CLPB (NM_030813) Human Tagged ORF Clone

CAT#: RC203013

CLPB (Myc-DDK-tagged)-Human ClpB caseinolytic peptidase B homolog (E. coli) (CLPB)

Product Images

Western blot validation of overexpression lysate (Cat# LY410684) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC203013 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CLPB
• Synonyms: ANKCLB; HSP78; MEGCANN; MGCA7; SKD3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC203013 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGGGGTCCCTGGTGTTGAGGAGAAAAGCACTGGCGCCACGGCTACTCCTCCGGCTGCTCAGGTCCC
  CAACGCTCCGGGGCCATGGAGGTGCTTCCGGCCGGAATGTGACTACTGGGAGTCTCGGGGAGCCGCAGTG
  GCTGAGGGTAGCCACCGGGGGGCGCCCTGGAACATCGCCGGCCTTGTTCTCCGGACGTGGGGCAGCCACC
  GGGGGGCGCCAGGGAGGACGCTTCGATACCAAATGCCTCGCGGCTGCCACTTGGGGACGCCTTCCTGGTC
  CCGAAGAAACACTCCCAGGACAGGACAGCTGGAACGGGGTCCCCAGCAGGGCCGGACTGGGCATGTGCGC
  CCTGGCCGCAGCGCTGGTGGTTCATTGCTACAGCAAGAGTCCGTCCAACAAGGATGCAGCCCTGTTGGAA
  GCTGCCCGTGCCAACAATATGCAAGAAGTCAGCAGGCTGTTGTCAGAAGGTGCAGATGTCAATGCAAAGC
  ACAGACTTGGCTGGACAGCACTCATGGTGGCAGCCATCAACCGAAACAACAGTGTGGTACAGGTCCTGCT
  TGCTGCTGGGGCTGATCCAAACCTTGGAGATGATTTCAGCAGTGTTTACAAGACTGCCAAGGAACAGGGA
  ATCCATTCTTTGGAAGATGGGGGACAGGACGGTGCAAGCCGGCACATCACAAACCAGTGGACAAGTGCCC
  TGGAGTTCAGGAGATGGCTAGGACTCCCCGCTGGCGTCCTGATCACCCGAGAGGATGACTTCAACAACAG
  GCTGAACAACCGCGCCAGTTTCAAGGGCTGCACGGCCTTGCACTATGCTGTTCTTGCTGATGACTACCGC
  ACTGTCAAGGAGCTGCTTGATGGAGGAGCCAACCCCCTGCAGAGGAATGAAATGGGACACACACCCTTGG
  ATTATGCCCGAGAAGGGGAAGTGATGAAGCTTCTGAGGACTTCTGAAGCCAAGTACCAAGAGAAGCAGCG
  GAAGCGTGAGGCTGAGGAGCGGCGCCGCTTCCCCCTGGAGCAGCGACTAAAGGAGCACATCATTGGCCAG
  GAGAGCGCCATCGCCACAGTGGGTGCTGCGATCCGGAGGAAGGAGAATGGCTGGTACGATGAAGAACACC
  CTCTGGTCTTCCTCTTCTTGGGATCATCTGGAATAGGAAAAACAGAGCTGGCCAAGCAGACAGCCAAATA
  TATGCACAAAGATGCTAAAAAGGGCTTCATCAGGCTGGACATGTCCGAGTTCCAGGAGCGACACGAGGTG
  GCCAAGTTTATTGGGTCTCCACCAGGCTACGTTGGCCATGAGGAGGGTGGCCAGCTGACCAAGAAGTTGA
  AGCAGTGCCCCAATGCTGTGGTGCTCTTTGATGAAGTAGACAAGGCCCATCCAGATGTGCTCACCATCAT
  GCTGCAGCTGTTTGATGAGGGCCGGCTGACAGATGGAAAAGGGAAGACCATTGATTGCAAGGACGCCATC
  TTCATCATGACCTCCAATGTGGCCAGCGACGAGATCGCACAGCACGCGCTGCAGCTGAGGCAGGAAGCTT
  TGGAGATGAGCCGTAACCGTATTGCCGAAAACCTGGGGGATGTCCAGATAAGTGACAAGATCACCATCTC
  AAAGAACTTCAAGGAGAATGTGATTCGCCCTATCCTGAAAGCTCACTTCCGGAGGGATGAGTTTCTGGGA
  CGGATCAATGAGATCGTCTACTTCCTCCCCTTCTGCCACTCGGAGCTCATCCAACTCGTCAACAAGGAAC
  TAAACTTCTGGGCCAAGAGAGCCAAGCAAAGGCACAACATCACGCTGCTCTGGGACCGCGAGGTGGCAGA
  TGTGCTGGTCGACGGCTACAATGTGCACTATGGCGCCCGCTCCATCAAACATGAGGTAGAACGCCGTGTG
  GTGAACCAGCTGGCAGCAGCCTATGAGCAGGACCTGCTGCCAGGGGGCTGTACTTTGCGCATCACGGTGG
  AGGACTCAGACAAGCAGCTACTCAAAAGCCCAGAACTGCCCTCACCCCAGGCTGAGAAGCGCCTCCCCAA
  GCTGCGTCTGGAGATCATCGACAAGGACAGCAAGACTCGCAGACTGGACATCCGGGCACCACTGCACCCT
  GAGAAGGTGTGCAACACCATC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC203013 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MLGSLVLRRKALAPRLLLRLLRSPTLRGHGGASGRNVTTGSLGEPQWLRVATGGRPGTSPALFSGRGAAT
  GGRQGGRFDTKCLAAATWGRLPGPEETLPGQDSWNGVPSRAGLGMCALAAALVVHCYSKSPSNKDAALLE
  AARANNMQEVSRLLSEGADVNAKHRLGWTALMVAAINRNNSVVQVLLAAGADPNLGDDFSSVYKTAKEQG
  IHSLEDGGQDGASRHITNQWTSALEFRRWLGLPAGVLITREDDFNNRLNNRASFKGCTALHYAVLADDYR
  TVKELLDGGANPLQRNEMGHTPLDYAREGEVMKLLRTSEAKYQEKQRKREAEERRRFPLEQRLKEHIIGQ
  ESAIATVGAAIRRKENGWYDEEHPLVFLFLGSSGIGKTELAKQTAKYMHKDAKKGFIRLDMSEFQERHEV
  AKFIGSPPGYVGHEEGGQLTKKLKQCPNAVVLFDEVDKAHPDVLTIMLQLFDEGRLTDGKGKTIDCKDAI
  FIMTSNVASDEIAQHALQLRQEALEMSRNRIAENLGDVQISDKITISKNFKENVIRPILKAHFRRDEFLG
  RINEIVYFLPFCHSELIQLVNKELNFWAKRAKQRHNITLLWDREVADVLVDGYNVHYGARSIKHEVERRV
  VNQLAAAYEQDLLPGGCTLRITVEDSDKQLLKSPELPSPQAEKRLPKLRLEIIDKDSKTRRLDIRAPLHP
  EKVCNTI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_030813
• ORF Size: 2121 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_030813.1, NM_030813.2, NM_030813.3, NM_030813.4, NM_030813.5, NP_110440.1
• RefSeq Size: 3272 bp
• RefSeq ORF: 2124 bp
• Locus ID: 81570
• MW: 78.7 kDa
• Gene Summary: This gene belongs to the ATP-ases associated with diverse cellular activities (AAA+) superfamily. Members of this superfamily form ring-shaped homo-hexamers and have highly conserved ATPase domains that are involved in various processes including DNA replication, protein degradation and reactivation of misfolded proteins. All members of this family hydrolyze ATP through their AAA+ domains and use the energy generated through ATP hydrolysis to exert mechanical force on their substrates. In addition to an AAA+ domain, the protein encoded by this gene contains a C-terminal D2 domain, which is characteristic of the AAA+ subfamily of Caseinolytic peptidases to which this protein belongs. It cooperates with Hsp70 in the disaggregation of protein aggregates. Allelic variants of this gene are associated with 3-methylglutaconic aciduria, which causes cataracts and neutropenia. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2015]