DYRK1B (NM_004714) Human Tagged ORF Clone

CAT#: RC205861

DYRK1B (Myc-DDK-tagged)-Human dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 1B (DYRK1B), transcript variant a

Product Images

Western blot validation of overexpression lysate (Cat# LY401491) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC205861 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: DYRK1B
• Synonyms: AOMS3; MIRK
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC205861 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCGTCCCACCGGGCCATGGTCCCTTCTCTGGCTTCCCAGGGCCCCAGGAGCACACGCAGGTATTGC
  CTGATGTGCGGCTACTGCCTCGGAGGCTGCCCCTGGCCTTCCGGGATGCAACCTCAGCCCCGCTGCGTAA
  GCTCTCTGTGGACCTCATCAAGACCTACAAGCACATCAATGAGGTATACTATGCGAAGAAGAAGCGGCGG
  GCCCAGCAGGCGCCACCCCAGGATTCGAGCAACAAGAAGGAGAAGAAGGTCCTGAACCATGGTTATGATG
  ACGACAACCATGACTACATCGTGCGCAGTGGCGAGCGCTGGCTGGAGCGCTACGAAATTGACTCGCTCAT
  TGGCAAAGGCTCCTTTGGCCAGGTGGTGAAAGCCTATGATCATCAGACCCAGGAGCTTGTGGCCATCAAG
  ATCATCAAGAACAAAAAGGCTTTCCTGAACCAGGCCCAGATTGAGCTGCGGCTGCTGGAGCTGATGAACC
  AGCATGACACGGAGATGAAGTACTATATAGTACACCTGAAGCGGCACTTCATGTTCCGGAACCACCTGTG
  CCTGGTATTTGAGCTGCTGTCCTACAACCTGTACGACCTCCTGCGCAACACCCACTTCCGCGGCGTCTCG
  CTGAACCTGACCCGGAAGCTGGCGCAGCAGCTCTGCACGGCACTGCTCTTTCTGGCCACGCCTGAGCTCA
  GCATCATTCACTGCGACCTCAAGCCCGAAAACATCTTGCTGTGCAACCCCAAGCGCAGCGCCATCAAGAT
  TGTGGACTTCGGCAGCTCCTGCCAGCTTGGCCAGAGGATCTACCAGTATATCCAGAGCCGCTTCTACCGC
  TCACCTGAGGTGCTCCTGGGCACACCCTACGACCTGGCCATTGACATGTGGTCCCTGGGCTGCATCCTTG
  TGGAGATGCACACCGGAGAGCCCCTCTTCAGTGGCTCCAATGAGGTCGACCAGATGAACCGCATTGTGGA
  GGTGCTGGGCATCCCACCGGCCGCCATGCTGGACCAGGCGCCCAAGGCTCGCAAGTACTTTGAACGGCTG
  CCTGGGGGTGGCTGGACCCTACGAAGGACGAAAGAACTCAGGAAGGATTACCAGGGCCCCGGGACACGGC
  GGCTGCAGGAGGTGCTGGGCGTGCAGACGGGCGGGCCCGGGGGCCGGCGGGCGGGGGAGCCGGGCCACAG
  CCCCGCCGACTACCTCCGCTTCCAGGACCTGGTGCTGCGCATGCTGGAGTATGAGCCCGCCGCCCGCATC
  AGCCCCCTGGGGGCTCTGCAGCACGGCTTCTTCCGCCGCACGGCCGACGAGGCCACCAACACGGGCCCGG
  CAGGCAGCAGTGCCTCCACCTCGCCCGCGCCCCTCGACACCTGCCCCTCTTCCAGCACCGCCAGCTCCAT
  CTCCAGTTCTGGAGGCTCCAGTGGCTCCTCCAGTGACAACCGGACCTACCGCTACAGCAACCGATATTGT
  GGGGGCCCTGGGCCCCCTATCACAGACTGTGAGATGAACAGCCCCCAGGTCCCACCCTCCCAGCCGCTGC
  GGCCCTGGGCAGGGGGTGATGTGCCCCACAAGACACATCAAGCCCCTGCCTCTGCCTCGTCACTGCCTGG
  GACCGGGGCCCAGTTACCCCCCCAGCCCCGATACCTTGGTCGTCCCCCATCACCAACCTCACCACCACCC
  CCGGAGCTGATGGATGTGAGCCTGGTGGGCGGCCCTGCTGACTGCTCCCCACCTCACCCAGCGCCTGCCC
  CCCAGCACCCGGCTGCCTCAGCCCTCCGGACTCGGATGACTGGAGGTCGTCCACCCCTCCCGCCTCCTGA
  TGACCCTGCCACTCTGGGGCCTCACCTGGGCCTCCGTGGTGTACCCCAGAGCACAGCAGCCAGCTCG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC205861 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAVPPGHGPFSGFPGPQEHTQVLPDVRLLPRRLPLAFRDATSAPLRKLSVDLIKTYKHINEVYYAKKKRR
  AQQAPPQDSSNKKEKKVLNHGYDDDNHDYIVRSGERWLERYEIDSLIGKGSFGQVVKAYDHQTQELVAIK
  IIKNKKAFLNQAQIELRLLELMNQHDTEMKYYIVHLKRHFMFRNHLCLVFELLSYNLYDLLRNTHFRGVS
  LNLTRKLAQQLCTALLFLATPELSIIHCDLKPENILLCNPKRSAIKIVDFGSSCQLGQRIYQYIQSRFYR
  SPEVLLGTPYDLAIDMWSLGCILVEMHTGEPLFSGSNEVDQMNRIVEVLGIPPAAMLDQAPKARKYFERL
  PGGGWTLRRTKELRKDYQGPGTRRLQEVLGVQTGGPGGRRAGEPGHSPADYLRFQDLVLRMLEYEPAARI
  SPLGALQHGFFRRTADEATNTGPAGSSASTSPAPLDTCPSSSTASSISSSGGSSGSSSDNRTYRYSNRYC
  GGPGPPITDCEMNSPQVPPSQPLRPWAGGDVPHKTHQAPASASSLPGTGAQLPPQPRYLGRPPSPTSPPP
  PELMDVSLVGGPADCSPPHPAPAPQHPAASALRTRMTGGRPPLPPPDDPATLGPHLGLRGVPQSTAASS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_004714
• ORF Size: 1887 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004714.1, NM_004714.2, NP_004705.1
• RefSeq Size: 2588 bp
• RefSeq ORF: 1890 bp
• Locus ID: 9149
• Protein Families: Druggable Genome, Protein Kinase, Transcription Factors
• MW: 69.2 kDa
• Gene Summary: This gene encodes a member of a family of nuclear-localized protein kinases. The encoded protein participates in the regulation of the cell cycle. Expression of this gene may be altered in tumor cells, and mutations in this gene were found to cause abdominal obesity-metabolic syndrome 3. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2014]