FACL4 (ACSL4) (NM_004458) Human Tagged ORF Clone

CAT#: RC205356

ACSL4 (Myc-DDK-tagged)-Human acyl-CoA synthetase long-chain family member 4 (ACSL4), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY401417) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC205356 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified ACSL4 protein (Cat# TP305356). The protein was produced from HEK293T cells transfected with ACSL4 cDNA clone (Cat# RC205356) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ACSL4
• Synonyms: ACS4; FACL4; LACS4; MRX63; MRX68
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC205356 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAAAGAGAATAAAAGCTAAGCCCACTTCAGACAAACCTGGAAGTCCATATCGCTCTGTCACACACT
  TCGACTCACTAGCTGTAATAGACATCCCTGGAGCAGATACTCTGGATAAATTATTTGACCATGCTGTATC
  CAAGTTTGGGAAGAAGGACAGCCTTGGGACCAGGGAAATCCTAAGTGAAGAAAATGAAATGCAGCCAAAT
  GGAAAAGTTTTTAAGAAGTTAATTCTTGGGAATTATAAATGGATGAACTATCTTGAAGTGAATCGCAGAG
  TGAATAACTTTGGTAGTGGACTCACTGCACTGGGACTAAAACCAAAGAACACCATTGCCATCTTCTGTGA
  GACCAGGGCCGAATGGATGATTGCAGCACAGACCTGCTTTAAGTACAACTTTCCTCTTGTGACTTTATAT
  GCCACACTTGGCAAAGAAGCAGTAGTTCATGGGCTAAATGAATCTGAGGCTTCCTATCTGATTACCAGTG
  TTGAACTTCTGGAAAGTAAACTTAAGACTGCATTGTTAGATATCAGTTGTGTTAAACATATCATTTATGT
  GGACAATAAGGCTATCAATAAAGCAGAGTACCCTGAAGGATTTGAGATTCACAGCATGCAATCAGTAGAA
  GAGTTGGGATCTAACCCAGAAAACTTGGGCATTCCTCCAAGTAGACCAACGCCTTCAGACATGGCCATTG
  TTATGTATACTAGTGGTTCTACTGGCCGACCTAAGGGAGTGATGATGCATCATAGCAATTTGATAGCTGG
  AATGACAGGCCAGTGTGAAAGAATACCTGGACTGGGACCGAAGGACACATATATTGGCTACTTGCCTTTG
  GCTCATGTGCTAGAACTGACAGCAGAGATATCTTGCTTTACCTATGGCTGCAGGATTGGATATTCTTCTC
  CGCTTACACTCTCTGACCAGTCCAGCAAAATTAAAAAAGGAAGCAAAGGAGACTGTACTGTACTGAAGCC
  CACACTTATGGCTGCTGTTCCGGAAATCATGGATAGAATTTATAAGAATGTTATGAGCAAAGTCCAAGAG
  ATGAATTATATTCAGAAAACTCTGTTCAAGATAGGGTATGATTACAAATTGGAACAGATCAAAAAGGGAT
  ATGATGCACCTCTTTGCAATCTGTTACTGTTTAAAAAGGTCAAGGCCCTGCTGGGAGGGAATGTCCGCAT
  GATGCTGTCTGGAGGGGCCCCGCTATCTCCTCAGACACACCGATTCATGAATGTCTGCTTCTGCTGCCCA
  ATTGGCCAGGGTTATGGACTGACAGAATCATGTGGTGCTGGGACAGTTACTGAAGTAACTGACTATACTA
  CTGGCAGAGTTGGAGCACCTCTTATTTGCTGTGAAATTAAGCTAAAAGACTGGCAAGAAGGCGGTTATAC
  AATTAATGACAAGCCAAACCCCAGAGGTGAAATCGTAATTGGTGGACAGAACATCTCCATGGGATATTTT
  AAAAATGAAGAGAAAACAGCAGAAGATTATTCTGTGGATGAAAATGGACAAAGGTGGTTTTGCACTGGTG
  ATATTGGAGAATTCCATCCCGATGGATGTTTACAGATTATAGATCGTAAGAAAGATCTAGTGAAGTTACA
  AGCAGGAGAGTATGTATCTCTTGGGAAAGTGGAAGCTGCACTGAAGAATTGTCCACTTATTGACAACATC
  TGTGCTTTTGCCAAAAGTGATCAGTCCTATGTGATCAGTTTTGTGGTTCCTAACCAGAAAAGGTTGACAC
  TTTTGGCACAACAGAAAGGGGTAGAAGGAACTTGGGTTGATATCTGCAATAATCCTGCTATGGAAGCTGA
  AATACTGAAAGAAATTCGAGAAGCTGCAAATGCCATGAAATTGGAGCGATTTGAAATTCCAATCAAGGTT
  CGATTAAGCCCAGAGCCATGGACCCCTGAAACTGGTTTGGTAACTGATGCTTTCAAACTGAAAAGGAAGG
  AGCTGAGGAACCATTACCTCAAAGACATTGAACGAATGTATGGGGGCAAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC205356 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAKRIKAKPTSDKPGSPYRSVTHFDSLAVIDIPGADTLDKLFDHAVSKFGKKDSLGTREILSEENEMQPN
  GKVFKKLILGNYKWMNYLEVNRRVNNFGSGLTALGLKPKNTIAIFCETRAEWMIAAQTCFKYNFPLVTLY
  ATLGKEAVVHGLNESEASYLITSVELLESKLKTALLDISCVKHIIYVDNKAINKAEYPEGFEIHSMQSVE
  ELGSNPENLGIPPSRPTPSDMAIVMYTSGSTGRPKGVMMHHSNLIAGMTGQCERIPGLGPKDTYIGYLPL
  AHVLELTAEISCFTYGCRIGYSSPLTLSDQSSKIKKGSKGDCTVLKPTLMAAVPEIMDRIYKNVMSKVQE
  MNYIQKTLFKIGYDYKLEQIKKGYDAPLCNLLLFKKVKALLGGNVRMMLSGGAPLSPQTHRFMNVCFCCP
  IGQGYGLTESCGAGTVTEVTDYTTGRVGAPLICCEIKLKDWQEGGYTINDKPNPRGEIVIGGQNISMGYF
  KNEEKTAEDYSVDENGQRWFCTGDIGEFHPDGCLQIIDRKKDLVKLQAGEYVSLGKVEAALKNCPLIDNI
  CAFAKSDQSYVISFVVPNQKRLTLLAQQKGVEGTWVDICNNPAMEAEILKEIREAANAMKLERFEIPIKV
  RLSPEPWTPETGLVTDAFKLKRKELRNHYLKDIERMYGGK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_004458
• ORF Size: 2010 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004458.1, NM_004458.2, NP_004449.1
• RefSeq Size: 5039 bp
• RefSeq ORF: 2013 bp
• Locus ID: 2182
• Domains: AMP-binding
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Adipocytokine signaling pathway, Fatty acid metabolism, Metabolic pathways, PPAR signaling pathway
• MW: 74.4 kDa
• Gene Summary: The protein encoded by this gene is an isozyme of the long-chain fatty-acid-coenzyme A ligase family. Although differing in substrate specificity, subcellular localization, and tissue distribution, all isozymes of this family convert free long-chain fatty acids into fatty acyl-CoA esters, and thereby play a key role in lipid biosynthesis and fatty acid degradation. This isozyme preferentially utilizes arachidonate as substrate. The absence of this enzyme may contribute to the cognitive disability or Alport syndrome. Alternative splicing of this gene generates multiple transcript variants. [provided by RefSeq, Jan 2016]