alpha Adaptin (AP2A2) (NM_012305) Human Tagged ORF Clone

CAT#: RC203018

AP2A2 (Myc-DDK-tagged)-Human adaptor-related protein complex 2, alpha 2 subunit (AP2A2)

Product Images

Western blot validation of overexpression lysate (Cat# LY415866) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC203018 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: AP2A2
• Synonyms: ADTAB; CLAPA2; HIP-9; HIP9; HYPJ
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC203018 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCGGCCGTGTCCAAGGGGGACGGGATGCGGGGCCTGGCGGTCTTCATCTCGGATATCCGCAACTGTA
  AAAGTAAAGAAGCAGAAATAAAAAGGATAAACAAGGAACTGGCAAATATCAGATCAAAATTTAAAGGTGA
  CAAGGCTCTTGATGGCTATAGTAAAAAAAAGTACGTCTGCAAGTTGCTCTTCATCTTTCTCCTTGGTCAT
  GACATTGACTTTGGACACATGGAGGCTGTGAACCTGCTGAGTTCAAACAGATACACGGAAAAGCAGATCG
  GCTACCTTTTCATCTCTGTGTTGGTGAACTCAAACAGTGAGCTGATCCGCCTGATCAACAACGCCATCAA
  GAATGACCTGGCCAGCCGCAACCCCACCTTCATGGGCCTGGCCCTGCACTGCATCGCCAGCGTGGGCAGC
  CGGGAGATGGCCGAGGCCTTCGCCGGGGAGATCCCTAAGGTCCTCGTAGCCGGAGACACTATGGACAGCG
  TGAAGCAGAGCGCGGCCCTGTGCTTGCTGCGCCTGTACAGGACGTCCCCCGATCTTGTCCCCATGGGCGA
  CTGGACATCCCGAGTGGTGCACCTGCTCAATGACCAGCACTTGGGTGTGGTAACTGCAGCCACAAGTCTG
  ATCACCACTTTAGCACAGAAGAACCCAGAAGAGTTTAAAACCTCCGTGTCTCTGGCTGTCTCTAGGCTAA
  GCAGAATCGTGACGTCTGCATCCACAGATCTCCAGGATTACACTTACTATTTTGTCCCGGCTCCCTGGCT
  GTCTGTCAAACTGCTGAGACTGCTGCAGTGCTACCCACCCCCAGACCCTGCAGTGCGAGGCCGCCTGACT
  GAGTGCCTGGAGACCATCCTGAACAAAGCCCAAGAACCGCCCAAGTCGAAGAAGGTCCAGCACTCCAACG
  CGAAGAATGCCGTGCTCTTCGAGGCCATCAGCTTAATCATTCACCATGACAGTGAGCCGAACCTGCTCGT
  CCGTGCCTGCAACCAGTTGGGCCAGTTTCTGCAGCACCGCGAGACCAACCTGCGCTACCTGGCCCTGGAG
  AGCATGTGCACGCTGGCCAGCTCTGAGTTCTCCCATGAGGCTGTCAAGACGCACATCGAGACGGTCATCA
  ACGCCCTGAAGACTGAGCGGGACGTGAGCGTGCGGCAGCGGGCCGTGGACCTCCTCTACGCCATGTGCGA
  CCGCAGCAACGCCCCACAGATCGTGGCCGAGATGCTGAGCTATCTGGAGACAGCTGACTACTCCATCCGA
  GAAGAGATTGTGCTGAAGGTCGCCATCCTGGCTGAGAAGTACGCGGTGGACTACACCTGGTATGTGGATA
  CCATCTTGAACTTGATCCGAATTGCTGGTGATTACGTGAGTGAAGAGGTGTGGTACCGAGTCATTCAGAT
  CGTCATCAACCGGGACGACGTGCAGGGCTACGCGGCCAAGACTGTGTTCGAGGCTCTTCAGGCTCCCGCG
  TGCCACGAGAACCTGGTCAAAGTGGGCGGCTACATCCTGGGGGAGTTTGGAAACTTGATAGCTGGAGACC
  CGAGATCCAGCCCGCTGATCCAGTTCCACCTGCTGCACTCCAAGTTCCACCTGTGCAGCGTCCCCACCCG
  CGCGCTGCTCCTGTCCACCTACATCAAGTTCGTGAACCTCTTCCCGGAGGTGAAGCCCACCATCCAGGAC
  GTGCTGCGCAGCGACAGCCAGCTCAGGAACGCAGACGTGGAGCTGCAGCAGCGTGCTGTGGAGTACCTGC
  GGCTCAGCACCGTGGCCAGCACCGACATTCTGGCGACCGTGCTGGAGGAGATGCCCCCATTCCCGGAGCG
  GGAGTCCTCCATCTTGGCAAAGCTCAAGAAGAAGAAGGGCCCCAGCACGGTGACAGACCTGGAGGACACC
  AAGCGGGACAGGAGTGTGGACGTGAACGGGGGTCCTGAGCCTGCCCCAGCCAGTACCAGCGCCGTGTCTA
  CGCCTTCTCCGTCGGCAGACCTGCTGGGTCTCGGGGCTGCCCCCCCTGCCCCCGCGGGCCCCCCACCCTC
  CTCCGGCGGCAGCGGGCTGCTCGTGGACGTGTTCTCAGACTCGGCCTCTGTGGTCGCGCCTCTCGCTCCT
  GGCTCCGAAGACAACTTTGCCAGGTTTGTTTGTAAAAACAATGGTGTGTTGTTTGAAAACCAGCTGCTTC
  AAATTGGACTTAAGTCTGAATTTCGGCAGAATTTAGGTCGGATGTTTATCTTTTATGGTAATAAGACCTC
  CACGCAGTTCCTAAACTTTACCCCAACACTAATCTGTTCAGACGACCTTCAGCCTAACCTGAACCTGCAG
  ACCAAGCCCGTGGACCCGACCGTGGAGGGGGGCGCGCAGGTGCAGCAGGTGGTCAACATAGAGTGCGTGT
  CCGACTTCACGGAGGCGCCAGTCCTCAACATTCAGTTCAGGTATGGGGGCACCTTCCAGAACGTGTCTGT
  GCAGCTGCCCATCACTCTCAACAAATTCTTCCAGCCGACAGAAATGGCTTCTCAGGATTTCTTTCAACGT
  TGGAAGCAGTTGAGCAATCCACAGCAGGAAGTGCAGAACATCTTCAAAGCAAAGCACCCAATGGACACAG
  AAGTCACCAAAGCCAAGATCATTGGATTTGGTTCTGCACTTCTTGAAGAAGTTGATCCTAATCCTGCGAA
  TTTCGTGGGAGCTGGAATCATCCACACGAAAACCACCCAGATTGGATGCCTGCTGCGCTTGGAGCCGAAC
  CTGCAAGCCCAGATGTACCGGCTCACGCTGCGCACAAGTAAGGAAGCCGTTTCTCAGAGATTATGTGAAT
  TGCTCTCAGCGCAGTTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC203018 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MPAVSKGDGMRGLAVFISDIRNCKSKEAEIKRINKELANIRSKFKGDKALDGYSKKKYVCKLLFIFLLGH
  DIDFGHMEAVNLLSSNRYTEKQIGYLFISVLVNSNSELIRLINNAIKNDLASRNPTFMGLALHCIASVGS
  REMAEAFAGEIPKVLVAGDTMDSVKQSAALCLLRLYRTSPDLVPMGDWTSRVVHLLNDQHLGVVTAATSL
  ITTLAQKNPEEFKTSVSLAVSRLSRIVTSASTDLQDYTYYFVPAPWLSVKLLRLLQCYPPPDPAVRGRLT
  ECLETILNKAQEPPKSKKVQHSNAKNAVLFEAISLIIHHDSEPNLLVRACNQLGQFLQHRETNLRYLALE
  SMCTLASSEFSHEAVKTHIETVINALKTERDVSVRQRAVDLLYAMCDRSNAPQIVAEMLSYLETADYSIR
  EEIVLKVAILAEKYAVDYTWYVDTILNLIRIAGDYVSEEVWYRVIQIVINRDDVQGYAAKTVFEALQAPA
  CHENLVKVGGYILGEFGNLIAGDPRSSPLIQFHLLHSKFHLCSVPTRALLLSTYIKFVNLFPEVKPTIQD
  VLRSDSQLRNADVELQQRAVEYLRLSTVASTDILATVLEEMPPFPERESSILAKLKKKKGPSTVTDLEDT
  KRDRSVDVNGGPEPAPASTSAVSTPSPSADLLGLGAAPPAPAGPPPSSGGSGLLVDVFSDSASVVAPLAP
  GSEDNFARFVCKNNGVLFENQLLQIGLKSEFRQNLGRMFIFYGNKTSTQFLNFTPTLICSDDLQPNLNLQ
  TKPVDPTVEGGAQVQQVVNIECVSDFTEAPVLNIQFRYGGTFQNVSVQLPITLNKFFQPTEMASQDFFQR
  WKQLSNPQQEVQNIFKAKHPMDTEVTKAKIIGFGSALLEEVDPNPANFVGAGIIHTKTTQIGCLLRLEPN
  LQAQMYRLTLRTSKEAVSQRLCELLSAQF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_012305
• ORF Size: 2817 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_012305.1, NM_012305.2, NM_012305.3, NP_036437.1
• RefSeq Size: 4671 bp
• RefSeq ORF: 2820 bp
• Locus ID: 161
• Cytogenetics: 11p15.5
• Protein Pathways: Endocytosis, Huntington's disease
• MW: 104 kDa
• Gene Summary: 'The protein encoded by this gene is a subunit of the AP-2 adaptor protein complex, which is involved in linking lipid and protein membrane components with the clathrin lattice. This interaction supports the formation of clathrin-coated vesicles, and the encoded subunit aids in the process by binding polyphosphoinositide-containing lipids in the cell membrane. [provided by RefSeq, Nov 2016]'