Choline kinase alpha (CHKA) (NM_212469) Human Tagged ORF Clone

CAT#: RC207209

CHKA (Myc-DDK-tagged)-Human choline kinase alpha (CHKA), transcript variant 2

Product Images

Western blot validation of overexpression lysate (Cat# LY403943) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC207209 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified CHKA protein (Cat# TP307209). The protein was produced from HEK293T cells transfected with CHKA cDNA clone (Cat# RC207209) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CHKA
• Synonyms: CHK; CK; CKI; EK
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC207209 representing NM_212469
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAAACCAAATTCTGCACCGGGGGCGAGGCGGAGCCCTCGCCGCTCGGGCTGCTGCTGAGCTGCGGTA
  GCGGCAGCGCGGCCCCGGCGCCCGGCGTGGGGCAGCAGCGCGACGCCGCCAGCGACCTCGAGTCCAAGCA
  GCTGGGCGGCCAACAGCCGCCGCTCGCGCTGCCCCCTCCGCCGCCGCTGCCGCTGCCGCTGCCGCTGCCC
  CAGCCCCCGCCGCCGCAGCCGCCCGCAGACGAGCAGCCGGAGCCCCGGACGCGGCGCAGGGCCTATCTGT
  GGTGCAAGGAGTTCCTGCCCGGCGCCTGGCGGGGCCTCCGCGAGGACGAGTTCCACATCAGTGTCATCAG
  AGGCGGCCTTAGCAACATGCTGTTCCAGTGCTCCCTACCTGACACCACAGCCACCCTTGGTGATGAGCCT
  CGGAAAGTGCTCCTGCGGCTGTATGGAGCGATTTTGCAGGTGGGGGCTGAGGCCATGGTTCTGGAGAGCG
  TTATGTTTGCCATTCTCGCAGAGAGGTCACTTGGGCCAAAACTCTATGGCATCTTTCCCCAAGGCCGACT
  GGAGCAGTTCATCCCGAGCCGGCGATTAGATACTGAAGAATTAGGTTTGCCAGATATTTCTGCAGAAATC
  GCCGAGAAAATGGCTACATTTCATGGTATGAAAATGCCATTCAATAAGGAACCAAAATGGCTTTTTGGCA
  CAATGGAAAAGTATCTAAAGGAAGTGCTGAGAATTAAATTTACTGAGGAATCCAGAATTAAAAAGCTCCA
  CAAATTGCTCAGTTACAATCTGCCCTTGGAACTGGAAAACCTGAGATCATTGCTTGAATCTACTCCATCT
  CCAGTTGTATTTTGTCATAATGACTGTCAAGAAGGTAATATCTTGTTGCTGGAAGGCCGAGAGAATTCTG
  AAAAACAGAAACTGATGCTCATTGATTTCGAATACAGCAGTTACAATTACAGGGGATTCGACATTGGAAA
  TCACTTCTGTGAGTGGATGTATGATTATAGCTATGAAAAATACCCTTTTTTCAGAGCAAACATCCGGAAG
  TATCCCACCAAGAAACAACAGCTCCATTTTATTTCCAGTTACTTGCCTGCATTCCAAAATGACTTTGAAA
  ACCTCAGTACTGAAGAAAAATCCATTATAAAAGAAGAAATGTTGCTTGAAGTTAATAGGTTTGCCCTTGC
  ATCTCATTTCCTCTGGGGACAGTGGTCCATTGTACAAGCCAAGATTTCATCTATTGAATTTGGGTACATG
  GACTACGCCCAAGCAAGGTTTGATGCCTATTTCCACCAGAAGAGGAAGCTTGGGGTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC207209 representing NM_212469
  Red=Cloning site Green=Tags(s)
  
  MKTKFCTGGEAEPSPLGLLLSCGSGSAAPAPGVGQQRDAASDLESKQLGGQQPPLALPPPPPLPLPLPLP
  QPPPPQPPADEQPEPRTRRRAYLWCKEFLPGAWRGLREDEFHISVIRGGLSNMLFQCSLPDTTATLGDEP
  RKVLLRLYGAILQVGAEAMVLESVMFAILAERSLGPKLYGIFPQGRLEQFIPSRRLDTEELGLPDISAEI
  AEKMATFHGMKMPFNKEPKWLFGTMEKYLKEVLRIKFTEESRIKKLHKLLSYNLPLELENLRSLLESTPS
  PVVFCHNDCQEGNILLLEGRENSEKQKLMLIDFEYSSYNYRGFDIGNHFCEWMYDYSYEKYPFFRANIRK
  YPTKKQQLHFISSYLPAFQNDFENLSTEEKSIIKEEMLLEVNRFALASHFLWGQWSIVQAKISSIEFGYM
  DYAQARFDAYFHQKRKLGV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_212469
• ORF Size: 1317 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_212469.1, NP_997634.1
• RefSeq Size: 2679 bp
• RefSeq ORF: 1320 bp
• Locus ID: 1119
• Cytogenetics: 11q13.2
• Protein Families: Druggable Genome
• Protein Pathways: Glycerophospholipid metabolism, Metabolic pathways
• MW: 50 kDa
• Gene Summary: 'The major pathway for the biosynthesis of phosphatidylcholine occurs via the CDP-choline pathway. The protein encoded by this gene is the initial enzyme in the sequence and may play a regulatory role. The encoded protein also catalyzes the phosphorylation of ethanolamine. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]'