PRCP (NM_005040) Human Tagged ORF Clone
CAT#: RC200677L4
- LentiORF®
Lenti ORF clone of Human prolylcarboxypeptidase (angiotensinase C) (PRCP), transcript variant 1, mGFP tagged
"NM_005040" in other vectors (4)
Product Images
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | mGFP |
Symbol | PRCP |
Synonyms | HUMPCP; PCP |
Vector | pLenti-C-mGFP-P2A-Puro |
E. coli Selection | Chloramphenicol (34 ug/mL) |
Mammalian Cell Selection | Puromycin |
Sequence Data |
The ORF insert of this clone is exactly the same as(RC200677).
|
Restriction Sites | SgfI-MluI Cloning Scheme for this gene |
ACCN | NM_005040 |
ORF Size | 1488 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_005040.2, NP_005031.1 |
RefSeq Size | 2161 bp |
RefSeq ORF | 1491 bp |
Locus ID | 5547 |
Cytogenetics | 11q14.1 |
Domains | abhydrolase |
Protein Families | Druggable Genome, Protease, Transmembrane |
MW | 55.8 kDa |
Gene Summary | 'This gene encodes a member of the peptidase S28 family of serine exopeptidases. The encoded preproprotein is proteolytically processed to generate the mature lysosomal prolylcarboxypeptidase. This enzyme cleaves C-terminal amino acids linked to proline in peptides such as angiotension II, III and des-Arg9-bradykinin. The cleavage occurs at acidic pH, but the enzyme activity is retained with some substrates at neutral pH. This enzyme has been shown to be an activator of the cell matrix-associated prekallikrein. The importance of angiotension II, one of the substrates of this enzyme, in regulating blood pressure and electrolyte balance suggests that this gene may be related to essential hypertension. A pseudogene of this gene has been identified on chromosome 2. Alternative splicing results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed. [provided by RefSeq, Jan 2016]' |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC116956 | PRCP (untagged)-Human prolylcarboxypeptidase (angiotensinase C) (PRCP), transcript variant 1 |
USD 310.00 |
|
RC200677 | PRCP (Myc-DDK-tagged)-Human prolylcarboxypeptidase (angiotensinase C) (PRCP), transcript variant 1 |
USD 420.00 |
|
RG200677 | PRCP (GFP-tagged) - Human prolylcarboxypeptidase (angiotensinase C) (PRCP), transcript variant 1 |
USD 460.00 |
|
RC200677L3 | Lenti ORF clone of Human prolylcarboxypeptidase (angiotensinase C) (PRCP), transcript variant 1, Myc-DDK-tagged |
USD 620.00 |
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