Acidic Calponin (CNN3) (NM_001839) Human Tagged ORF Clone

CAT#: RC200488

CNN3 (Myc-DDK-tagged)-Human calponin 3, acidic (CNN3)

Product Images

Western blot validation of overexpression lysate (Cat# LY419719) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC200488 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified CNN3 protein (Cat# TP300488). The protein was produced from HEK293T cells transfected with CNN3 cDNA clone (Cat# RC200488) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CNN3
• Synonyms: calponin, acidic; calponin 3; calponin 3, acidic; dJ639P13.2.2 (acidic calponin 3)
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC200488 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACCCACTTCAACAAGGGCCCTTCCTATGGGCTCTCGGCCGAAGTCAAGAACAAGATTGCTTCCAAGT
  ATGATCATCAGGCAGAAGAAGATCTTCGCAATTGGATAGAAGAGGTGACAGGCATGAGCATTGGCCCCAA
  CTTCCAGCTGGGCTTAAAGGATGGCATCATCCTCTGCGAACTTATAAACAAGCTACAGCCAGGCTCAGTG
  AAGAAGGTCAACGAGTCCTCACTGAACTGGCCTCAGTTGGAGAATATTGGCAACTTTATTAAAGCTATTC
  AGGCTTATGGTATGAAGCCACATGACATATTCGAAGCAAATGATCTTTTTGAGAATGGAAACATGACCCA
  GGTTCAGACTACTCTGGTGGCTCTAGCAGGTCTGGCTAAAACAAAAGGATTCCATACAACCATTGACATT
  GGAGTTAAGTATGCAGAAAAACAAACAAGACGTTTTGATGAAGGAAAATTAAAAGCTGGCCAAAGTGTAA
  TTGGTCTGCAGATGGGAACCAACAAATGTGCCAGCCAGGCAGGTATGACAGCTTACGGGACTAGGAGGCA
  TCTTTATGATCCCAAAATGCAAACTGACAAACCTTTTGACCAGACCACAATTAGTCTGCAGATGGGCACT
  AATAAAGGAGCCAGCCAGGCAGGGATGTTAGCACCAGGTACCAGAAGAGACATCTATGATCAGAAGCTAA
  CATTACAGCCGGTGGACAACTCGACAATTTCCCTACAGATGGGTACCAACAAAGTTGCTTCCCAGAAAGG
  AATGAGTGTGTATGGGCTTGGGCGGCAAGTATATGATCCCAAATACTGTGCTGCTCCTACAGAACCTGTC
  ATTCACAACGGAAGCCAAGGAACAGGAACAAATGGTTCGGAAATCAGTGATAGTGATTATCAGGCAGAAT
  ACCCTGATGAGTATCATGGCGAGTACCAGGATGACTACCCCAGAGATTACCAATATAGCGACCAAGGCAT
  TGATTAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC200488 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MTHFNKGPSYGLSAEVKNKIASKYDHQAEEDLRNWIEEVTGMSIGPNFQLGLKDGIILCELINKLQPGSV
  KKVNESSLNWPQLENIGNFIKAIQAYGMKPHDIFEANDLFENGNMTQVQTTLVALAGLAKTKGFHTTIDI
  GVKYAEKQTRRFDEGKLKAGQSVIGLQMGTNKCASQAGMTAYGTRRHLYDPKMQTDKPFDQTTISLQMGT
  NKGASQAGMLAPGTRRDIYDQKLTLQPVDNSTISLQMGTNKVASQKGMSVYGLGRQVYDPKYCAAPTEPV
  IHNGSQGTGTNGSEISDSDYQAEYPDEYHGEYQDDYPRDYQYSDQGIDY
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001839
• ORF Size: 987 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001839.1, NM_001839.2, NM_001839.3, NM_001839.4, NP_001830.1
• RefSeq Size: 2112 bp
• RefSeq ORF: 990 bp
• Locus ID: 1266
• Cytogenetics: 1p21.3
• Domains: calponin, CH
• MW: 36.4 kDa
• Gene Summary: 'This gene encodes a protein with a markedly acidic C terminus; the basic N-terminus is highly homologous to the N-terminus of a related gene, CNN1. Members of the CNN gene family all contain similar tandemly repeated motifs. This encoded protein is associated with the cytoskeleton but is not involved in contraction. [provided by RefSeq, Jul 2008]'