CSDE1 (NM_007158) Human Tagged ORF Clone

CAT#: RC207101

CSDE1 (Myc-DDK-tagged)-Human cold shock domain containing E1, RNA-binding (CSDE1), transcript variant 2

Product Images

Western blot validation of overexpression lysate (Cat# LY416163) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC207101 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified CSDE1 protein (Cat# TP307101). The protein was produced from HEK293T cells transfected with CSDE1 cDNA clone (Cat# RC207101) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CSDE1
• Synonyms: D1S155E; UNR
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC207101 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCTTTGATCCAAACCTTCTCCACAACAATGGACATAATGGGTACCCTAATGGTACTTCAGCAGCAC
  TGCGTGAAACTGGGGTTATTGAAAAACTGTTAACCTCTTACGGATTTATTCAGTGTTCAGAACGTCAAGC
  TAGACTTTTCTTCCACTGTTCACAGTATAATGGCAACCTGCAAGACTTAAAAGTAGGAGATGATGTTGAA
  TTTGAAGTATCATCGGACCGACGGACTGGGAAACCCATTGCTGTTAAACTGGTGAAGATAAAACAAGAAA
  TCCTCCCTGAAGAACGAATGAATGGACAAGAAGTGTTTTATCTGACTTACACCCCTGAAGATGTCGAAGG
  GAACGTTCAGCTGGAAACTGGAGATAAAATAAACTTTGTAATTGATAACAATAAACATACTGGTGCTGTA
  AGTGCTCGCAACATTATGCTGTTGAAAAAGAAACAAGCCCGCTGTCAGGGAGTAGTTTGTGCCATGAAGG
  AGGCATTTGGCTTTATTGAAAGAGGTGATGTTGTAAAAGAGATATTCTTTCACTATAGTGAATTTAAGGG
  TGACTTAGAAACCTTACAGCCTGGCGATGATGTGGAATTCACAATCAAGGACAGAAATGGTAAAGAAGTT
  GCAACAGATGTCAGACTATTGCCTCAAGGAACAGTCATTTTTGAAGATATCAGCATTGAACATTTTGAAG
  GAACTGTAACCAAAGTTATCCCAAAAGTACCCAGTAAAAACCAGAATGACCCATTGCCAGGACGCATCAA
  AGTTGACTTTGTGATCCCTAAAGAACTTCCCTTTGGAGACAAAGATACGAAATCCAAGGTGACCCTGCTG
  GAAGGTGACCATGTTAGGTTTAATATTTCAACAGACCGACGTGACAAATTAGAGCGAGCAACCAATATAG
  AAGTTCTGTCAAATACATTTCAGTTCACTAATGAAGCCCGAGAAATGGGTGTGATTGCTGCCATGAGAGA
  TGGTTTTGGTTTCATCAAGTGTGTGGATCGTGATGTTCGTATGTTCTTCCACTTCAGTGAAATTCTGGAT
  GGGAACCAGCTCCATATTGCAGATGAAGTAGAGTTTACTGTGGTTCCTGATATGCTCTCTGCTCAAAGAA
  ATCATGCTATTAGGATTAAAAAACTTCCCAAGGGCACGGTTTCATTTCATTCCCATTCAGATCACCGTTT
  TCTGGGCACGGTAGAAAAAGAAGCCACTTTTTCCAATCCTAAAACCACTAGCCCAAATAAAGGCAAAGAG
  AAGGAGGCTGAGGATGGCATTATTGCTTATGATGACTGTGGGGTGAAACTGACTATTGCTTTTCAAGCCA
  AGGATGTGGAAGGATCTACTTCTCCTCAAATAGGAGATAAGGTTGAATTTAGTATTAGTGACAAACAGAG
  GCCTGGACAGCAGGTTGCAACTTGTGTGCGACTTTTAGGTCGTAATTCTAACTCCAAGAGGCTCTTGGGT
  TATGTGGCAACTCTGAAGGATAATTTTGGATTTATTGAAACAGCCAATCATGATAAGGAAATCTTTTTCC
  ATTACAGTGAGTTCTCTGGTGATGTTGATAGCCTGGAACTGGGGGACATGGTCGAGTATAGCTTGTCCAA
  AGGCAAAGGCAACAAAGTCAGTGCAGAAAAAGTGAACAAAACACACTCAGTGAATGGCATTACTGAGGAA
  GCTGATCCCACCATTTACTCTGGCAAAGTAATTCGCCCCCTGAGGAGTGTTGATCCAACACAGACTGAGT
  ACCAAGGAATGATTGAGATTGTGGAGGAGGGCGATATGAAAGGTGAGGTCTATCCATTTGGCATCGTTGG
  GATGGCCAACAAAGGGGATTGCCTGCAGAAAGGGGAGAGCGTCAAGTTCCAATTGTGTGTCCTGGGCCAA
  AATGCACAAACTATGGCTTACAACATCACACCCCTGCGCAGGGCCACAGTGGAATGTGTGAAAGATCAGT
  TTGGCTTCATTAACTATGAAGTAGGAGATAGCAAGAAGCTCTTTTTCCATGTGAAAGAAGTTCAGGATGG
  CATTGAGCTACAGGCAGGAGATGAGGTGGAGTTCTCAGTGATTCTTAATCAGCGCACTGGCAAGTGCAGC
  GCCTGTAATGTTTGGCGAGTCTGTGAGGGCCCCAAGGCTGTTGCAGCTCCTCGACCTGATCGGTTGGTCA
  ATCGCTTGAAGAATATCACTCTGGATGATGCCAGTGCTCCTCGCCTAATGGTTCTTCGTCAGCCAAGGGG
  ACCAGATAACTCAATGGGGTTTGGTGCAGAAAGAAAGATCCGTCAAGCTGGTGTCATTGAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC207101 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSFDPNLLHNNGHNGYPNGTSAALRETGVIEKLLTSYGFIQCSERQARLFFHCSQYNGNLQDLKVGDDVE
  FEVSSDRRTGKPIAVKLVKIKQEILPEERMNGQEVFYLTYTPEDVEGNVQLETGDKINFVIDNNKHTGAV
  SARNIMLLKKKQARCQGVVCAMKEAFGFIERGDVVKEIFFHYSEFKGDLETLQPGDDVEFTIKDRNGKEV
  ATDVRLLPQGTVIFEDISIEHFEGTVTKVIPKVPSKNQNDPLPGRIKVDFVIPKELPFGDKDTKSKVTLL
  EGDHVRFNISTDRRDKLERATNIEVLSNTFQFTNEAREMGVIAAMRDGFGFIKCVDRDVRMFFHFSEILD
  GNQLHIADEVEFTVVPDMLSAQRNHAIRIKKLPKGTVSFHSHSDHRFLGTVEKEATFSNPKTTSPNKGKE
  KEAEDGIIAYDDCGVKLTIAFQAKDVEGSTSPQIGDKVEFSISDKQRPGQQVATCVRLLGRNSNSKRLLG
  YVATLKDNFGFIETANHDKEIFFHYSEFSGDVDSLELGDMVEYSLSKGKGNKVSAEKVNKTHSVNGITEE
  ADPTIYSGKVIRPLRSVDPTQTEYQGMIEIVEEGDMKGEVYPFGIVGMANKGDCLQKGESVKFQLCVLGQ
  NAQTMAYNITPLRRATVECVKDQFGFINYEVGDSKKLFFHVKEVQDGIELQAGDEVEFSVILNQRTGKCS
  ACNVWRVCEGPKAVAAPRPDRLVNRLKNITLDDASAPRLMVLRQPRGPDNSMGFGAERKIRQAGVID
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_007158
• ORF Size: 2301 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_007158.4, NM_007158.5, NP_009089.4
• RefSeq Size: 4090 bp
• RefSeq ORF: 2304 bp
• Locus ID: 7812
• Domains: CSD
• Protein Families: Transcription Factors
• MW: 85.7 kDa