USP5 (NM_003481) Human Tagged ORF Clone

CAT#: RC202624

USP5 (Myc-DDK-tagged)-Human ubiquitin specific peptidase 5 (isopeptidase T) (USP5), transcript variant 2

Product Images

Western blot validation of overexpression lysate (Cat# LY418652) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC202624 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified USP5 protein (Cat# TP302624). The protein was produced from HEK293T cells transfected with USP5 cDNA clone (Cat# RC202624) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: USP5
• Synonyms: ISOT
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC202624 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGAGCTGAGTGAGGAGGCGCTGCTGTCAGTATTACCGACGATCCGGGTCCCTAAGGCTGGAGACC
  GGGTCCACAAAGACGAGTGCGCCTTCTCCTTCGACACGCCGGAGTCTGAGGGGGGCCTCTACATCTGTAT
  GAACACGTTTCTGGGCTTTGGGAAACAGTATGTGGAGAGACATTTCAATAAGACCGGCCAGCGAGTCTAC
  TTGCACCTCCGGCGGACCCGGCGCCCGAAAGAGGAGGACCCTGCTACAGGCACTGGAGACCCACCCCGGA
  AGAAGCCCACGCGGCTGGCTATTGGTGTTGAAGGCGGATTTGACCTTAGCGAGGAGAAGTTTGAATTAGA
  CGAGGATGTGAAGATTGTCATTTTGCCAGATTACCTGGAGATTGCCCGGGATGGACTGGGGGGACTGCCT
  GACATTGTCAGAGATCGGGTGACCAGTGCAGTGGAGGCCCTACTGTCGGCCGACTCAGCCTCCCGCAAGC
  AGGAGGTGCAGGCATGGGATGGGGAAGTACGGCAGGTGTCTAAGCATGCCTTCAGCCTCAAGCAGTTGGA
  CAACCCTGCTCGAATCCCTCCCTGTGGCTGGAAGTGCTCCAAGTGTGACATGAGAGAGAACCTGTGGCTC
  AACCTGACTGATGGCTCCATCCTCTGTGGGCGACGCTACTTCGATGGCAGTGGGGGCAACAACCACGCTG
  TGGAGCACTACCGAGAGACAGGCTACCCGTTAGCTGTCAAGCTGGGCACCATCACCCCTGATGGAGCTGA
  CGTGTACTCATATGATGAGGATGACATGGTCCTGGACCCCAGCCTGGCTGAGCACCTGTCCCACTTCGGC
  ATCGACATGCTGAAGATGCAGAAGACAGACAAGACGATGACTGAGTTGGAGATAGACATGAACCAGCGGA
  TTGGTGAATGGGAGCTGATCCAGGAGTCAGGTGTGCCACTCAAGCCCCTGTTTGGGCCTGGCTACACAGG
  CATCCGGAACCTGGGTAACAGCTGCTACCTCAACTCTGTGGTCCAGGTGCTCTTCAGCATCCCTGACTTC
  CAGAGGAAGTATGTGGATAAGCTGGAGAAGATCTTCCAGAATGCCCCGACGGACCCTACCCAGGATTTCA
  GCACCCAGGTGGCCAAGCTGGGCCATGGCCTTCTCTCCGGGGAGTATTCCAAGCCAGTACCGGAGTCGGG
  CGATGGGGAGCGGGTGCCAGAACAGAAGGAAGTTCAAGATGGCATTGCCCCTCGGATGTTCAAGGCCCTC
  ATCGGCAAGGGCCACCCTGAATTCTCCACCAACCGGCAGCAGGATGCCCAGGAGTTCTTCCTTCACCTTA
  TCAACATGGTGGAGAGGAATTGCCGGAGCTCTGAAAATCCTAATGAAGTGTTCCGCTTCTTGGTGGAGGA
  AAAGATCAAGTGCCTGGCCACAGAGAAGGTGAAGTACACCCAGCGAGTTGACTACATCATGCAGCTGCCT
  GTGCCCATGGATGCAGCCCTTAACAAAGAGGAGCTTCTGGAGTACGAGGAGAAGAAGCGGCAAGCCGAAG
  AGGAGAAGATGGCACTGCCAGAACTGGTTCGGGCCCAGGTGCCCTTCAGCTCTTGCCTGGAGGCCTACGG
  GGCCCCTGAGCAGGTCGATGACTTCTGGAGCACGGCCCTGCAGGCCAAGTCAGTAGCTGTCAAGACCACA
  CGATTTGCCTCATTCCCTGACTACCTGGTCATCCAGATCAAGAAGTTCACCTTCGGCTTAGACTGGGTGC
  CCAAGAAACTGGATGTGTCCATCGAGATGCCAGAGGAGCTCGACATCTCCCAGTTGAGGGGCACAGGGCT
  GCAGCCCGGAGAGGAGGAGCTGCCAGACATTGCCCCACCCCTGGTCACTCCGGATGAGCCCAAAGCGCCC
  ATGCTGGATGAATCAGTCATCATCCAGCTGGTGGAGATGGGATTCCCTATGGACGCCTGCCGCAAAGCTG
  TCTACTACACGGGCAACAGCGGGGCTGAGGCCGCCATGAACTGGGTCATGTCACACATGGATGATCCAGA
  TTTTGCAAACCCCCTCATCCTGCCTGGCTCTAGTGGGCCGGGCTCCACAAGCGCAGCAGCCGACCCCCCT
  CCTGAGGACTGTGTGACCACCATTGTCTCCATGGGCTTCTCCCGGGACCAGGCCTTGAAAGCGCTGCGGG
  CCACGAACAATAGTTTAGAACGGGCTGTGGACTGGATCTTCAGTCACATTGACGACCTGGATGCTGAAGC
  TGCCATGGACATCTCAGAGGGCCGCTCAGCTGCCGACTCCATCTCTGAGTCTGTGCCAGTGGGACCTAAA
  GTCCGGGATGGTCCTGGAAAGTATCAGCTCTTTGCCTTCATTAGTCACATGGGCACCTCTACCATGTGTG
  GTCACTACGTCTGCCACATCAAGAAAGAAGGCAGATGGGTGATCTACAATGACCAGAAAGTGTGTGCCTC
  CGAGAAGCCGCCCAAGGACCTGGGCTACATCTACTTCTACCAGAGAGTGGCCAGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC202624 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAELSEEALLSVLPTIRVPKAGDRVHKDECAFSFDTPESEGGLYICMNTFLGFGKQYVERHFNKTGQRVY
  LHLRRTRRPKEEDPATGTGDPPRKKPTRLAIGVEGGFDLSEEKFELDEDVKIVILPDYLEIARDGLGGLP
  DIVRDRVTSAVEALLSADSASRKQEVQAWDGEVRQVSKHAFSLKQLDNPARIPPCGWKCSKCDMRENLWL
  NLTDGSILCGRRYFDGSGGNNHAVEHYRETGYPLAVKLGTITPDGADVYSYDEDDMVLDPSLAEHLSHFG
  IDMLKMQKTDKTMTELEIDMNQRIGEWELIQESGVPLKPLFGPGYTGIRNLGNSCYLNSVVQVLFSIPDF
  QRKYVDKLEKIFQNAPTDPTQDFSTQVAKLGHGLLSGEYSKPVPESGDGERVPEQKEVQDGIAPRMFKAL
  IGKGHPEFSTNRQQDAQEFFLHLINMVERNCRSSENPNEVFRFLVEEKIKCLATEKVKYTQRVDYIMQLP
  VPMDAALNKEELLEYEEKKRQAEEEKMALPELVRAQVPFSSCLEAYGAPEQVDDFWSTALQAKSVAVKTT
  RFASFPDYLVIQIKKFTFGLDWVPKKLDVSIEMPEELDISQLRGTGLQPGEEELPDIAPPLVTPDEPKAP
  MLDESVIIQLVEMGFPMDACRKAVYYTGNSGAEAAMNWVMSHMDDPDFANPLILPGSSGPGSTSAAADPP
  PEDCVTTIVSMGFSRDQALKALRATNNSLERAVDWIFSHIDDLDAEAAMDISEGRSAADSISESVPVGPK
  VRDGPGKYQLFAFISHMGTSTMCGHYVCHIKKEGRWVIYNDQKVCASEKPPKDLGYIYFYQRVAS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_003481
• ORF Size: 2505 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003481.1, NM_003481.2, NP_003472.1
• RefSeq Size: 3130 bp
• RefSeq ORF: 2508 bp
• Locus ID: 8078
• Domains: UBA, UCH, zf-UBP
• Protein Families: Druggable Genome, Protease
• MW: 93.3 kDa
• Gene Summary: Ubiquitin (see MIM 191339)-dependent proteolysis is a complex pathway of protein metabolism implicated in such diverse cellular functions as maintenance of chromatin structure, receptor function, and degradation of abnormal proteins. A late step of the process involves disassembly of the polyubiquitin chains on degraded proteins into ubiquitin monomers. USP5 disassembles branched polyubiquitin chains by a sequential exo mechanism, starting at the proximal end of the chain (Wilkinson et al., 1995 [PubMed 7578059]). [supplied by OMIM, Mar 2010]