MTGR1 (CBFA2T2) (NM_001039709) Human Tagged ORF Clone

CAT#: RC202013

CBFA2T2 (Myc-DDK-tagged)-Human core-binding factor, runt domain, alpha subunit 2, translocated to, 2 (CBFA2T2), transcript variant 4

Product Images

Western blot validation of overexpression lysate (Cat# LY421813) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC202013 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified CBFA2T2 protein (Cat# TP302013). The protein was produced from HEK293T cells transfected with CBFA2T2 cDNA clone (Cat# RC202013) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CBFA2T2
• Synonyms: EHT; MTGR1; p85; ZMYND3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC202013 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGTTTCACCATGTTGGCCAGGCTCGTCTTGAACTCCTGACCTCAGGTGATCTGCCTGCATTGGCCT
  CCCAACGTGCTGGGATTACAGTTGGTCCTGAGAAAAGGGTGCCAGCGATGCCTGGATCGCCTGTGGAAGT
  GAAGATACAGTCCAGATCCTCACCTCCCACCATGCCACCCCTCCCACCAATAAATCCTGGAGGACCGAGG
  CCAGTGTCCTTCACTCCTACTGCATTAAGCAATGGCATCAACCATTCTCCTCCTACCCTGAATGGTGCCC
  CATCACCGCCACAGAGATTCAGCAATGGTCCTGCCTCCTCCACATCATCTGCACTCACAAATCAGCAATT
  GCCAGCCACTTGTGGTGCTCGACAACTCAGCAAGTTGAAACGCTTTCTTACCACTCTGCAACAGTTTGGC
  AATGACATCTCCCCTGAGATTGGGGAGAAGGTGCGGACTCTTGTTCTTGCACTGGTGAACTCAACAGTGA
  CAATTGAGGAATTCCACTGTAAGCTCCAAGAAGCCACAAACTTTCCCCTTCGTCCTTTTGTGATTCCATT
  TCTCAAGGCCAACCTGCCCCTGCTGCAGCGGGAACTGCTGCACTGCGCTCGGGCGGCCAAGCAGACCCCA
  TCCCAGTACCTGGCTCAGCACGAACACCTTCTGCTCAACACAAGCATTGCATCGCCTGCTGACTCGTCAG
  AGTTGCTCATGGAGGTGCACGGAAATGGGAAGAGGCCCAGTCCAGAGAGGAGAGAAGAGAATAGTTTTGA
  TAGAGACACAATTGCTCCTGAGCCTCCTGCCAAGAGAGTATGTACCATCAGCCCTGCTCCTCGGCACAGT
  CCTGCTCTCACTGTGCCCCTCATGAATCCTGGGGGCCAATTCCATCCTACCCCTCCACCTCTTCAGCATT
  ACACCTTAGAGGATATTGCAACTTCTCACCTGTATCGGGAACCCAACAAGATGCTAGAGCATCGAGAAGT
  TCGTGATAGACACCACAGTCTTGGTCTAAATGGAGGCTATCAAGATGAGTTGGTAGATCATCGTTTGACA
  GAAAGGGAATGGGCTGATGAATGGAAACATCTTGACCATGCGCTGAATTGCATTATGGAAATGGTAGAGA
  AAACAAGGCGCTCTATGGCAGTTCTGCGGCGCTGTCAGGAATCAGATCGTGAAGAACTCAACTACTGGAA
  AAGACGGTACAATGAAAACACAGAGCTGAGGAAAACGGGGACCGAGTTGGTCTCCAGGCAGCACAGCCCT
  GGGAGTGCAGATTCTCTCAGCAATGATTCTCAGAGAGAGTTCAACAGCAGGCCAGGTACAGGATACGTAC
  CTGTGGAGTTTTGGAAAAAAACAGAAGAAGCTGTGAATAAGGTGAAAATTCAGGCCATGTCAGAAGTACA
  GAAGGCCGTCGCTGAGGCAGAGCAGAAAGCCTTTGAAGTGATTGCAACAGAGAGAGCACGAATGGAGCAA
  ACCATAGCGGATGTCAAGCGGCAGGCCGCAGAGGATGCTTTCCTCGTCATCAATGAGCAAGAGGAGTCCA
  CGGAGAACTGCTGGAACTGTGGCCGCAAAGCCAGCGAGACATGCAGTGGCTGCAATATCGCGCGATACTG
  TGGCTCTTTCTGCCAGCACAAGGACTGGGAGCGGCACCACCGCCTCTGTGGTCAGAACCTGCATGGCCAG
  AGCCCCCACGGCCAGGGCCGGCCGCTGCTTCCTGTAGGCAGGGGCTCCTCTGCCAGGTCCGCCGACTGCA
  GCGTGCCCAGCCCAGCCCTCGACAAGACCTCGGCAACCACATCGCGTTCCTCAACACCTGCTTCTGTGAC
  AGCTATCGACACCAACGGACTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC202013 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MGFHHVGQARLELLTSGDLPALASQRAGITVGPEKRVPAMPGSPVEVKIQSRSSPPTMPPLPPINPGGPR
  PVSFTPTALSNGINHSPPTLNGAPSPPQRFSNGPASSTSSALTNQQLPATCGARQLSKLKRFLTTLQQFG
  NDISPEIGEKVRTLVLALVNSTVTIEEFHCKLQEATNFPLRPFVIPFLKANLPLLQRELLHCARAAKQTP
  SQYLAQHEHLLLNTSIASPADSSELLMEVHGNGKRPSPERREENSFDRDTIAPEPPAKRVCTISPAPRHS
  PALTVPLMNPGGQFHPTPPPLQHYTLEDIATSHLYREPNKMLEHREVRDRHHSLGLNGGYQDELVDHRLT
  EREWADEWKHLDHALNCIMEMVEKTRRSMAVLRRCQESDREELNYWKRRYNENTELRKTGTELVSRQHSP
  GSADSLSNDSQREFNSRPGTGYVPVEFWKKTEEAVNKVKIQAMSEVQKAVAEAEQKAFEVIATERARMEQ
  TIADVKRQAAEDAFLVINEQEESTENCWNCGRKASETCSGCNIARYCGSFCQHKDWERHHRLCGQNLHGQ
  SPHGQGRPLLPVGRGSSARSADCSVPSPALDKTSATTSRSSTPASVTAIDTNGL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001039709
• ORF Size: 1845 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq Size: 7449 bp
• RefSeq ORF: 1728 bp
• Locus ID: 9139
• Protein Families: Transcription Factors
• MW: 68 kDa
• Gene Summary: In acute myeloid leukemia, especially in the M2 subtype, the t(8;21)(q22;q22) translocation is one of the most frequent karyotypic abnormalities. The translocation produces a chimeric gene made up of the 5'-region of the RUNX1 (AML1) gene fused to the 3'-region of the CBFA2T1 (MTG8) gene. The chimeric protein is thought to associate with the nuclear corepressor/histone deacetylase complex to block hematopoietic differentiation. The protein encoded by this gene binds to the AML1-MTG8 complex and may be important in promoting leukemogenesis. Several transcript variants are thought to exist for this gene, but the full-length natures of only three have been described. [provided by RefSeq, Jul 2008]