ALOX15 (NM_001140) Human Tagged ORF Clone

CAT#: RC206621

ALOX15 (Myc-DDK-tagged)-Human arachidonate 15-lipoxygenase (ALOX15)

Product Images

Western blot validation of overexpression lysate (Cat# LY400456) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC206621 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified ALOX15 protein (Cat# TP306621). The protein was produced from HEK293T cells transfected with ALOX15 cDNA clone (Cat# RC206621) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ALOX15
• Synonyms: 12-LOX; 15-LOX; 15-LOX-1; LOG15
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC206621 representing NM_001140
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGTCTCTACCGCATCCGCGTGTCCACTGGGGCCTCGCTCTATGCCGGTTCCAACAACCAGGTGCAGC
  TGTGGCTGGTCGGCCAGCACGGGGAGGCGGCGCTCGGGAAGCGACTGTGGCCCGCACGGGGCAAGGAGAC
  AGAACTCAAGGTGGAAGTACCGGAGTATCTGGGGCCGCTGCTGTTTGTGAAACTGCGCAAACGGCACCTC
  CTTAAGGACGACGCCTGGTTCTGCAACTGGATCTCTGTGCAGGGCCCCGGAGCCGGGGACGAGGTCAGGT
  TCCCTTGTTACCGCTGGGTGGAGGGCAACGGCGTCCTGAGCCTGCCTGAAGGCACCGGCCGCACTGTGGG
  CGAGGACCCTCAGGGCCTGTTCCAGAAACACCGGGAAGAAGAGCTGGAAGAGAGAAGGAAGTTGTACCGG
  TGGGGAAACTGGAAGGACGGGTTAATTCTGAATATGGCTGGGGCCAAACTATATGACCTCCCTGTGGATG
  AGCGATTTCTGGAAGACAAGAGAGTTGACTTTGAGGTTTCGCTGGCCAAGGGGCTGGCCGACCTCGCTAT
  CAAAGACTCTCTAAATGTTCTGACTTGCTGGAAGGATCTAGATGACTTCAACCGGATTTTCTGGTGTGGT
  CAGAGCAAGCTGGCTGAGCGCGTGCGGGACTCCTGGAAGGAAGATGCCTTATTTGGGTACCAGTTTCTTA
  ATGGCGCCAACCCCGTGGTGCTGAGGCGCTCTGCTCACCTTCCTGCTCGCCTAGTGTTCCCTCCAGGCAT
  GGAGGAACTGCAGGCCCAGCTGGAGAAGGAGCTGGAGGGAGGCACACTGTTCGAAGCTGACTTCTCCCTG
  CTGGATGGGATCAAGGCCAACGTCATTCTCTGTAGCCAGCAGCACCTGGCTGCCCCTCTAGTCATGCTGA
  AATTGCAGCCTGATGGGAAACTCTTGCCCATGGTCATCCAGCTCCAGCTGCCCCGCACAGGATCCCCACC
  ACCTCCCCTTTTCTTGCCTACGGATCCCCCAATGGCCTGGCTTCTGGCCAAATGCTGGGTGCGCAGCTCT
  GACTTCCAGCTCCATGAGCTGCAGTCTCATCTTCTGAGGGGACACTTGATGGCTGAGGTCATTGTTGTGG
  CCACCATGAGGTGCCTGCCGTCGATACATCCTATCTTCAAGCTTATAATTCCCCACCTGCGATACACCCT
  GGAAATTAACGTCCGGGCCAGGACTGGGCTGGTCTCTGACATGGGAATTTTCGACCAGATAATGAGCACT
  GGTGGGGGAGGCCACGTGCAGCTGCTCAAGCAAGCTGGAGCCTTCCTAACCTACAGCTCCTTCTGTCCCC
  CTGATGACTTGGCCGACCGGGGGCTCCTGGGAGTGAAGTCTTCCTTCTATCCCCAAGATGCGCTGCGGCT
  CTGGGAAATCATCTATCGGTATGTGGAAGGAATCGTGAGTCTCCACTATAAGACAGACGTGGCTGTGAAA
  GACGACCCAGAGCTGCAGACCTGGTGTCGAGAGATCACTGAAATCGGGCTGCAAGGGGCCCAGGACCGAG
  GGTTTCCTGTCTCTTTACAGGCTCGGGACCAGGTTTGCCACTTTGTCACCATGTGTATCTTCACCTGCAC
  CGGCCAACACGCCTCTGTGCACCTGGGCCAGCTGGACTGGTACTCTTGGGTGCCTAATGCACCCTGCACG
  ATGCGGCTGCCCCCGCCAACCACCAAGGATGCAACGCTGGAGACAGTGATGGCGACACTGCCCAACTTCC
  ACCAGGCTTCTCTCCAGATGTCCATCACTTGGCAGCTGGGCAGACGCCAGCCCGTTATGGTGGCTGTGGG
  CCAGCATGAGGAGGAGTATTTTTCGGGCCCTGAGCCTAAGGCTGTGCTGAAGAAGTTCAGGGAGGAGCTG
  GCTGCCCTGGATAAGGAAATTGAGATCCGGAATGCAAAGCTGGACATGCCCTACGAGTACCTGCGGCCCA
  GCGTGGTGGAAAACAGTGTGGCCATC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC206621 representing NM_001140
  Red=Cloning site Green=Tags(s)
  
  MGLYRIRVSTGASLYAGSNNQVQLWLVGQHGEAALGKRLWPARGKETELKVEVPEYLGPLLFVKLRKRHL
  LKDDAWFCNWISVQGPGAGDEVRFPCYRWVEGNGVLSLPEGTGRTVGEDPQGLFQKHREEELEERRKLYR
  WGNWKDGLILNMAGAKLYDLPVDERFLEDKRVDFEVSLAKGLADLAIKDSLNVLTCWKDLDDFNRIFWCG
  QSKLAERVRDSWKEDALFGYQFLNGANPVVLRRSAHLPARLVFPPGMEELQAQLEKELEGGTLFEADFSL
  LDGIKANVILCSQQHLAAPLVMLKLQPDGKLLPMVIQLQLPRTGSPPPPLFLPTDPPMAWLLAKCWVRSS
  DFQLHELQSHLLRGHLMAEVIVVATMRCLPSIHPIFKLIIPHLRYTLEINVRARTGLVSDMGIFDQIMST
  GGGGHVQLLKQAGAFLTYSSFCPPDDLADRGLLGVKSSFYPQDALRLWEIIYRYVEGIVSLHYKTDVAVK
  DDPELQTWCREITEIGLQGAQDRGFPVSLQARDQVCHFVTMCIFTCTGQHASVHLGQLDWYSWVPNAPCT
  MRLPPPTTKDATLETVMATLPNFHQASLQMSITWQLGRRQPVMVAVGQHEEEYFSGPEPKAVLKKFREEL
  AALDKEIEIRNAKLDMPYEYLRPSVVENSVAI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001140
• ORF Size: 1986 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001140.1, NM_001140.2, NM_001140.3, NP_001131.3
• RefSeq Size: 2707 bp
• RefSeq ORF: 1989 bp
• Locus ID: 246
• Cytogenetics: 17p13.2
• Protein Families: Druggable Genome
• Protein Pathways: Arachidonic acid metabolism, Linoleic acid metabolism, Metabolic pathways
• MW: 74.6 kDa
• Gene Summary: 'This gene encodes a member of the lipoxygenase family of proteins. The encoded enzyme acts on various polyunsaturated fatty acid substrates to generate various bioactive lipid mediators such as eicosanoids, hepoxilins, lipoxins, and other molecules. The encoded enzyme and its reaction products have been shown to regulate inflammation and immunity. Multiple pseudogenes of this gene have been identified in the human genome. [provided by RefSeq, Aug 2017]'