UBE2W (NM_018299) Human Tagged ORF Clone
CAT#: RC204985
UBE2W (Myc-DDK-tagged)-Human ubiquitin-conjugating enzyme E2W (putative) (UBE2W), transcript variant 2
"NM_018299" in other vectors (6)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | UBE2W |
Synonyms | UBC-16; UBC16 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC204985 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGTCAATGCAGAAACGACTACAGAAAGAACTGTTGGCTTTGCAAAATGACCCACCTCCTGGAATGA CCTTAAATGAGAAGAGTGTTCAAAATTCAATTACACAGTGGATTGTAGACATGGAAGGTGCACCAGGTAC CTTATATGAAGGGGAAAAATTTCAACTTCTATTTAAATTTAGTAGTCGATATCCTTTTGACTCTCCTCAG GTCATGTTTACTGGTGAAAATATTCCTGTTCATCCTCATGTTTATAGCAATGGTCATATCTGTTTATCCA TTCTAACAGAAGACTGGTCCCCAGCGCTCTCAGTCCAATCAGTTTGTCTTAGCATTATTAGCATGCTTTC CAGCTGCAAGGAAAAGAGACGACCACCGGATAATTCTTTTTATGTGCGAACATGTAACAAGAATCCAAAG AAAACAAAATGGTGGTATCATGATGATACTTGT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC204985 protein sequence
Red=Cloning site Green=Tags(s) MASMQKRLQKELLALQNDPPPGMTLNEKSVQNSITQWIVDMEGAPGTLYEGEKFQLLFKFSSRYPFDSPQ VMFTGENIPVHPHVYSNGHICLSILTEDWSPALSVQSVCLSIISMLSSCKEKRRPPDNSFYVRTCNKNPK KTKWWYHDDTC TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_018299 |
ORF Size | 453 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_018299.1, NM_018299.2, NM_018299.3, NP_060769.2 |
RefSeq Size | 8426 bp |
RefSeq ORF | 456 bp |
Locus ID | 55284 |
Domains | UBCc |
Protein Families | Transcription Factors |
Protein Pathways | Ubiquitin mediated proteolysis |
MW | 17.3 kDa |
Gene Summary | This gene encodes a nuclear-localized ubiquitin-conjugating enzyme (E2) that, along with ubiquitin-activating (E1) and ligating (E3) enzymes, coordinates the addition of a ubiquitin moiety to existing proteins. The encoded protein promotes the ubiquitination of Fanconi anemia complementation group proteins and may be important in the repair of DNA damage. There is a pseudogene for this gene on chromosome 1. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2012] |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC113604 | UBE2W (untagged)-Human ubiquitin-conjugating enzyme E2W (putative) (UBE2W), transcript variant 2 |
USD 310.00 |
|
RG204985 | UBE2W (GFP-tagged) - Human ubiquitin-conjugating enzyme E2W (putative) (UBE2W), transcript variant 2 |
USD 460.00 |
|
RC204985L1 | Lenti ORF clone of Human ubiquitin-conjugating enzyme E2W (putative) (UBE2W), transcript variant 2, Myc-DDK-tagged |
USD 768.00 |
|
RC204985L2 | Lenti ORF clone of Human ubiquitin-conjugating enzyme E2W (putative) (UBE2W), transcript variant 2, mGFP tagged |
USD 620.00 |
|
RC204985L3 | Lenti ORF clone of Human ubiquitin-conjugating enzyme E2W (putative) (UBE2W), transcript variant 2, Myc-DDK-tagged |
USD 620.00 |
|
RC204985L4 | Lenti ORF clone of Human ubiquitin-conjugating enzyme E2W (putative) (UBE2W), transcript variant 2, mGFP tagged |
USD 620.00 |
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