CCDC8 (NM_032040) Human Tagged ORF Clone

CAT#: RC206818

CCDC8 (Myc-DDK-tagged)-Human coiled-coil domain containing 8 (CCDC8)

Product Images

Western blot validation of overexpression lysate (Cat# LY410349) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC206818 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CCDC8
• Synonyms: 3M3; p90; PPP1R20
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC206818 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGCAGATCGGGGAGGACGTCGACTATTTGCTCATCCCCCGGGAGGTCAGGCTGGCTGGGGGCGTCT
  GGAGAGTCATCTCTAAGCCCGCCACCAAGGAAGCAGAATTTCGGGAGCGGCTGACCCAGTTCCTGGAAGA
  AGAGGGCCGCACCCTGGAGGACGTGGCCCGCATCATGGAGAAGAGCACCCCGCACCCGCCCCAGCCCCCC
  AAAAAGCCCAAGGAGCCCCGAGTGAGGAGGAGAGTGCAGCAGATGGTGACTCCTCCGCCCCGGCTGGTCG
  TGGGCACGTACGACAGCAGCAACGCCAGCGACAGCGAGTTCAGCGACTTCGAGACCTCCAGAGACAAGAG
  CCGCCAGGGCCCGCGGCGGGGCAAGAAGGTGCGCAAAATGCCCGTCAGCTACCTGGGCAGCAAGTTCCTG
  GGAAGCGACCTGGAGAGTGAGGATGATGAGGAACTGGTCGAGGCCTTCCTCCGGCGACAGGAGAAGCAGC
  CCAGCGCGCCGCCTGCCCGCCGCCGCGTCAACCTGCCAGTGCCCATGTTTGAGGACAACCTGGGGCCTCA
  GCTGTCCAAAGCGGACAGGTGGCGGGAGTATGTCAGCCAGGTGTCCTGGGGGAAGCTGAAGCGGAGGGTG
  AAGGGTTGGGCGCCGAGGGCGGGCCCCGGGGTGGGCGAGGCCCGGCTGGCCTCCACCGCAGTGGAGAGCG
  CAGGGGTATCATCGGCGCCAGAGGGCACCAGCCCGGGGGATCGCTTGGGAAACGCGGGAGATGTTTGTGT
  GCCCCAGGCTTCCCCTAGGCGATGGAGGCCCAAGATCAACTGGGCCTCCTTTCGGCGCCGCAGGAAGGAG
  CAGACAGCACCCACAGGTCAGGGGGCAGACATCGAGGCTGATCAGGGGGGAGAGGCTGCAGATAGTCAAA
  GGGAAGAGGCCATAGCTGACCAGCGGGAAGGGGCTGCAGGTAATCAGAGGGCAGGGGCCCCAGCTGACCA
  GGGGGCAGAGGCTGCAGATAATCAGAGGGAAGAGGCTGCAGATAATCAGAGGGCAGGGGCCCCAGCTGAG
  GAGGGGGCAGAGGCTGCAGATAACCAGAGGGAAGAGGCTGCAGATAATCAGAGGGCAGAGGCCCCAGCTG
  ACCAGAGGTCACAGGGCACAGATAACCACAGGGAAGAGGCTGCAGATAATCAGAGGGCGGAGGCCCCAGC
  TGACCAGGGGTCAGAGGTTACAGATAATCAAAGGGAAGAGGCCGTACATGACCAGAGGGAAAGGGCCCCA
  GCTGTCCAGGGTGCAGATAATCAGAGGGCACAGGCCCGGGCTGGCCAGAGGGCAGAGGCTGCACATAATC
  AGAGGGCAGGGGCCCCAGGTATCCAGGAAGCTGAAGTCTCAGCTGCCCAAGGGACCACAGGAACAGCTCC
  AGGAGCCAGGGCCCGGAAACAGGTCAAGACAGTGAGGTTCCAGACCCCTGGACGCTTTTCGTGGTTTTGC
  AAGCGCCGGAGAGCCTTCTGGCACACTCCCCGGTTGCCAACCCTGCCCAAGAGAGTCCCCAGGGCAGGAG
  AGGCCAGGAACCTCAGGGTGCTGAGGGCCGAGGCCAGAGCAGAAGCTGAGCAGGGAGAGCAAGAAGACCA
  GCTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC206818 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MLQIGEDVDYLLIPREVRLAGGVWRVISKPATKEAEFRERLTQFLEEEGRTLEDVARIMEKSTPHPPQPP
  KKPKEPRVRRRVQQMVTPPPRLVVGTYDSSNASDSEFSDFETSRDKSRQGPRRGKKVRKMPVSYLGSKFL
  GSDLESEDDEELVEAFLRRQEKQPSAPPARRRVNLPVPMFEDNLGPQLSKADRWREYVSQVSWGKLKRRV
  KGWAPRAGPGVGEARLASTAVESAGVSSAPEGTSPGDRLGNAGDVCVPQASPRRWRPKINWASFRRRRKE
  QTAPTGQGADIEADQGGEAADSQREEAIADQREGAAGNQRAGAPADQGAEAADNQREEAADNQRAGAPAE
  EGAEAADNQREEAADNQRAEAPADQRSQGTDNHREEAADNQRAEAPADQGSEVTDNQREEAVHDQRERAP
  AVQGADNQRAQARAGQRAEAAHNQRAGAPGIQEAEVSAAQGTTGTAPGARARKQVKTVRFQTPGRFSWFC
  KRRRAFWHTPRLPTLPKRVPRAGEARNLRVLRAEARAEAEQGEQEDQL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_032040
• ORF Size: 1614 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_032040.1, NM_032040.2, NM_032040.3, NM_032040.4, NP_114429.1
• RefSeq Size: 3334 bp
• RefSeq ORF: 1617 bp
• Locus ID: 83987
• MW: 59.4 kDa
• Gene Summary: This gene encodes a coiled-coil domain-containing protein. The encoded protein functions as a cofactor required for p53-mediated apoptosis following DNA damage, and may also play a role in growth through interactions with the cytoskeletal adaptor protein obscurin-like 1. Mutations in this gene are a cause of 3M syndrome-3 (3M3). [provided by RefSeq, Dec 2011]