Angiomotin like 1 (AMOTL1) (NM_130847) Human Tagged ORF Clone

CAT#: RC207693

AMOTL1 (Myc-DDK-tagged)-Human angiomotin like 1 (AMOTL1)

Product Images

Western blot validation of overexpression lysate (Cat# LY408899) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC207693 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: AMOTL1
• Synonyms: JEAP
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC207693 representing NM_130847
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTGGAGGGCAAAGTTGCGCCGGGGAACTTGTGAGCCTGCGGTGAAAGGGTCCCCTTCTGCTTGTTATA
  GCCCCAGTAGTCCTGTCCAGGTTCTAGAAGACTCCACCTACTTTTCCCCAGACTTTCAGCTCTATTCTGG
  GAGGCATGAAACATCTGCTTTGACGGTGGAGGCAACCAGTAGCATCAGGGAAAAAGTTGTTGAAGATCCT
  CTTTGTAACTTCCACTCCCCAAACTTCCTGAGGATCTCAGAGGTGGAAATGAGAGGTTCCGAGGATGCGG
  CAGCTGGAACAGTATTGCAGCGGCTGATCCAGGAACAACTGCGGTATGGCACCCCAACCGAGAACATGAA
  CTTGCTGGCCATTCAGCACCAGGCCACAGGGAGTGCAGGACCAGCCCATCCTACAAACAACTTTTCTTCC
  ACGGAAAACCTCACTCAAGAAGACCCACAAATGGTCTACCAGTCAGCACGCCAAGAACCGCAGGGTCAAG
  AACACCAGGTGGACAATACGGTGATGGAGAAACAGGTCCGGTCCACGCAGCCTCAGCAGAACAACGAGGA
  ACTGCCCACTTACGAGGAGGCCAAAGCACAGTCGCAGTTCTTCAGGGGGCAGCAGCAGCAGCAACAGCAG
  CAGGGGGCGGTGGGCCATGGTTACTACATGGCAGGGGGCACCAGTCAGAAGTCCCGAACTGAGGGGAGGC
  CCACTGTGAACCGTGCCAACAGTGGACAGGCGCATAAGGACGAGGCGCTGAAGGAACTGAAGCAGGGCCA
  CGTCCGCTCGCTCAGCGAGAGAATCATGCAGCTGTCCCTGGAGAGGAATGGGGCCAAGCAACACCTTCCC
  GGCTCGGGGAATGGAAAGGGCTTCAAAGTAGGAGGGGGGCCCTCCCCTGCCCAGCCTGCAGGTAAAGTGC
  TGGACCCTCGGGGTCCTCCACCTGAGTACCCCTTCAAGACCAAGCAAATGATGTCCCCAGTCAGCAAGAC
  CCAGGAGCACGGACTTTTTTATGGTGACCAGCACCCCGGGATGCTCCACGAGATGGTCAAGCCCTACCCT
  GCTCCTCAGCCTGTGAGAACAGATGTGGCCGTCCTGCGGTACCAGCCACCCCCTGAGTATGGGGTAACGA
  GCCGCCCATGCCAACTTCCGTTCCCATCAACCATGCAGCAGCACAGCCCCATGTCCTCCCAGACCTCTTC
  CGCCAGCGGGCCACTGCACTCTGTCTCCCTGCCGCTTCCACTCCCGATGGCCCTGGGTGCTCCACAGCCC
  CCGCCTGCCGCCTCCCCCAGCCAGCAGCTTGGTCCAGATGCCTTTGCGATTGTGGAGCGAGCCCAGCAAA
  TGGTGGAGATATTAACAGAGGAGAACCGGGTGCTTCACCAGGAACTTCAGGGTTACTACGACAATGCCGA
  CAAGCTCCACAAGTTTGAAAAAGAACTTCAGAGAATTTCGGAAGCCTATGAAAGTCTGGTCAAGTCTACC
  ACCAAGCGAGAATCGCTGGACAAGGCCATGAGAAACAAATTGGAAGGCGAGATTAGAAGACTTCATGATT
  TCAACAGAGACCTCCGAGATCGACTAGAGACTGCTAACAGGCAACTATCCAGCAGGGAATACGAAGGGCA
  TGAAGACAAAGCTGCAGAGGGGCATTATGCTTCCCAGAACAAAGAATTCTTGAAGGAAAAGGAGAAATTA
  GAAATGGAGTTAGCAGCAGTGCGGACTGCAAGTGAGGACCATCGGAGACACATCGAGATCCTGGACCAGG
  CTTTGAGCAACGCCCAGGCCAGGGTCATCAAGCTGGAAGAGGAGTTACGAGAGAAGCAAGCATATGTTGA
  GAAAGTTGAGAAGCTGCAGCAGGCCCTGACCCAGCTGCAGTCTGCATGTGAGAAGCGAGAACAGATGGAG
  CGGAGACTGCGGACTTGGCTGGAGAGAGAGCTGGATGCACTGAGAACCCAGCAGAAACATGGAAATGGCC
  AGCCAGCCAACATGCCGGAATACAATGCCCCAGCCCTCCTGGAACTTGTGCGGGAGAAGGAGGAGCGGAT
  CCTGGCCCTGGAGGCCGACATGACAAAGTGGGAGCAGAAGTACCTGGAGGAGAGCACCATCCGACACTTT
  GCCATGAATGCCGCAGCCACTGCAGCAGCTGAGAGGGACACCACGATCATCAACCACTCACGGAATGGCA
  GCTACGGAGAGAGCTCGCTGGAGGCCCACATCTGGCAAGAGGAGGAGGAGGTGGTGCAGGCCAACAGAAG
  GTGTCAGGACATGGAATACACTATTAAAAATCTCCATGCCAAAATCATAGAGAAAGATGCTATGATTAAG
  GTCCTGCAGCAGCGATCTCGTAAAGATGCCGGGAAGACAGACTCCTCCAGCCTACGTCCTGCCCGCTCCG
  TTCCATCCATAGCAGCAGCTACTGGGACACACTCTCGCCAGACCTCTCTTACCAGCAGCCAGCTGGCTGA
  GGAAAAGAAGGAAGAGAAGACCTGGAAGGGGAGCATAGGATTGCTGCTGGGGAAGGAGCACCATGAGCAT
  GCCTCTGCCCCACTGCTGCCACCCCCACCCACCTCAGCACTGTCCTCCATAGCCTCCACTACGGCAGCCA
  GCAGTGCCCACGCCAAGACAGGCAGCAAGGACAGCAGCACACAGACTGACAAGAGTGCCGAGCTCTTCTG
  GCCCAGCATGGCCTCCCTTCCCAGCCGCGGCCGGCTGAGCACGACCCCTGCTCACAGCCCCGTCCTGAAA
  CACCCAGCGGCCAAAGGGACCGCAGAGAAACTGGAGAACTCTCCTGGCCATGGGAAGTCGCCTGACCACA
  GAGGCCGGGTCAGCAGCTTGCTGCACAAGCCCGAGTTCCCTGATGGAGAGATGATGGAAGTCCTCATC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC207693 representing NM_130847
  Red=Cloning site Green=Tags(s)
  
  MWRAKLRRGTCEPAVKGSPSACYSPSSPVQVLEDSTYFSPDFQLYSGRHETSALTVEATSSIREKVVEDP
  LCNFHSPNFLRISEVEMRGSEDAAAGTVLQRLIQEQLRYGTPTENMNLLAIQHQATGSAGPAHPTNNFSS
  TENLTQEDPQMVYQSARQEPQGQEHQVDNTVMEKQVRSTQPQQNNEELPTYEEAKAQSQFFRGQQQQQQQ
  QGAVGHGYYMAGGTSQKSRTEGRPTVNRANSGQAHKDEALKELKQGHVRSLSERIMQLSLERNGAKQHLP
  GSGNGKGFKVGGGPSPAQPAGKVLDPRGPPPEYPFKTKQMMSPVSKTQEHGLFYGDQHPGMLHEMVKPYP
  APQPVRTDVAVLRYQPPPEYGVTSRPCQLPFPSTMQQHSPMSSQTSSASGPLHSVSLPLPLPMALGAPQP
  PPAASPSQQLGPDAFAIVERAQQMVEILTEENRVLHQELQGYYDNADKLHKFEKELQRISEAYESLVKST
  TKRESLDKAMRNKLEGEIRRLHDFNRDLRDRLETANRQLSSREYEGHEDKAAEGHYASQNKEFLKEKEKL
  EMELAAVRTASEDHRRHIEILDQALSNAQARVIKLEEELREKQAYVEKVEKLQQALTQLQSACEKREQME
  RRLRTWLERELDALRTQQKHGNGQPANMPEYNAPALLELVREKEERILALEADMTKWEQKYLEESTIRHF
  AMNAAATAAAERDTTIINHSRNGSYGESSLEAHIWQEEEEVVQANRRCQDMEYTIKNLHAKIIEKDAMIK
  VLQQRSRKDAGKTDSSSLRPARSVPSIAAATGTHSRQTSLTSSQLAEEKKEEKTWKGSIGLLLGKEHHEH
  ASAPLLPPPPTSALSSIASTTAASSAHAKTGSKDSSTQTDKSAELFWPSMASLPSRGRLSTTPAHSPVLK
  HPAAKGTAEKLENSPGHGKSPDHRGRVSSLLHKPEFPDGEMMEVLI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_130847
• ORF Size: 2868 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_130847.1, NM_130847.2, NP_570899.1
• RefSeq Size: 8999
• RefSeq ORF: 2871
• Locus ID: 154810
• Protein Pathways: Tight junction
• MW: 106.4 kDa
• Gene Summary: The protein encoded by this gene is a peripheral membrane protein that is a component of tight junctions or TJs. TJs form an apical junctional structure and act to control paracellular permeability and maintain cell polarity. This protein is related to angiomotin, an angiostatin binding protein that regulates endothelial cell migration and capillary formation. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2014]