CD99L2 (NM_031462) Human Tagged ORF Clone

CAT#: RC207079

CD99L2 (Myc-DDK-tagged)-Human CD99 molecule-like 2 (CD99L2), transcript variant 1


  "NM_031462" in other vectors (4)

Reconstitution Protocol

USD 98.00

USD 390.00

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Size
    • 10 ug

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Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol CD99L2
Synonyms CD99B; MIC2L1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC207079 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGTGGCCTGGCGCTCGGCGTTCCTTGTCTGCCTCGCTTTCTCCTTGGCCACCCTGGTCCAGCGAGGAT
CTGGGGACTTTGATGATTTTAACCTGGAGGATGCAGTGAAAGAAACTTCCTCAGTAAAGCAGCCATGGGA
CCACACCACCACCACCACAACCAATAGGCCAGGAACCACCAGAGCTCCGGCAAAACCTCCAGGTAGTGGA
TTGGACTTGGCTGATGCTTTGGATGATCAAGATGATGGCCGCAGGAAACCGGGTATAGGAGGAAGAGAGA
GATGGAACCATGTAACCACCACGACCAAGAGGCCAGTAACCACCAGAGCTCCAGCAAATACTTTAGGAAA
TGATTTTGACTTGGCTGATGCCCTGGATGATCGAAATGATCGAGATGATGGCCGCAGGAAACCAATTGCT
GGAGGAGGAGGTTTTTCAGACAAGGATCTTGAAGACATAGTAGGGGGTGGAGAATACAAACCTGACAAGG
GTAAAGGTGATGGCCGGTACGGCAGCAATGACGACCCTGGATCTGGCATGGTGGCAGAGCCTGGCACCAT
TGCCGGGGTGGCCAGCGCCCTGGCCATGGCCCTCATCGGTGCCGTCTCCAGCTACATCTCCTACCAGCAG
AAGAAGTTCTGCTTCAGCATTCAGCAGGGTCTCAACGCAGACTACGTGAAGGGAGAGAACCTGGAAGCCG
TGGTATGTGAGGAACCCCAAGTGAAATACTCCACGTTGCACACGCAGTCTGCAGAGCCGCCGCCGCCGCC
CGAACCAGCCCGGATC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC207079 protein sequence
Red=Cloning site Green=Tags(s)

MVAWRSAFLVCLAFSLATLVQRGSGDFDDFNLEDAVKETSSVKQPWDHTTTTTTNRPGTTRAPAKPPGSG
LDLADALDDQDDGRRKPGIGGRERWNHVTTTTKRPVTTRAPANTLGNDFDLADALDDRNDRDDGRRKPIA
GGGGFSDKDLEDIVGGGEYKPDKGKGDGRYGSNDDPGSGMVAEPGTIAGVASALAMALIGAVSSYISYQQ
KKFCFSIQQGLNADYVKGENLEAVVCEEPQVKYSTLHTQSAEPPPPPEPARI

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_031462
ORF Size 786 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_031462.2, NM_031462.3, NP_113650.2
RefSeq Size 3736 bp
RefSeq ORF 789 bp
Locus ID 83692
Protein Families Transmembrane
MW 28 kDa
Gene Summary This gene encodes a cell-surface protein that is similar to CD99. A similar protein in mouse functions as an adhesion molecule during leukocyte extravasation. Alternate splicing results in multiple transcript variants. [provided by RefSeq, May 2010]

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