CCDC22 (NM_014008) Human Tagged ORF Clone

CAT#: RC201585

CCDC22 (Myc-DDK-tagged)-Human coiled-coil domain containing 22 (CCDC22)

Product Images

Western blot validation of overexpression lysate (Cat# LY415538) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC201585 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CCDC22
• Synonyms: CXorf37; JM1; RTSC2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC201585 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGAGGCGGACCGAATCCTCATCCATTCGCTGCGCCAGGCCGGCACGGCAGTTCCTCCAGATGTGC
  AGACCTTGCGCGCCTTCACCACTGAGCTGGTTGTAGAGGCTGTGGTCCGCTGCCTGCGTGTGATCAACCC
  TGCGGTGGGCTCTGGCCTCAGCCCTCTGCTGCCTCTTGCCATGTCTGCCCGGTTCCGCCTGGCCATGAGC
  CTGGCTCAGGCCTGCATGGACCTGGGCTATCCCTTGGAGCTTGGCTATCAGAACTTCCTCTACCCCAGTG
  AGCCTGACCTCCGAGACCTGCTTCTCTTCTTGGCTGAGCGTCTGCCCACCGATGCCTCTGAGGATGCAGA
  CCAGCCTGCAGGTGACTCAGCTATTCTCCTCCGGGCCATTGGGAGCCAAATTCGGGACCAGCTGGCACTG
  CCTTGGGTCCCGCCCCACCTTCGCACTCCCAAGCTGCAGCACCTCCAGGGCTCGGCCCTCCAGAAGCCTT
  TCCATGCCAGCAGGCTGGTCGTGCCAGAATTGAGTTCCAGAGGTGAGCCACGGGAGTTCCAGGCGAGTCC
  CCTGCTGCTTCCAGTCCCTACCCAGGTGCCTCAGCCTGTTGGAAGGGTGGCCTCGCTCCTCGAACACCAT
  GCCCTGCAGCTCTGCCAGCAGACGGGCCGGGACCGGCCAGGGGATGAGGACTGGGTCCACCGGACATCCC
  GCCTCCCACCCCAGGAGGACACACGGGCTCAGCGGCAGCGGCTGCAGAAGCAACTGACTGAGCATCTGCG
  CCAAAGCTGGGGCCTGCTTGGGGCCCCCATACAAGCCCGGGACCTGGGAGAACTGCTGCAGGCCTGGGGT
  GCTGGGGCCAAGACTGGTGCTCCTAAGGGCTCCCGCTTCACGCACTCAGAGAAGTTCACCTTCCATCTGG
  AGCCCCAGGCCCAGGCCACTCAGGTGTCAGATGTGCCAGCCACCTCCCGGCGGCCTGAACAGGTCACGTG
  GGCAGCTCAGGAACAGGAGCTCGAGTCCCTTCGGGAGCAGCTGGAAGGAGTGAACCGCAGCATTGAGGAG
  GTTGAGGCCGACATGAAGACCCTGGGCGTCAGCTTTGTGCAGGCAGAGTCTGAGTGCCGGCACAGCAAGC
  TCAGTACAGCAGAGCGTGAGCAGGCCCTGCGCCTGAAGAGCCGCGCGGTGGAGCTGCTGCCCGATGGGAC
  TGCCAACCTTGCCAAGCTGCAGCTTGTGGTGGAGAATAGTGCCCAGCGGGTCATCCACTTGGCGGGTCAG
  TGGGAGAAGCACCGGGTCCCACTCCTCGCTGAGTACCGCCACCTCCGAAAGCTGCAGGATTGCAGAGAGC
  TGGAATCTTCTCGACGGCTGGCAGAGATCCAAGAACTGCACCAGAGTGTCCGGGCGGCTGCTGAAGAGGC
  CCGCAGGAAGGAGGAGGTCTATAAGCAGCTGATGTCAGAGCTGGAGACTCTGCCCAGAGATGTGTCCCGG
  CTGGCCTACACCCAGCGCATCCTGGAGATCGTGGGCAACATCCGGAAGCAGAAGGAAGAGATCACCAAGA
  TCTTGTCTGATACGAAGGAGCTTCAGAAGGAAATCAACTCCCTATCTGGGAAGCTGGACCGGACGTTTGC
  GGTGACTGATGAGCTTGTGTTCAAGGATGCCAAGAAGGACGATGCTGTTCGGAAGGCCTATAAGTATCTA
  GCTGCTCTGCACGAGAACTGCAGCCAGCTCATCCAGACCATCGAGGACACAGGCACCATCATGCGGGAGG
  TTCGAGACCTCGAGGAGCAGATCGAGACAGAGCTGGGCAAGAAGACCCTCAGCAACCTGGAGAAGATCCG
  GGAGGACTACCGAGCCCTCCGCCAGGAGAACGCTGGCCTCCTAGGCCGGGTCCGGGAGGCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC201585 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MEEADRILIHSLRQAGTAVPPDVQTLRAFTTELVVEAVVRCLRVINPAVGSGLSPLLPLAMSARFRLAMS
  LAQACMDLGYPLELGYQNFLYPSEPDLRDLLLFLAERLPTDASEDADQPAGDSAILLRAIGSQIRDQLAL
  PWVPPHLRTPKLQHLQGSALQKPFHASRLVVPELSSRGEPREFQASPLLLPVPTQVPQPVGRVASLLEHH
  ALQLCQQTGRDRPGDEDWVHRTSRLPPQEDTRAQRQRLQKQLTEHLRQSWGLLGAPIQARDLGELLQAWG
  AGAKTGAPKGSRFTHSEKFTFHLEPQAQATQVSDVPATSRRPEQVTWAAQEQELESLREQLEGVNRSIEE
  VEADMKTLGVSFVQAESECRHSKLSTAEREQALRLKSRAVELLPDGTANLAKLQLVVENSAQRVIHLAGQ
  WEKHRVPLLAEYRHLRKLQDCRELESSRRLAEIQELHQSVRAAAEEARRKEEVYKQLMSELETLPRDVSR
  LAYTQRILEIVGNIRKQKEEITKILSDTKELQKEINSLSGKLDRTFAVTDELVFKDAKKDDAVRKAYKYL
  AALHENCSQLIQTIEDTGTIMREVRDLEEQIETELGKKTLSNLEKIREDYRALRQENAGLLGRVREA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_014008
• ORF Size: 1881 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014008.1, NM_014008.2, NM_014008.3, NM_014008.4, NP_054727.1
• RefSeq Size: 2333 bp
• RefSeq ORF: 1884 bp
• Locus ID: 28952
• MW: 70.8 kDa
• Gene Summary: This gene encodes a protein containing a coiled-coil domain. The encoded protein functions in the regulation of NF-kB (nuclear factor kappa-light-chain-enhancer of activated B cells) by interacting with COMMD (copper metabolism Murr1 domain-containing) proteins. The mouse orthologous protein has been shown to bind copines, which are calcium-dependent, membrane-binding proteins that may function in calcium signaling. This human gene has been identified as a novel candidate gene for syndromic X-linked intellectual disability. [provided by RefSeq, Aug 2013]