DHDH (NM_014475) Human Tagged ORF Clone

CAT#: RC207537

DHDH (Myc-DDK-tagged)-Human dihydrodiol dehydrogenase (dimeric) (DHDH)

Product Images

Western blot validation of overexpression lysate (Cat# LY415257) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC207537 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified DHDH protein (Cat# TP307537). The protein was produced from HEK293T cells transfected with DHDH cDNA clone (Cat# RC207537) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: DHDH
• Synonyms: 2DD; HUM2DD
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC207537 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGCTGCGCTGGGGCATCGTGTCTGTCGGCCTCATCTCCAGCGACTTCACAGCCGTGCTGCAGACGC
  TGCCTCGCTCTGAGCACCAGGTGGTGGCGGTGGCGGCCCGCGATCTGAGCCGTGCGAAGGAGTTTGCACA
  GAAACACGACATCCCCAAGGCCTACGGCTCCTATGAGGAGCTGGCCAAGGACCCGAGCGTGGAGGTGGCC
  TACATTGGCACCCAGCACCCCCAGCACAAGGCGGCGGTGATGCTGTGCTTGGCGGCGGGCAAGGCCGTTC
  TGTGCGAGAAGCCCACGGGCGTGAACGCGGCGGAAGTTCGCGAGATGGTCGCGGAGGCCCGATCCCGAGC
  CCTCTTCCTTATGGAGGCCATCTGGACCCGCTTCTTTCCTGCCTCCGAGGCTCTGAGGTCTGTTTTGGCC
  CAGGGAACTCTAGGAGACCTCCGGGTGGCTCGGGCAGAATTTGGGAAGAATCTCATCCACGTTCCCCGGG
  CCGTAGACCGGGCCCAGGCTGGGGGGGCCCTGCTGGACATCGGCATCTACTGTGTCCAGTTCACCTCCAT
  GGTCTTTGGAGGGCAGAAGCCAGAGAAGATTTCTGTCGTGGGAAGGCGTCATGAAACAGGTGTGGATGAC
  ACTGTCACGGTGCTCCTGCAGTACCCAGGGGAGGTCCATGGCAGCTTCACCTGCAGCATCACCGTGCAGC
  TCTCCAACACGGCCTCCGTGAGCGGCACCAAGGGCATGGTACAGCTCCTCAACCCCTGCTGGTGCCCGAC
  CGAGCTGGTGGTGAAGGGGGAGCATAAGGAGTTCCCGCTGCCCCCAGTCCCAAAGGACTGCAATTTTGAC
  AACGGGGCAGGCATGAGTTATGAGGCCAAGCACGTCTGGGAGTGCCTACGCAAGGGTATGAAGGAAAGTC
  CTGTGATTCCCCTGTCGGAAAGTGAGCTCCTGGCTGACATCCTTGAAGAGGTGAGGAAGGCCATTGGAGT
  CACCTTCCCCCAAGACAAACGC
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC207537 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MALRWGIVSVGLISSDFTAVLQTLPRSEHQVVAVAARDLSRAKEFAQKHDIPKAYGSYEELAKDPSVEVA
  YIGTQHPQHKAAVMLCLAAGKAVLCEKPTGVNAAEVREMVAEARSRALFLMEAIWTRFFPASEALRSVLA
  QGTLGDLRVARAEFGKNLIHVPRAVDRAQAGGALLDIGIYCVQFTSMVFGGQKPEKISVVGRRHETGVDD
  TVTVLLQYPGEVHGSFTCSITVQLSNTASVSGTKGMVQLLNPCWCPTELVVKGEHKEFPLPPVPKDCNFD
  NGAGMSYEAKHVWECLRKGMKESPVIPLSESELLADILEEVRKAIGVTFPQDKR
  
  SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_014475
• ORF Size: 1002 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014475.1, NM_014475.2, NM_014475.3, NP_055290.1
• RefSeq Size: 1098 bp
• RefSeq ORF: 1005 bp
• Locus ID: 27294
• Protein Pathways: Metabolism of xenobiotics by cytochrome P450
• MW: 36.4 kDa
• Gene Summary: This gene encodes an enzyme that belongs to the family of dihydrodiol dehydrogenases, which exist in multiple forms in mammalian tissues and are involved in the metabolism of xenobiotics and sugars. These enzymes catalyze the NADP1-linked oxidation of transdihydrodiols of aromatic hydrocarbons to corresponding catechols. This enzyme is a dimeric dihydrodiol dehydrogenase, and it differs from monomeric dihydrodiol dehydrogenases in its high substrate specificity for trans-dihydrodiols of aromatic hydrocarbons in the oxidative direction. [provided by RefSeq, Jul 2008]