Mtfr1 (NM_001253390) Mouse Tagged ORF Clone

CAT#: MR229265

Mtfr1 (myc-DDK-tagged) - Mouse mitochondrial fission regulator 1 (Mtfr1), transcript variant 2

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Mtfr1
• Synonyms: 1300002C08Rik; 1700080D04Rik; 2810026O10Rik; 4930579E05Rik; Chppr; Fam54a2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR229265 representing NM_001253390
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGGGCTGGATTAAGTGCCTGATGAGGATGTGGTTTCAGCGAGTTGGGGTCAGCATGCAGTCGGTAC
  TATGGTCTGGAAAACCATATGGTTCATCTCGAAGTATCGTAAGGAAAATTGGTACCAACTTATCTCTGAT
  TCAGTGTCCAAGAGTTCAATTTCAGCTTACCAGCCATGCAACAGAATGGAGTCCCACACACTCAGGAGAG
  GATGTGGTGGCATCTTTTGCTGATGTTGGATTGGTAGCCACTGAAGAAGGAGAGTGCTCAATAAGACTCA
  GGGCAGAAGTCAGTTCCAAGCCTCCTCATGAGGATGACCCTCCTTGCTTTGAGAAGCCCCCAAGCAGGCA
  CACTTCCTTCCCAAGCCTGTCTCAAGACAAGCCTTCTCCAGAGAGGACGCTGGCCAGCGAGGAGGCCCTG
  CAGAAGATCTCTGCCCTTGAGAATGAGCTTGCTGCCCTTAGAGCTCAGATTGCCAAGATTGTGACCCTGC
  AGGAGCAACAGAGTCCAAGTGCAGGTTGCTTAGATTCTTCCACATCTGTTACTGTAGCACCACCACCACC
  ACCACCTCCTCCTCCCCCTCCCCTTCCGTTGGTGCTCCACCAAAGTACGTCTGCTCTTGATCTGATTAAA
  GAAAGAAGAGAGCAGAGACTCAGTGCTGGAAAGACTTTGGCTACGGGCCACCCAAAGAAGCCTGACATGC
  CAAATATGCTTGAGATCCTTAAAGACATGAACAGTGTGAAGCTACGGTCAGTCAAGAGGTCAGAGAAAGA
  TGTAAAACCCAGGCCAGCGGATACTGACCATGCTGCCTTCATAGCAGAGGCTCTGAAAAAGAAGTTTGCA
  TATCGACACAATAGCCAAGGAGAAACTGAAAGAGGAATTCCAAAGCCAGAGTCAGAGGCCACCTCAGAGC
  CAGCCCTGTTTGGCCCACATATATTGAAGTCTACAGGGAAAATGAAGGCTTTAATTGAAAATGTTCCCGA
  CTCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR229265 representing NM_001253390
  Red=Cloning site Green=Tags(s)
  
  MLGWIKCLMRMWFQRVGVSMQSVLWSGKPYGSSRSIVRKIGTNLSLIQCPRVQFQLTSHATEWSPTHSGE
  DVVASFADVGLVATEEGECSIRLRAEVSSKPPHEDDPPCFEKPPSRHTSFPSLSQDKPSPERTLASEEAL
  QKISALENELAALRAQIAKIVTLQEQQSPSAGCLDSSTSVTVAPPPPPPPPPPPLPLVLHQSTSALDLIK
  ERREQRLSAGKTLATGHPKKPDMPNMLEILKDMNSVKLRSVKRSEKDVKPRPADTDHAAFIAEALKKKFA
  YRHNSQGETERGIPKPESEATSEPALFGPHILKSTGKMKALIENVPDS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001253390
• ORF Size: 984 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001253390.1, NP_001240319.1
• RefSeq Size: 2842 bp
• RefSeq ORF: 987 bp
• Locus ID: 67472
• MW: 36 kDa