CES2 (NM_003869) Human Tagged ORF Clone

CAT#: RC203009

CES2 (Myc-DDK-tagged)-Human carboxylesterase 2 (CES2), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY401274) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC203009 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified CES2 protein (Cat# TP303009). The protein was produced from HEK293T cells transfected with CES2 cDNA clone (Cat# RC203009) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CES2
• Synonyms: CE-2; CES2A1; iCE; PCE-2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC203009 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACTGCTCAGTCCCGCTCTCCTACCACACCCACCTTTCCCGGCCCAAGCCAGCGCACCCCGCTGACTC
  CCTGCCCAGTCCAAACTCCAAGGCTGGGCAAGGCACTGATCCACTGCTGGACAGACCCGGGGCAGCCTCT
  GGGTGAACAGCAGCGTGTCCGCCGGCAGCGAACCGAGACCAGCGAGCCGACCATGCGGCTGCACAGACTT
  CGTGCGCGGCTGAGCGCGGTGGCCTGTGGGCTTCTGCTGCTTCTTGTCCGGGGCCAGGGCCAGGACTCAG
  CCAGTCCCATCCGGACCACACACACGGGGCAGGTGCTGGGGAGTCTTGTCCATGTGAAGGGCGCCAATGC
  CGGGGTCCAAACCTTCCTGGGAATTCCATTTGCCAAGCCACCTCTAGGTCCGCTGCGATTTGCACCCCCT
  GAGCCCCCTGAATCTTGGAGTGGTGTGAGGGATGGAACCACCCATCCGGCCATGTGTCTACAGGACCTCA
  CCGCAGTGGAGTCAGAGTTTCTTAGCCAGTTCAACATGACCTTCCCTTCCGACTCCATGTCTGAGGACTG
  CCTGTACCTCAGCATCTACACGCCGGCCCATAGCCATGAAGGCTCTAACCTGCCGGTGATGGTGTGGATC
  CACGGTGGTGCGCTTGTTTTTGGCATGGCTTCCTTGTATGATGGTTCCATGCTGGCTGCCTTGGAGAACG
  TGGTGGTGGTCATCATCCAGTACCGCCTGGGTGTCCTGGGCTTCTTCAGCACTGGAGACAAGCACGCAAC
  CGGCAACTGGGGCTACCTGGACCAAGTGGCTGCACTACGCTGGGTCCAGCAGAATATCGCCCACTTTGGA
  GGCAACCCTGACCGTGTCACCATTTTTGGCGAGTCTGCGGGTGGCACGAGTGTGTCTTCGCTTGTTGTGT
  CCCCCATATCCCAAGGACTCTTCCACGGAGCCATCATGGAGAGTGGCGTGGCCCTCCTGCCCGGCCTCAT
  TGCCAGCTCAGCTGATGTCATCTCCACGGTGGTGGCCAACCTGTCTGCCTGTGACCAAGTTGACTCTGAG
  GCCCTGGTGGGCTGCCTGCGGGGCAAGAGTAAAGAGGAGATTCTTGCAATTAACAAGCCTTTCAAGATGA
  TCCCCGGAGTGGTGGATGGGGTCTTCCTGCCCAGGCACCCCCAGGAGCTGCTGGCCTCTGCCGACTTTCA
  GCCTGTCCCTAGCATTGTTGGTGTCAACAACAATGAATTCGGCTGGCTCATCCCCAAGGTCATGAGGATC
  TATGATACCCAGAAGGAAATGGACAGAGAGGCCTCCCAGGCTGCTCTGCAGAAAATGTTAACGCTGCTGA
  TGTTGCCTCCTACATTTGGTGACCTGCTGAGGGAGGAGTACATTGGGGACAATGGGGATCCCCAGACCCT
  CCAAGCGCAGTTCCAGGAGATGATGGCGGACTCCATGTTTGTGATCCCTGCACTCCAAGTAGCACATTTT
  CAGTGTTCCCGGGCCCCTGTGTACTTCTACGAGTTCCAGCATCAGCCCAGCTGGCTCAAGAACATCAGGC
  CACCGCACATGAAGGCAGACCATGGTGATGAGCTTCCTTTTGTTTTCAGAAGTTTCTTTGGGGGCAACTA
  CATTAAATTCACTGAGGAAGAGGAGCAGCTAAGCAGGAAGATGATGAAGTACTGGGCCAACTTTGCGAGA
  AATGGGAACCCCAATGGCGAGGGTCTGCCACACTGGCCGCTGTTCGACCAGGAGGAGCAATACCTGCAGC
  TGAACCTACAGCCTGCGGTGGGCCGGGCTCTGAAGGCCCACAGGCTCCAGTTCTGGAAGAAGGCGCTGCC
  CCAAAAGATCCAGGAGCTCGAGGAGCCTGAAGAGAGACACACAGAGCTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC203009 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MTAQSRSPTTPTFPGPSQRTPLTPCPVQTPRLGKALIHCWTDPGQPLGEQQRVRRQRTETSEPTMRLHRL
  RARLSAVACGLLLLLVRGQGQDSASPIRTTHTGQVLGSLVHVKGANAGVQTFLGIPFAKPPLGPLRFAPP
  EPPESWSGVRDGTTHPAMCLQDLTAVESEFLSQFNMTFPSDSMSEDCLYLSIYTPAHSHEGSNLPVMVWI
  HGGALVFGMASLYDGSMLAALENVVVVIIQYRLGVLGFFSTGDKHATGNWGYLDQVAALRWVQQNIAHFG
  GNPDRVTIFGESAGGTSVSSLVVSPISQGLFHGAIMESGVALLPGLIASSADVISTVVANLSACDQVDSE
  ALVGCLRGKSKEEILAINKPFKMIPGVVDGVFLPRHPQELLASADFQPVPSIVGVNNNEFGWLIPKVMRI
  YDTQKEMDREASQAALQKMLTLLMLPPTFGDLLREEYIGDNGDPQTLQAQFQEMMADSMFVIPALQVAHF
  QCSRAPVYFYEFQHQPSWLKNIRPPHMKADHGDELPFVFRSFFGGNYIKFTEEEEQLSRKMMKYWANFAR
  NGNPNGEGLPHWPLFDQEEQYLQLNLQPAVGRALKAHRLQFWKKALPQKIQELEEPEERHTEL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_003869
• ORF Size: 1869 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003869.4, NM_003869.5, NP_003860.2
• RefSeq Size: 3955 bp
• RefSeq ORF: 1680 bp
• Locus ID: 8824
• Domains: COesterase
• Protein Families: Druggable Genome
• Protein Pathways: Drug metabolism - other enzymes
• MW: 68.9 kDa
• Gene Summary: This gene encodes a member of the carboxylesterase large family. The family members are responsible for the hydrolysis or transesterification of various xenobiotics, such as cocaine and heroin, and endogenous substrates with ester, thioester, or amide bonds. They may participate in fatty acyl and cholesterol ester metabolism, and may play a role in the blood-brain barrier system. The protein encoded by this gene is the major intestinal enzyme and functions in intestine drug clearance. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Oct 2010]