATGGAGCCTGTTATAACCCCCAGCCTGAGGCCATCACCT
  TCTGCATCTCCAGCTTTCACGGCTGCAAAGAGACCTCATCTGTGGAGTTTGCAGAAGCGGTGGGAGCGAG
  CCTTGTTGACATGAGAGACCTCTGTAGTTCGAGGCAGCCTGCTGAAGGCAAGCCACCAACAGCAAAGGAA
  AGAGCTAGAGGCCCAACCAAGCCAAGCAATCTTGACTACTCCCAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR225685 representing NM_009891
  Red=Cloning site Green=Tags(s)
  
  MPILEKVPPKMPVQASSCEEVLDLPKLPVPPLQQTLATYLQCMQHLVPEEQFRKSQAIVKRFGAPGGLGE
  TLQEKLLERQEKTANWVSEYWLNDMYLNNRLALPVNSSPAVIFARQHFQDTNDQLRFAASLISGVLSYKA
  LLDSQSIPTDWAKGQLSGQPLCMKQYYRLFSSYRLPGHTQDTLVAQKSSIMPEPEHVIVACCNQFFVLDV
  VINFRRLSEGDLFTQLRKIVKMASNEDERLPPIGLLTSDGRSEWAKARTVLLKDSTNRDSLDMIERCICL
  VCLDGPGTGDLSDTHRALQLLHGGGCSLNGANRWYDKSLQFVVGRDGTCGVVCEHSPFDGIVLVQCTEHL
  LKHMMTGNKKLVRADSVSELPAPRRLRWKCSPETQGHLASSAEKLQRIVKNLDFIVYKFDNYGKTFIKKQ
  KCSPDGFIQVALQLAYYRLYQRLVPTYESASIRRFQEGRVDNIRSATPEALAFVQAMTDHKAAVLASEKL
  QLLQRAIQAQTEYTVMAITGMAIDNHLLALRELARDLCKEPPEMFMDETYLMSNRFILSTSQVPTTMEMF
  CCYGPVVPNGYGACYNPQPEAITFCISSFHGCKETSSVEFAEAVGASLVDMRDLCSSRQPAEGKPPTAKE
  RARGPTKPSNLDYSH
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_009891
• ORF Size: 1935 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_009891.1, NM_009891.2, NP_034021.1
• RefSeq Size: 2810
• RefSeq ORF: 1938
• Locus ID: 12647
• MW: 72.3 kDa