DDX41 (NM_016222) Human Tagged ORF Clone

CAT#: RC201898

DDX41 (Myc-DDK-tagged)-Human DEAD (Asp-Glu-Ala-Asp) box polypeptide 41 (DDX41)

Product Images

Western blot validation of overexpression lysate (Cat# LY402528) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC201898 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified DDX41 protein (Cat# TP301898). The protein was produced from HEK293T cells transfected with DDX41 cDNA clone (Cat# RC201898) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: DDX41
• Synonyms: ABS; MPLPF
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC201898 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGAGTCGGAACCCGAACGGAAGCGGGCTCGCACCGACGAGGTGCCTGCCGGAGGAAGCCGCTCCG
  AGGCGGAAGATGAGGACGACGAGGACTACGTGCCCTATGTGCCGTTACGGCAGCGCCGGCAGCTACTGCT
  CCAGAAGCTGCTGCAGCGAAGACGCAAGGGAGCTGCGGAGGAAGAGCAGCAGGACAGCGGTAGTGAACCC
  CGGGGAGATGAGGACGACATCCCGCTAGGCCCTCAGTCCAACGTCAGCCTCCTGGATCAGCACCAGCACC
  TTAAAGAGAAGGCTGAAGCGCGCAAAGAGTCTGCCAAGGAGAAGCAGCTGAAGGAAGAAGAGAAGATCCT
  GGAGAGTGTTGCCGAGGGCCGAGCATTGATGTCAGTGAAGGAGATGGCTAAGGGCATTACGTATGATGAC
  CCCATCAAAACCAGCTGGACTCCACCCCGTTATGTTCTGAGCATGTCTGAAGAGCGACATGAGCGCGTGC
  GGAAGAAATACCACATCCTGGTGGAGGGAGACGGTATCCCACCACCCATCAAGAGCTTCAAGGAAATGAA
  GTTTCCTGCAGCCATCCTGAGAGGCCTGAAGAAGAAAGGCATTCACCACCCAACACCCATTCAGATCCAG
  GGCATCCCCACCATTCTATCTGGCCGTGACATGATAGGCATCGCTTTCACGGGTTCAGGCAAGACACTGG
  TGTTCACGTTGCCCGTCATCATGTTCTGCCTGGAACAAGAGAAGAGGTTACCCTTCTCAAAGCGCGAGGG
  GCCCTATGGACTCATCATCTGCCCCTCGCGGGAGCTGGCCCGGCAGACCCATGGCATCCTGGAGTACTAC
  TGCCGCCTGCTGCAGGAGGACAGCTCACCACTCCTGCGCTGCGCCCTCTGCATTGGGGGCATGTCCGTGA
  AAGAGCAGATGGAGACCATCCGACACGGTGTACACATGATGGTGGCCACCCCGGGGCGCCTCATGGATTT
  GCTGCAGAAGAAGATGGTCAGCCTAGACATCTGTCGCTACCTGGCCCTGGACGAGGCTGACCGCATGATC
  GACATGGGCTTCGAGGGTGACATCCGTACCATCTTCTCCTACTTCAAGGGCCAGCGACAGACCCTGCTCT
  TCAGTGCCACCATGCCGAAGAAGATTCAGAACTTTGCTAAGAGTGCCCTTGTAAAGCCTGTGACCATCAA
  TGTGGGGCGCGCTGGGGCTGCCAGCCTGGATGTCATCCAGGAGGTAGAATATGTGAAGGAGGAGGCCAAG
  ATGGTGTACCTGCTCGAGTGCCTGCAGAAGACACCCCCGCCTGTACTCATCTTTGCAGAGAAGAAGGCAG
  ACGTGGACGCCATCCACGAGTACCTGCTGCTCAAGGGGGTTGAGGCCGTAGCCATCCATGGGGGCAAAGA
  CCAGGAGGAACGGACTAAGGCCATCGAGGCATTCCGGGAGGGCAAGAAGGATGTCCTAGTAGCCACAGAC
  GTTGCCTCCAAGGGCCTGGACTTCCCTGCCATCCAGCACGTCATCAATTATGACATGCCAGAGGAGATTG
  AGAACTATGTACACCGGATTGGCCGCACCGGGCGCTCGGGAAACACAGGCATCGCCACTACCTTCATCAA
  CAAAGCGTGTGATGAGTCAGTGCTGATGGACCTCAAAGCGCTGCTGCTAGAAGCCAAGCAGAAGGTGCCG
  CCCGTGCTGCAGGTGCTGCATTGCGGGGATGAGTCCATGCTGGACATTGGAGGAGAGCGCGGCTGTGCCT
  TCTGCGGGGGCCTGGGTCATCGGATCACTGACTGCCCCAAACTCGAGGCTATGCAGACCAAGCAGGTCAG
  CAACATCGGTCGCAAGGACTACCTGGCCCACAGCTCCATGGACTTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC201898 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MEESEPERKRARTDEVPAGGSRSEAEDEDDEDYVPYVPLRQRRQLLLQKLLQRRRKGAAEEEQQDSGSEP
  RGDEDDIPLGPQSNVSLLDQHQHLKEKAEARKESAKEKQLKEEEKILESVAEGRALMSVKEMAKGITYDD
  PIKTSWTPPRYVLSMSEERHERVRKKYHILVEGDGIPPPIKSFKEMKFPAAILRGLKKKGIHHPTPIQIQ
  GIPTILSGRDMIGIAFTGSGKTLVFTLPVIMFCLEQEKRLPFSKREGPYGLIICPSRELARQTHGILEYY
  CRLLQEDSSPLLRCALCIGGMSVKEQMETIRHGVHMMVATPGRLMDLLQKKMVSLDICRYLALDEADRMI
  DMGFEGDIRTIFSYFKGQRQTLLFSATMPKKIQNFAKSALVKPVTINVGRAGAASLDVIQEVEYVKEEAK
  MVYLLECLQKTPPPVLIFAEKKADVDAIHEYLLLKGVEAVAIHGGKDQEERTKAIEAFREGKKDVLVATD
  VASKGLDFPAIQHVINYDMPEEIENYVHRIGRTGRSGNTGIATTFINKACDESVLMDLKALLLEAKQKVP
  PVLQVLHCGDESMLDIGGERGCAFCGGLGHRITDCPKLEAMQTKQVSNIGRKDYLAHSSMDF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_016222
• ORF Size: 1866 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_016222.1, NM_016222.2, NM_016222.3, NP_057306.2
• RefSeq Size: 2118 bp
• RefSeq ORF: 1869 bp
• Locus ID: 51428
• Domains: DEAD, helicase_C, zf-CCHC
• Protein Families: Druggable Genome
• MW: 69.8 kDa
• Gene Summary: DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of the DEAD box protein family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. The protein encoded by this gene is a member of the DEAD box protein family and interacts with several spliceosomal proteins. In addition, the encoded protein may recognize the bacterial second messengers cyclic di-GMP and cyclic di-AMP, resulting in the induction of genes involved in the innate immune response. [provided by RefSeq, Jan 2017]