Kit (NM_001122733) Mouse Tagged ORF Clone

CAT#: MR227469

Kit (Myc-DDK-tagged) - Mouse kit oncogene (Kit), transcript variant 1

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Kit
• Synonyms: Bs; c-KIT; CD117; Fdc; Gsfsco1; Gsfsco5; Gsfsow3; SCO1; SCO5; SOW3; Ssm; Tr-kit; W
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR227469 representing NM_001122733
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGAGGCGCTCGCGGCGCCTGGGATCTGCTCTGCGTCCTGTTGGTCCTGCTCCGTGGCCAGACAGCCA
  CGTCTCAGCCATCTGCAAGTCCAGGGGAGCCGTCTCCGCCATCCATCCATCCAGCACAATCAGAGTTAAT
  AGTTGAAGCTGGCGACACCCTCAGCCTGACGTGCATTGATCCCGACTTTGTCAGATGGACTTTCAAGACC
  TATTTCAATGAAATGGTTGAGAATAAAAAAAATGAATGGATCCAGGAAAAAGCCGAGGCCACTCGCACGG
  GCACATACACGTGCAGCAACAGCAATGGCCTCACGAGTTCTATTTACGTGTTTGTTAGAGATCCTGCCAA
  ACTTTTCCTGGTTGGCCTTCCCTTGTTTGGCAAAGAAGACAGCGACGCGCTGGTCCGCTGCCCTCTGACA
  GACCCACAGGTGTCCAATTATTCCCTCATCGAGTGTGATGGGAAATCTCTCCCCACGGACCTGACGTTTG
  TCCCAAACCCCAAGGCTGGCATCACCATCAAAAACGTGAAGCGCGCCTACCACCGGCTCTGTGTCCGCTG
  TGCTGCTCAGCGTGACGGTACATGGCTGCATTCTGACAAATTCACCCTCAAAGTGCGGGCAGCCATCAAG
  GCTATCCCTGTTGTGTCTGTGCCTGAAACAAGTCACCTCCTTAAGAAAGGGGACACATTTACGGTGGTGT
  GCACCATAAAAGATGTGTCTACATCCGTGAACTCCATGTGGCTAAAGATGAACCCTCAGCCTCAGCACAT
  AGCCCAGGTAAAGCACAATAGCTGGCACCGGGGTGACTTCAATTATGAACGCCAGGAGACGCTGACTATC
  AGCTCGGCAAGAGTTGACGATTCTGGAGTGTTCATGTGTTATGCCAATAATACTTTTGGATCAGCAAATG
  TCACAACAACCTTGAAAGTAGTAGAAAAAGGATTCATCAACATCTCCCCTGTGAAGAACACTACAGTATT
  TGTAACCGATGGAGAAAACGTAGATTTGGTTGTTGAATACGAGGCCTACCCCAAACCCGAGCACCAGCAG
  TGGATATATATGAACAGGACCTCGGCTAACAAAGGGAAGGATTATGTCAAATCTGATAACAAAAGCAACA
  TCAGATATGTGAACCAACTTCGCCTGACCAGATTAAAAGGCACAGAAGGAGGCACTTATACCTTTCTGGT
  GTCCAACTCTGATGCCAGTGCTTCCGTGACATTCAACGTTTACGTGAACACAAAACCAGAAATCCTGACG
  TACGACAGGCTCATAAATGGCATGCTCCAGTGTGTGGCAGAGGGATTCCCGGAGCCCACAATAGATTGGT
  ATTTTTGTACAGGAGCAGAGCAAAGGTGTACCACTCCTGTCTCACCAGTGGACGTACAGGTCCAGAATGT
  ATCTGTGTCACCATTTGGAAAACTGGTGGTTCAGAGTTCCATAGACTCCAGCGTCTTCCGGCACAACGGC
  ACGGTGGAGTGTAAGGCCTCCAACGATGTGGGCAAGAGTTCCGCCTTCTTTAACTTTGCATTTAAAGGTA
  ACAACAAAGAGCAAATCCAGGCCCACACTCTGTTCACGCCGCTGCTCATTGGCTTTGTGGTTGCAGCTGG
  CGCGATGGGGATCATTGTGATGGTGCTCACCTACAAATATTTGCAGAAACCCATGTATGAAGTACAATGG
  AAGGTTGTCGAGGAGATAAATGGAAACAATTATGTTTACATAGACCCGACGCAACTTCCTTATGATCACA
  AATGGGAGTTTCCCAGAAACAGGCTGAGTTTTGGAAAGACATTGGGAGCTGGTGCCTTCGGGAAGGTCGT
  TGAGGCCACTGCATATGGCTTGATTAAGTCGGATGCTGCCATGACAGTTGCCGTGAAGATGCTCAAACCA
  AGTGCCCATTTAACAGAAAGAGAGGCCCTAATGTCGGAACTGAAGGTCCTGAGCTACCTGGGCAATCACA
  TGAATATTGTGAACCTGCTTGGCGCATGCACGGTGGGAGGGCCCACCCTGGTCATTACAGAATATTGTTG
  CTATGGTGATCTTTTGAATTTTTTGAGAAGGAAGCGTGACTCGTTTATTTTCTCAAAGCAAGAAGAGCAG
  GCAGAAGCGGCACTTTATAAGAACCTTCTGCACTCAACGGAGCCTTCCTGTGACAGTTCAAATGAATATA
  TGGACATGAAGCCTGGCGTTTCCTACGTGGTGCCAACCAAGACAGACAAGAGGAGATCCGCAAGAATAGA
  CTCGTACATAGAAAGAGACGTGACTCCTGCCATCATGGAAGATGACGAGCTGGCTCTGGACCTGGATGAT
  TTGCTGAGCTTCTCCTACCAGGTGGCCAAGGGCATGGCGTTCCTCGCCTCCAAGAATTGTATTCACAGAG
  ATTTGGCAGCCAGGAATATCCTCCTCACTCACGGGCGGATCACAAAGATTTGCGATTTCGGGCTAGCCAG
  AGACATCAGGAATGATTCGAATTACGTGGTCAAAGGAAATGCACGACTGCCCGTGAAGTGGATGGCACCA
  GAGAGCATTTTCAGCTGCGTGTACACATTTGAAAGTGATGTCTGGTCCTATGGGATTTTCCTCTGGGAGC
  TCTTCTCCTTAGGAAGCAGCCCCTACCCAGGGATGCCGGTCGACTCCAAGTTCTACAAGATGATCAAGGA
  AGGCTTCCGGATGGTCAGCCCGGAGCACGCGCCTGCCGAAATGTATGACGTCATGAAGACTTGCTGGGAC
  GCTGACCCCTTGAAAAGGCCAACATTCAAGCAGGTTGTCCAACTTATTGAGAAGCAGATCTCGGACAGCA
  CCAAGCACATTTACTCCAACTTGGCAAACTGCAACCCCAACCCAGAGAACCCCGTGGTGGTGGACCATTC
  CGTGAGGGTCAACTCGGTGGGCAGCAGCGCCTCTTCTACGCAGCCCCTGCTCGTGCACGAAGATGCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR227469 representing NM_001122733
  Red=Cloning site Green=Tags(s)
  
  MRGARGAWDLLCVLLVLLRGQTATSQPSASPGEPSPPSIHPAQSELIVEAGDTLSLTCIDPDFVRWTFKT
  YFNEMVENKKNEWIQEKAEATRTGTYTCSNSNGLTSSIYVFVRDPAKLFLVGLPLFGKEDSDALVRCPLT
  DPQVSNYSLIECDGKSLPTDLTFVPNPKAGITIKNVKRAYHRLCVRCAAQRDGTWLHSDKFTLKVRAAIK
  AIPVVSVPETSHLLKKGDTFTVVCTIKDVSTSVNSMWLKMNPQPQHIAQVKHNSWHRGDFNYERQETLTI
  SSARVDDSGVFMCYANNTFGSANVTTTLKVVEKGFINISPVKNTTVFVTDGENVDLVVEYEAYPKPEHQQ
  WIYMNRTSANKGKDYVKSDNKSNIRYVNQLRLTRLKGTEGGTYTFLVSNSDASASVTFNVYVNTKPEILT
  YDRLINGMLQCVAEGFPEPTIDWYFCTGAEQRCTTPVSPVDVQVQNVSVSPFGKLVVQSSIDSSVFRHNG
  TVECKASNDVGKSSAFFNFAFKGNNKEQIQAHTLFTPLLIGFVVAAGAMGIIVMVLTYKYLQKPMYEVQW
  KVVEEINGNNYVYIDPTQLPYDHKWEFPRNRLSFGKTLGAGAFGKVVEATAYGLIKSDAAMTVAVKMLKP
  SAHLTEREALMSELKVLSYLGNHMNIVNLLGACTVGGPTLVITEYCCYGDLLNFLRRKRDSFIFSKQEEQ
  AEAALYKNLLHSTEPSCDSSNEYMDMKPGVSYVVPTKTDKRRSARIDSYIERDVTPAIMEDDELALDLDD
  LLSFSYQVAKGMAFLASKNCIHRDLAARNILLTHGRITKICDFGLARDIRNDSNYVVKGNARLPVKWMAP
  ESIFSCVYTFESDVWSYGIFLWELFSLGSSPYPGMPVDSKFYKMIKEGFRMVSPEHAPAEMYDVMKTCWD
  ADPLKRPTFKQVVQLIEKQISDSTKHIYSNLANCNPNPENPVVVDHSVRVNSVGSSASSTQPLLVHEDA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001122733
• ORF Size: 2937 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001122733.1, NP_001116205.1
• RefSeq Size: 5205
• RefSeq ORF: 2940
• Locus ID: 16590
• MW: 109.8 kDa
• Gene Summary: The c-Kit proto-oncogene is the cellular homolog of the transforming gene of a feline retrovirus (v-Kit). The c-kit protein includes characteristics of a protein kinase transmembrane receptor. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]