beta COP (COPB1) (NM_016451) Human Tagged ORF Clone

CAT#: RC206009

COPB1 (Myc-DDK-tagged)-Human coatomer protein complex, subunit beta 1 (COPB1), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY428489) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC227862 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified COPB1 protein (Cat# TP306009). The protein was produced from HEK293T cells transfected with COPB1 cDNA clone (Cat# RC206009) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: COPB1
• Synonyms: COPB
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC206009 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACGGCGGCTGAGAACGTATGCTACACGTTAATTAACGTGCCAATGGATTCAGAACCACCATCTGAAA
  TTAGCTTAAAAAATGATCTAGAAAAAGGAGATGTAAAGTCAAAGACTGAAGCTTTGAAGAAAGTAATCAT
  TATGATTCTGAATGGTGAAAAACTTCCTGGACTTCTGATGACCATCATTCGTTTTGTGCTACCTCTTCAG
  GATCACACTATCAAGAAATTACTTCTGGTATTTTGGGAAATTGTTCCTAAAACAACTCCAGATGGGAGAC
  TTTTACATGAGATGATCCTTGTATGTGATGCATACAGAAAGGATCTTCAACATCCTAATGAATTTATTCG
  AGGATCTACTCTTCGTTTTCTTTGCAAATTGAAAGAAGCAGAATTGCTAGAACCTTTAATGCCAGCTATT
  CGTGCATGTTTGGAGCATCGACACAGCTATGTTAGAAGAAATGCTGTTTTGGCCATCTATACCATCTATA
  GAAATTTTGAACATCTTATACCTGATGCTCCTGAACTGATACATGATTTTCTGGTGAATGAGAAGGATGC
  AAGTTGCAAAAGGAATGCATTTATGATGCTAATTCATGCAGATCAGGATCGAGCTTTGGATTACTTAAGT
  ACTTGCATTGATCAAGTTCAAACATTTGGAGACATTCTGCAGCTGGTTATTGTTGAACTGATTTATAAGG
  TCTGTCATGCTAATCCATCAGAAAGAGCTCGTTTTATTCGCTGCATCTATAACTTATTACAGTCATCCAG
  CCCTGCTGTAAAATATGAAGCTGCTGGGACATTAGTGACACTCTCTAGTGCACCAACTGCAATCAAGGCT
  GCTGCTCAGTGTTACATTGATTTAATTATTAAGGAGAGCGACAACAATGTAAAACTCATAGTTTTGGATC
  GCTTGATAGAATTAAAAGAGCATCCTGCTCATGAACGAGTACTACAGGATCTGGTTATGGATATCCTAAG
  AGTATTGAGCACACCAGACTTAGAAGTACGAAAGAAAACTCTGCAGTTAGCACTGGATCTTGTCTCTTCT
  AGAAATGTTGAAGAGCTGGTTATTGTCCTGAAGAAGGAAGTGATAAAAACAAATAATGTGTCTGAGCATG
  AAGATACTGACAAATACAGACAACTCCTAGTGCGAACATTGCATTCCTGTTCTGTCCGATTTCCAGATAT
  GGCTGCAAATGTTATTCCTGTGTTAATGGAATTTCTCAGTGACAACAACGAAGCAGCAGCTGCTGATGTC
  TTGGAGTTTGTTCGTGAAGCCATTCAGCGCTTTGATAACCTGAGAATGCTTATTGTTGAGAAGATGCTTG
  AAGTCTTTCATGCTATTAAATCTGTCAAGATTTACCGAGGAGCATTATGGATCCTGGGAGAATACTGTAG
  TACCAAGGAAGACATTCAGAGTGTGATGACTGAGATCCGCAGGTCCCTTGGAGAGATCCCAATTGTAGAG
  TCAGAAATAAAGAAAGAAGCTGGTGAATTAAAACCTGAAGAAGAAATAACTGTAGGGCCAGTTCAGAAAT
  TGGTTACTGAAATGGGTACCTATGCAACTCAGAGTGCCCTTAGCAGTTCTAGACCCACCAAGAAAGAGGA
  AGACAGACCTCCCTTGAGAGGATTCCTTCTGGATGGAGATTTCTTTGTTGCTGCCTCCCTTGCCACAACT
  CTGACCAAGATTGCATTGCGCTATGTAGCTTTGGTTCAGGAGAAGAAAAAGCAAAATTCTTTTGTTGCTG
  AGGCTATGTTGCTCATGGCTACTATCCTGCATTTGGGAAAATCCTCTCTTCCTAAGAAGCCAATTACTGA
  TGATGATGTGGATCGAATTTCCCTGTGCCTCAAGGTCTTGTCTGAATGTTCACCTTTAATGAATGACATT
  TTCAATAAGGAATGCAGACAGTCCCTTTCTCACATGTTATCTGCTAAACTAGAAGAAGAGAAATTATCCC
  AAAAGAAAGAATCTGAAAAGAGGAATGTGACAGTACAGCCTGATGACCCCATTTCCTTCATGCAACTAAC
  TGCTAAGAATGAAATGAACTGCAAGGAAGATCAGTTTCAGCTGAGTTTACTGGCAGCAATGGGTAACACA
  CAGAGGAAAGAGGCAGCAGATCCCCTAGCATCTAAACTTAACAAGGTCACCCAATTGACAGGTTTCTCAG
  ATCCTGTATATGCAGAAGCTTACGTTCATGTCAACCAATATGATATTGTCCTGGATGTACTTGTTGTGAA
  CCAAACCAGTGATACTTTGCAGAATTGCACATTAGAACTAGCTACACTAGGGGATCTGAAACTTGTGGAA
  AAGCCGTCTCCTTTGACTCTTGCTCCTCATGACTTCGCAAATATTAAAGCTAACGTCAAAGTAGCATCAA
  CAGAAAATGGAATAATTTTTGGTAATATAGTTTATGATGTCTCTGGAGCAGCAAGTGACAGAAATTGTGT
  GGTTCTCAGTGATATTCACATCGACATCATGGACTATATCCAGCCTGCAACTTGCACTGATGCAGAATTC
  CGTCAGATGTGGGCCGAATTTGAATGGGAAAACAAAGTGACAGTTAACACCAACATGGTTGATTTAAATG
  ACTACTTACAGCACATATTAAAGTCAACCAATATGAAATGCCTGACTCCAGAAAAGGCCCTTTCTGGTTA
  CTGTGGCTTTATGGCAGCCAACCTTTATGCTCGTTCCATATTTGGTGAAGATGCACTTGCAAATGTCAGC
  ATTGAGAAGCCAATTCACCAGGGACCAGATGCTGCTGTTACCGGCCATATAAGAATTCGTGCAAAGAGCC
  AGGGAATGGCCTTAAGTCTTGGAGATAAAATCAACTTGTCACAGAAGAAAACTAGTATA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC206009 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MTAAENVCYTLINVPMDSEPPSEISLKNDLEKGDVKSKTEALKKVIIMILNGEKLPGLLMTIIRFVLPLQ
  DHTIKKLLLVFWEIVPKTTPDGRLLHEMILVCDAYRKDLQHPNEFIRGSTLRFLCKLKEAELLEPLMPAI
  RACLEHRHSYVRRNAVLAIYTIYRNFEHLIPDAPELIHDFLVNEKDASCKRNAFMMLIHADQDRALDYLS
  TCIDQVQTFGDILQLVIVELIYKVCHANPSERARFIRCIYNLLQSSSPAVKYEAAGTLVTLSSAPTAIKA
  AAQCYIDLIIKESDNNVKLIVLDRLIELKEHPAHERVLQDLVMDILRVLSTPDLEVRKKTLQLALDLVSS
  RNVEELVIVLKKEVIKTNNVSEHEDTDKYRQLLVRTLHSCSVRFPDMAANVIPVLMEFLSDNNEAAAADV
  LEFVREAIQRFDNLRMLIVEKMLEVFHAIKSVKIYRGALWILGEYCSTKEDIQSVMTEIRRSLGEIPIVE
  SEIKKEAGELKPEEEITVGPVQKLVTEMGTYATQSALSSSRPTKKEEDRPPLRGFLLDGDFFVAASLATT
  LTKIALRYVALVQEKKKQNSFVAEAMLLMATILHLGKSSLPKKPITDDDVDRISLCLKVLSECSPLMNDI
  FNKECRQSLSHMLSAKLEEEKLSQKKESEKRNVTVQPDDPISFMQLTAKNEMNCKEDQFQLSLLAAMGNT
  QRKEAADPLASKLNKVTQLTGFSDPVYAEAYVHVNQYDIVLDVLVVNQTSDTLQNCTLELATLGDLKLVE
  KPSPLTLAPHDFANIKANVKVASTENGIIFGNIVYDVSGAASDRNCVVLSDIHIDIMDYIQPATCTDAEF
  RQMWAEFEWENKVTVNTNMVDLNDYLQHILKSTNMKCLTPEKALSGYCGFMAANLYARSIFGEDALANVS
  IEKPIHQGPDAAVTGHIRIRAKSQGMALSLGDKINLSQKKTSI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_016451
• ORF Size: 2859 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_016451.1, NM_016451.2, NM_016451.3, NM_016451.4, NP_057535.1
• RefSeq Size: 3490 bp
• RefSeq ORF: 2862 bp
• Locus ID: 1315
• Cytogenetics: 11p15.2
• Domains: Adaptin_N
• MW: 107.1 kDa
• Gene Summary: 'This gene encodes a protein subunit of the coatomer complex associated with non-clathrin coated vesicles. The coatomer complex, also known as the coat protein complex 1, forms in the cytoplasm and is recruited to the Golgi by activated guanosine triphosphatases. Once at the Golgi membrane, the coatomer complex may assist in the movement of protein and lipid components back to the endoplasmic reticulum. Alternatively spliced transcript variants have been described. [provided by RefSeq, Jan 2009]'