AGXT2L1 (ETNPPL) (NM_031279) Human Tagged ORF Clone

CAT#: RC205429

AGXT2L1 (Myc-DDK-tagged)-Human alanine-glyoxylate aminotransferase 2-like 1 (AGXT2L1), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY410615) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC205429 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified ETNPPL protein (Cat# TP305429). The protein was produced from HEK293T cells transfected with ETNPPL cDNA clone (Cat# RC205429) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ETNPPL
• Synonyms: AGXT2L1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC205429 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTGCGAGCTGTACAGTAAGCGGGACACTCTGGGGCTGAGGAAGAAGCACATCGGGCCCTCATGCAAAG
  TTTTCTTTGCATCGGATCCCATCAAAATAGTGAGAGCCCAGAGGCAGTACATGTTTGATGAGAACGGTGA
  ACAGTACTTGGACTGCATCAACAATGTTGCCCATGTGGGACACTGTCACCCAGGAGTGGTCAAAGCTGCC
  CTGAAACAGATGGAACTGCTAAATACAAATTCTCGATTCCTCCACGACAACATTGTTGAGTATGCCAAAC
  GCCTTTCAGCAACTCTGCCGGAGAAACTCTCTGTTTGTTATTTTACAAATTCAGGATCCGAAGCCAACGA
  CTTAGCCTTACGCCTGGCTCGGCAGTTCAGAGGCCACCAGGATGTGATCACTCTTGACCATGCTTACCAT
  GGTCACCTATCATCCTTAATTGAGATTAGCCCATATAAGTTTCAGAAAGGAAAAGATGTCAAAAAAGAAT
  TTGTACATGTGGCACCAACTCCAGATACTTACAGAGGAAAATATAGAGAAGACCATGCAGACTCAGCCAG
  TGCTTATGCAGATGAAGTGAAGAAAATCATTGAAGATGCTCATAACAGTGGAAGGAAGATTGCTGCCTTT
  ATTGCTGAATCCATGCAGAGTTGTGGCGGACAAATAATTCCTCCAGCAGGCTACTTCCAGAAAGTGGCAG
  AATATGTACACGGTGCAGGGGGTGTGTTTATAGCTGATGAAGTTCAAGTGGGCTTTGGCAGAGTTGGGAA
  ACATTTCTGGAGCTTCCAGATGTATGGTGAAGACTTTGTTCCAGACATCGTCACAATGGGAAAACCGATG
  GGCAACGGCCACCCGGTGGCATGTGTGGTAACAACCAAAGAAATTGCAGAAGCCTTCAGCAGCTCTGGGA
  TGGAATATTTTAATACGTATGGAGGAAATCCAGTATCTTGTGCTGTTGGTTTGGCTGTCCTGGATATAAT
  TGAAAATGAAGACCTTCAAGGAAATGCCAAGAGAGTAGGGAATTATCTCACTGAGTTACTGAAAAAACAG
  AAGGCTAAACACACTTTGATAGGAGATATTAGGGGCATTGGCCTTTTTATTGGAATTGATTTAGTGAAGG
  ACCATCTGAAAAGGACCCCTGCCACAGCTGAAGCTCAGCACATCATCTACAAGATGAAAGAAAAACGAGT
  GCTTCTCAGTGCCGATGGACCTCATAGAAATGTACTTAAAATAAAACCACCTATGTGCTTCACTGAAGAA
  GATGCAAAGTTCATGGTGGACCAACTTGATAGGATTCTAACAGTTTTAGAAGAAGCTATGGGAACCAAAA
  CCGAAAGTGTGACCTCTGAGAATACTCCATGCAAAACAAAGATGCTGAAAGAAGCCCACATAGAACTGCT
  TAGGGACAGCACCACTGACTCCAAAGAAAATCCCAGCAGAAAGAGAAATGGAATGTGCACGGATACACAT
  TCACTGCTCAGTAAGAGGCTCAAGACA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC205429 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MCELYSKRDTLGLRKKHIGPSCKVFFASDPIKIVRAQRQYMFDENGEQYLDCINNVAHVGHCHPGVVKAA
  LKQMELLNTNSRFLHDNIVEYAKRLSATLPEKLSVCYFTNSGSEANDLALRLARQFRGHQDVITLDHAYH
  GHLSSLIEISPYKFQKGKDVKKEFVHVAPTPDTYRGKYREDHADSASAYADEVKKIIEDAHNSGRKIAAF
  IAESMQSCGGQIIPPAGYFQKVAEYVHGAGGVFIADEVQVGFGRVGKHFWSFQMYGEDFVPDIVTMGKPM
  GNGHPVACVVTTKEIAEAFSSSGMEYFNTYGGNPVSCAVGLAVLDIIENEDLQGNAKRVGNYLTELLKKQ
  KAKHTLIGDIRGIGLFIGIDLVKDHLKRTPATAEAQHIIYKMKEKRVLLSADGPHRNVLKIKPPMCFTEE
  DAKFMVDQLDRILTVLEEAMGTKTESVTSENTPCKTKMLKEAHIELLRDSTTDSKENPSRKRNGMCTDTH
  SLLSKRLKT
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_031279
• ORF Size: 1497 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_031279.2, NM_031279.3, NP_112569.2
• RefSeq Size: 2133 bp
• RefSeq ORF: 1500 bp
• Locus ID: 64850
• Domains: aminotran_3
• Protein Families: Druggable Genome
• MW: 55.7 kDa