Cleavage stimulation factor 2 (CSTF2) (NM_001325) Human Tagged ORF Clone

CAT#: RC204450

CSTF2 (Myc-DDK-tagged)-Human cleavage stimulation factor, 3' pre-RNA, subunit 2, 64kDa (CSTF2)

Product Images

Western blot validation of overexpression lysate (Cat# LY400533) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC204450 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified CSTF2 protein (Cat# TP304450). The protein was produced from HEK293T cells transfected with CSTF2 cDNA clone (Cat# RC204450) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CSTF2
• Synonyms: CstF-64
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC204450 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGGTTTGACTGTGAGAGACCCAGCGGTGGATCGTTCTCTACGTTCTGTGTTCGTGGGGAACATTC
  CTTATGAAGCTACTGAAGAGCAGTTGAAGGACATCTTTTCTGAGGTTGGACCTGTTGTTAGTTTCAGATT
  GGTATACGATAGAGAGACAGGAAAGCCAAAGGGTTATGGCTTCTGTGAATACCAAGACCAAGAGACAGCA
  CTTAGTGCCATGCGGAACCTGAATGGGCGCGAATTCAGTGGGAGAGCACTTCGAGTGGACAATGCTGCCA
  GTGAAAAGAACAAAGAAGAGCTGAAGAGCCTTGGCACTGGTGCCCCTGTCATTGAGTCACCTTATGGAGA
  GACCATCAGTCCTGAGGATGCCCCTGAGTCCATTAGCAAAGCAGTTGCCAGCCTTCCACCAGAGCAGATG
  TTTGAGCTGATGAAACAAATGAAGCTCTGTGTCCAGAATAGTCCCCAGGAGGCACGGAACATGTTACTTC
  AGAACCCTCAACTGGCTTATGCTTTGCTGCAAGCACAGGTAGTGATGAGAATTGTGGATCCGGAAATTGC
  CCTGAAAATTCTGCATCGCCAGACAAATATCCCAACGCTGATTGCAGGCAACCCTCAGCCAGTCCATGGT
  GCTGGGCCTGGCTCAGGATCCAATGTGTCAATGAACCAGCAGAATCCTCAGGCCCCTCAGGCCCAGTCTT
  TGGGTGGAATGCATGTCAATGGCGCACCTCCTCTGATGCAAGCTTCTATGCAGGGTGGAGTTCCAGCACC
  AGGGCAAATGCCAGCTGCTGTCACAGGACCTGGCCCTGGTTCCTTAGCTCCTGGAGGAGGAATGCAGGCT
  CAGGTTGGAATGCCAGGAAGTGGACCAGTGTCCATGGAACGGGGGCAAGTGCCGATGCAAGACCCCAGAG
  CAGCTATGCAGCGGGGATCCTTGCCTGCGAATGTCCCAACCCCTCGAGGCTTGTTAGGAGATGCTCCGAA
  TGATCCACGGGGAGGCACTTTACTTTCTGTAACTGGAGAGGTAGAGCCTAGAGGTTACTTGGGACCACCT
  CATCAGGGTCCACCCATGCACCATGTCCCTGGCCATGAGAGCCGAGGACCACCCCCACATGAACTGAGGG
  GAGGGCCATTACCCGAGCCCAGACCTCTAATGGCAGAACCAAGAGGACCCATGCTAGATCAGAGGGGTCC
  ACCCTTGGATGGCAGAGGTGGAAGGGATCCCCGAGGAATAGATGCACGAGGGATGGAGGCCCGAGCCATG
  GAGGCAAGAGGGTTAGATGCCAGAGGATTAGAGGCCCGTGCAATGGAGGCCCGTGCGATGGAAGCTCGTG
  CAATGGAGGCCCGAGCGATGGAGGCCCGTGCAATGGAAGTCCGAGGGATGGAGGCCAGAGGCATGGATAC
  CAGAGGCCCAGTGCCTGGCCCCAGAGGACCTATACCTAGTGGAATGCAGGGTCCCAGTCCAATTAACATG
  GGGGCGGTTGTCCCCCAGGGATCCAGACAGGTCCCAGTCATGCAGGGAACAGGAATGCAAGGAGCAAGTA
  TACAGGGTGGAAGCCAGCCTGGCGGCTTTAGTCCCGGGCAGAACCAAGTCACTCCACAGGATCATGAGAA
  GGCTGCTTTGATTATGCAGGTTCTACAACTGACTGCAGACCAGATTGCCATGTTGCCTCCTGAGCAAAGG
  CAGAGTATCCTGATTTTAAAGGAACAAATACAGAAATCCACTGGAGCACCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC204450 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAGLTVRDPAVDRSLRSVFVGNIPYEATEEQLKDIFSEVGPVVSFRLVYDRETGKPKGYGFCEYQDQETA
  LSAMRNLNGREFSGRALRVDNAASEKNKEELKSLGTGAPVIESPYGETISPEDAPESISKAVASLPPEQM
  FELMKQMKLCVQNSPQEARNMLLQNPQLAYALLQAQVVMRIVDPEIALKILHRQTNIPTLIAGNPQPVHG
  AGPGSGSNVSMNQQNPQAPQAQSLGGMHVNGAPPLMQASMQGGVPAPGQMPAAVTGPGPGSLAPGGGMQA
  QVGMPGSGPVSMERGQVPMQDPRAAMQRGSLPANVPTPRGLLGDAPNDPRGGTLLSVTGEVEPRGYLGPP
  HQGPPMHHVPGHESRGPPPHELRGGPLPEPRPLMAEPRGPMLDQRGPPLDGRGGRDPRGIDARGMEARAM
  EARGLDARGLEARAMEARAMEARAMEARAMEARAMEVRGMEARGMDTRGPVPGPRGPIPSGMQGPSPINM
  GAVVPQGSRQVPVMQGTGMQGASIQGGSQPGGFSPGQNQVTPQDHEKAALIMQVLQLTADQIAMLPPEQR
  QSILILKEQIQKSTGAP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001325
• ORF Size: 1731 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001325.1, NM_001325.2, NP_001316.1
• RefSeq Size: 2027 bp
• RefSeq ORF: 1734 bp
• Locus ID: 1478
• Cytogenetics: Xq22.1
• Domains: RRM
• MW: 61 kDa
• Gene Summary: 'This gene encodes a nuclear protein with an RRM (RNA recognition motif) domain. The protein is a member of the cleavage stimulation factor (CSTF) complex that is involved in the 3' end cleavage and polyadenylation of pre-mRNAs. Specifically, this protein binds GU-rich elements within the 3'-untranslated region of mRNAs. [provided by RefSeq, Jul 2008]'