Endothelin A Receptor (EDNRA) (NM_001957) Human Tagged ORF Clone

CAT#: RC205385

EDNRA (Myc-DDK-tagged)-Human endothelin receptor type A (EDNRA), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY400727) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC205385 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: EDNRA
• Synonyms: ET-A; ETA; ETA-R; ETAR; ETRA; hET-AR; MFDA
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC205385 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAACCCTTTGCCTCAGGGCATCCTTTTGGCTGGCACTGGTTGGATGTGTAATCAGTGATAATCCTG
  AGAGATACAGCACAAATCTAAGCAATCATGTGGATGATTTCACCACTTTTCGTGGCACAGAGCTCAGCTT
  CCTGGTTACCACTCATCAACCCACTAATTTGGTCCTACCCAGCAATGGCTCAATGCACAACTATTGCCCA
  CAGCAGACTAAAATTACTTCAGCTTTCAAATACATTAACACTGTGATATCTTGTACTATTTTCATCGTGG
  GAATGGTGGGGAATGCAACTCTGCTCAGGATCATTTACCAGAACAAATGTATGAGGAATGGCCACAACGC
  GCTGATAGCCAGTCTTGCCCTTGGAGACCTTATCTATGTGGTCATTGATCTCCCTATCAATGTATTTAAG
  CTGCTGGCTGGGCGCTGGCCTTTTGATCACAATGACTTTGGCGTATTTCTTTGCAAGCTGTTCCCCTTTT
  TGCAGAAGTCCTCGGTGGGGATCACCGTCCTCAACCTCTGCGCTCTTAGTGTTGACAGGTACAGAGCAGT
  TGCCTCCTGGAGTCGTGTTCAGGGAATTGGGATTCCTTTGGTAACTGCCATTGAAATTGTCTCCATCTGG
  ATCCTGTCGTTTATCCTGGCCATTCCTGAAGCGATTGGCTTCGTCATGGTACCCTTTGAATATAGGGGTG
  AACAGCATAAAACCTGTATGCTCAATGCCACATCAAAATTCATGGAGTTCTACCAAGATGTAAAGGACTG
  GTGGCTCTTCGGGTTCTATTTCTGTATGCCCTTGGTGTGCACTGCGATCTTCTACACCCTCATGACTTGT
  GAGATGTTGAACAGAAGGAATGGCAGCTTGAGAATTGCCCTCAGTGAACATCTTAAGCAGCGTCGAGAAG
  TGGCAAAAACAGTTTTCTGCTTGGTTGTAATTTTTGCTCTTTGCTGGTTCCCTGTTCATTTAAGCCGTAT
  ATTGAAGAAAACTGTGTATAACGAGATGGACAAGAACCGATGTGAATTACTTAGTTTCTTACTGCTCATG
  GATTACATCGGTATTAACTTGGCAACCATGAATTCATGTATAAACCCCATAGCTCTGTATTTTGTGAGCA
  AGAAATTTAAAAATTGTTTCCAGTCATGCCTCTGCTGCTGCTGTTACCAGTCCAAAAGTCTGATGACCTC
  GGTCCCCATGAACGGAACAAGCATCCAGTGGAAGGACCACGATCAAAACAACCACAACACAGACCGGAGC
  AGCCATAAGGACAGCATGAAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC205385 protein sequence
  Red=Cloning site Green=Tags(s)
  
  METLCLRASFWLALVGCVISDNPERYSTNLSNHVDDFTTFRGTELSFLVTTHQPTNLVLPSNGSMHNYCP
  QQTKITSAFKYINTVISCTIFIVGMVGNATLLRIIYQNKCMRNGHNALIASLALGDLIYVVIDLPINVFK
  LLAGRWPFDHNDFGVFLCKLFPFLQKSSVGITVLNLCALSVDRYRAVASWSRVQGIGIPLVTAIEIVSIW
  ILSFILAIPEAIGFVMVPFEYRGEQHKTCMLNATSKFMEFYQDVKDWWLFGFYFCMPLVCTAIFYTLMTC
  EMLNRRNGSLRIALSEHLKQRREVAKTVFCLVVIFALCWFPVHLSRILKKTVYNEMDKNRCELLSFLLLM
  DYIGINLATMNSCINPIALYFVSKKFKNCFQSCLCCCCYQSKSLMTSVPMNGTSIQWKDHDQNNHNTDRS
  SHKDSMN
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001957
• ORF Size: 1281 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001957.1, NM_001957.2, NM_001957.3, NP_001948.1
• RefSeq Size: 4168 bp
• RefSeq ORF: 1284 bp
• Locus ID: 1909
• Cytogenetics: 4q31.22-q31.23
• Domains: 7tm_1
• Protein Families: Druggable Genome, GPCR, Transmembrane
• Protein Pathways: Calcium signaling pathway, Neuroactive ligand-receptor interaction, Vascular smooth muscle contraction
• MW: 48.7 kDa
• Gene Summary: 'This gene encodes the receptor for endothelin-1, a peptide that plays a role in potent and long-lasting vasoconstriction. This receptor associates with guanine-nucleotide-binding (G) proteins, and this coupling activates a phosphatidylinositol-calcium second messenger system. Polymorphisms in this gene have been linked to migraine headache resistance. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2009]'