Aromatase (CYP19A1) (NM_000103) Human Tagged ORF Clone

CAT#: RC205890

CYP19A1 (Myc-DDK-tagged)-Human cytochrome P450, family 19, subfamily A, polypeptide 1 (CYP19A1), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY410595) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC221358 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified CYP19A1 protein (Cat# TP305890). The protein was produced from HEK293T cells transfected with CYP19A1 cDNA clone (Cat# RC205890) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CYP19A1
• Synonyms: ARO; ARO1; CPV1; CYAR; CYP19; CYPXIX; P-450AROM
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC205890 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTTTTGGAAATGCTGAACCCGATACATTATAACATCACCAGCATCGTGCCTGAAGCCATGCCTGCTG
  CCACCATGCCAGTCCTGCTCCTCACTGGCCTTTTTCTCTTGGTGTGGAATTATGAGGGCACATCCTCAAT
  ACCAGGTCCTGGCTACTGCATGGGAATTGGACCCCTCATCTCCCACGGCAGATTCCTGTGGATGGGGATC
  GGCAGTGCCTGCAACTACTACAACCGGGTGTATGGAGAATTCATGCGAGTCTGGATCTCTGGAGAGGAAA
  CACTCATTATCAGCAAGTCCTCAAGTATGTTCCACATAATGAAGCACAATCATTACAGCTCTCGATTCGG
  CAGCAAACTTGGGCTGCAGTGCATCGGTATGCATGAGAAAGGCATCATATTTAACAACAATCCAGAGCTC
  TGGAAAACAACTCGACCCTTCTTTATGAAAGCTCTGTCAGGCCCCGGCCTTGTTCGTATGGTCACAGTCT
  GTGCTGAATCCCTCAAAACACATCTGGACAGGTTGGAGGAGGTGACCAATGAATCGGGCTATGTGGACGT
  GTTGACCCTTCTGCGTCGTGTCATGCTGGACACCTCTAACACGCTCTTCTTGAGGATCTCTTTGGACGAA
  AGTGCTATCGTGGTTAAAATCCAAGGTTATTTTGATGCATGGCAAGCTCTCCTCATCAAACCAGACATCT
  TCTTTAAGATTTCTTGGCTATACAAAAAGTATGAGAAGTCTGTCAAGGATTTGAAAGATGCCATAGAAGT
  TCTGATAGCAGAAAAAAGACGCAGGATTTCCACAGAAGAGAAACTGGAAGAATGTATGGACTTTGCCACT
  GAGTTGATTTTAGCAGAGAAACGTGGTGACCTGACAAGAGAGAATGTGAACCAGTGCATATTGGAAATGC
  TGATCGCAGCTCCTGACACCATGTCTGTCTCTTTGTTCTTCATGCTATTTCTCATTGCAAAGCACCCTAA
  TGTTGAAGAGGCAATAATAAAGGAAATCCAGACTGTTATTGGTGAGAGAGACATAAAGATTGATGATATA
  CAAAAATTAAAAGTGATGGAAAACTTCATTTATGAGAGCATGCGGTACCAGCCTGTCGTGGACTTGGTCA
  TGCGCAAAGCCTTAGAAGATGATGTAATCGATGGCTACCCAGTGAAAAAGGGGACAAACATTATCCTGAA
  TATTGGAAGGATGCACAGACTCGAGTTTTTCCCCAAACCCAATGAATTTACTCTTGAAAATTTTGCAAAG
  AATGTTCCTTATAGGTACTTTCAGCCATTTGGCTTTGGGCCCCGTGGCTGTGCAGGAAAGTACATCGCCA
  TGGTGATGATGAAAGCCATCCTCGTTACACTTCTGAGACGATTCCACGTGAAGACATTGCAAGGACAGTG
  TGTTGAGAGCATACAGAAGATACACGACTTGTCCTTGCACCCAGATGAGACTAAAAACATGCTGGAAATG
  ATCTTTACCCCAAGAAACTCAGACAGGTGTCTGGAACAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC205890 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MVLEMLNPIHYNITSIVPEAMPAATMPVLLLTGLFLLVWNYEGTSSIPGPGYCMGIGPLISHGRFLWMGI
  GSACNYYNRVYGEFMRVWISGEETLIISKSSSMFHIMKHNHYSSRFGSKLGLQCIGMHEKGIIFNNNPEL
  WKTTRPFFMKALSGPGLVRMVTVCAESLKTHLDRLEEVTNESGYVDVLTLLRRVMLDTSNTLFLRISLDE
  SAIVVKIQGYFDAWQALLIKPDIFFKISWLYKKYEKSVKDLKDAIEVLIAEKRRRISTEEKLEECMDFAT
  ELILAEKRGDLTRENVNQCILEMLIAAPDTMSVSLFFMLFLIAKHPNVEEAIIKEIQTVIGERDIKIDDI
  QKLKVMENFIYESMRYQPVVDLVMRKALEDDVIDGYPVKKGTNIILNIGRMHRLEFFPKPNEFTLENFAK
  NVPYRYFQPFGFGPRGCAGKYIAMVMMKAILVTLLRRFHVKTLQGQCVESIQKIHDLSLHPDETKNMLEM
  IFTPRNSDRCLEH
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_000103
• ORF Size: 1509 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000103.1, NM_000103.2, NM_000103.3, NP_000094.2
• RefSeq Size: 4422 bp
• RefSeq ORF: 1512 bp
• Locus ID: 1588
• Cytogenetics: 15q21.2
• Domains: p450
• Protein Families: Druggable Genome, P450
• Protein Pathways: Androgen and estrogen metabolism, Metabolic pathways
• MW: 57.9 kDa
• Gene Summary: 'This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and catalyzes the last steps of estrogen biosynthesis. Mutations in this gene can result in either increased or decreased aromatase activity; the associated phenotypes suggest that estrogen functions both as a sex steroid hormone and in growth or differentiation. Alternative promoter use and alternative splicing results in multiple transcript variants that have different tissue specificities. [provided by RefSeq, Dec 2016]'