CD163 (NM_004244) Human Tagged ORF Clone

CAT#: RC208557

CD163 (Myc-DDK-tagged)-Human CD163 molecule (CD163), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY401362) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC208557 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified CD163 protein (Cat# TP308557). The protein was produced from HEK293T cells transfected with CD163 cDNA clone (Cat# RC208557) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CD163
• Synonyms: M130; MM130; SCARI1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC208557 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCAAACTCAGAATGGTGCTACTTGAAGACTCTGGATCTGCTGACTTCAGAAGACATTTTGTCAACT
  TGAGTCCCTTCACCATTACTGTGGTCTTACTTCTCAGTGCCTGTTTTGTCACCAGTTCTCTTGGAGGAAC
  AGACAAGGAGCTGAGGCTAGTGGATGGTGAAAACAAGTGTAGCGGGAGAGTGGAAGTGAAAGTCCAGGAG
  GAGTGGGGAACGGTGTGTAATAATGGCTGGAGCATGGAAGCGGTCTCTGTGATTTGTAACCAGCTGGGAT
  GTCCAACTGCTATCAAAGCCCCTGGATGGGCTAATTCCAGTGCAGGTTCTGGACGCATTTGGATGGATCA
  TGTTTCTTGTCGTGGGAATGAGTCAGCTCTTTGGGATTGCAAACATGATGGATGGGGAAAGCATAGTAAC
  TGTACTCACCAACAAGATGCTGGAGTGACCTGCTCAGATGGATCCAATTTGGAAATGAGGCTGACGCGTG
  GAGGGAATATGTGTTCTGGAAGAATAGAGATCAAATTCCAAGGACGGTGGGGAACAGTGTGTGATGATAA
  CTTCAACATAGATCATGCATCTGTCATTTGTAGACAACTTGAATGTGGAAGTGCTGTCAGTTTCTCTGGT
  TCATCTAATTTTGGAGAAGGCTCTGGACCAATCTGGTTTGATGATCTTATATGCAACGGAAATGAGTCAG
  CTCTCTGGAACTGCAAACATCAAGGATGGGGAAAGCATAACTGTGATCATGCTGAGGATGCTGGAGTGAT
  TTGCTCAAAGGGAGCAGATCTGAGCCTGAGACTGGTAGATGGAGTCACTGAATGTTCAGGAAGATTAGAA
  GTGAGATTCCAAGGAGAATGGGGGACAATATGTGATGACGGCTGGGACAGTTACGATGCTGCTGTGGCAT
  GCAAGCAACTGGGATGTCCAACTGCCGTCACAGCCATTGGTCGAGTTAACGCCAGTAAGGGATTTGGACA
  CATCTGGCTTGACAGCGTTTCTTGCCAGGGACATGAACCTGCTGTCTGGCAATGTAAACACCATGAATGG
  GGAAAGCATTATTGCAATCACAATGAAGATGCTGGCGTGACATGTTCTGATGGATCAGATCTGGAGCTAA
  GACTTAGAGGTGGAGGCAGCCGCTGTGCTGGGACAGTTGAGGTGGAGATTCAGAGACTGTTAGGGAAGGT
  GTGTGACAGAGGCTGGGGACTGAAAGAAGCTGATGTGGTTTGCAGGCAGCTGGGATGTGGATCTGCACTC
  AAAACATCTTATCAAGTGTACTCCAAAATCCAGGCAACAAACACATGGCTGTTTCTAAGTAGCTGTAACG
  GAAATGAAACTTCTCTTTGGGACTGCAAGAACTGGCAATGGGGTGGACTTACCTGTGATCACTATGAAGA
  AGCCAAAATTACCTGCTCAGCCCACAGGGAACCCAGACTGGTTGGAGGGGACATTCCCTGTTCTGGACGT
  GTTGAAGTGAAGCATGGTGACACGTGGGGCTCCATCTGTGATTCGGACTTCTCTCTGGAAGCTGCCAGCG
  TTCTATGCAGGGAATTACAGTGTGGCACAGTTGTCTCTATCCTGGGGGGAGCTCACTTTGGAGAGGGAAA
  TGGACAGATCTGGGCTGAAGAATTCCAGTGTGAGGGACATGAGTCCCATCTTTCACTCTGCCCAGTAGCA
  CCCCGCCCAGAAGGAACTTGTAGCCACAGCAGGGATGTTGGAGTAGTCTGCTCAAGATACACAGAAATTC
  GCTTGGTGAATGGCAAGACCCCGTGTGAGGGCAGAGTGGAGCTCAAAACGCTTGGTGCCTGGGGATCCCT
  CTGTAACTCTCACTGGGACATAGAAGATGCCCATGTTCTTTGCCAGCAGCTTAAATGTGGAGTTGCCCTT
  TCTACCCCAGGAGGAGCACGTTTTGGAAAAGGAAATGGTCAGATCTGGAGGCATATGTTTCACTGCACTG
  GGACTGAGCAGCACATGGGAGATTGTCCTGTAACTGCTCTAGGTGCTTCATTATGTCCTTCAGAGCAAGT
  GGCCTCTGTAATCTGCTCAGGAAACCAGTCCCAAACACTGTCCTCGTGCAATTCATCGTCTTTGGGCCCA
  ACAAGGCCTACCATTCCAGAAGAAAGTGCTGTGGCCTGCATAGAGAGTGGTCAACTTCGCCTGGTAAATG
  GAGGAGGTCGCTGTGCTGGGAGAGTAGAGATCTATCATGAGGGCTCCTGGGGCACCATCTGTGATGACAG
  CTGGGACCTGAGTGATGCCCACGTGGTTTGCAGACAGCTGGGCTGTGGAGAGGCCATTAATGCCACTGGT
  TCTGCTCATTTTGGGGAAGGAACAGGGCCCATCTGGCTGGATGAGATGAAATGCAATGGAAAAGAATCCC
  GCATTTGGCAGTGCCATTCACACGGCTGGGGGCAGCAAAATTGCAGGCACAAGGAGGATGCGGGAGTTAT
  CTGCTCAGAATTCATGTCTCTGAGACTGACCAGTGAAGCCAGCAGAGAGGCCTGTGCAGGGCGTCTGGAA
  GTTTTTTACAATGGAGCTTGGGGCACTGTTGGCAAGAGTAGCATGTCTGAAACCACTGTGGGTGTGGTGT
  GCAGGCAGCTGGGCTGTGCAGACAAAGGGAAAATCAACCCTGCATCTTTAGACAAGGCCATGTCCATTCC
  CATGTGGGTGGACAATGTTCAGTGTCCAAAAGGACCTGACACGCTGTGGCAGTGCCCATCATCTCCATGG
  GAGAAGAGACTGGCCAGCCCCTCGGAGGAGACCTGGATCACATGTGACAACAAGATAAGACTTCAGGAAG
  GACCCACTTCCTGTTCTGGACGTGTGGAGATCTGGCATGGAGGTTCCTGGGGGACAGTGTGTGATGACTC
  TTGGGACTTGGACGATGCTCAGGTGGTGTGTCAACAACTTGGCTGTGGTCCAGCTTTGAAAGCATTCAAA
  GAAGCAGAGTTTGGTCAGGGGACTGGACCGATATGGCTCAATGAAGTGAAGTGCAAAGGGAATGAGTCTT
  CCTTGTGGGATTGTCCTGCCAGACGCTGGGGCCATAGTGAGTGTGGGCACAAGGAAGACGCTGCAGTGAA
  TTGCACAGATATTTCAGTGCAGAAAACCCCACAAAAAGCCACAACAGGTCGCTCATCCCGTCAGTCATCC
  TTTATTGCAGTCGGGATCCTTGGGGTTGTTCTGTTGGCCATTTTCGTCGCATTATTCTTCTTGACTAAAA
  AGCGAAGACAGAGACAGCGGCTTGCAGTTTCCTCAAGAGGAGAGAACTTAGTCCACCAAATTCAATACCG
  GGAGATGAATTCTTGCCTGAATGCAGATGATCTGGACCTAATGAATTCCTCAGAAAATTCCCATGAGTCA
  GCTGATTTCAGTGCTGCTGAACTAATTTCTGTGTCTAAATTTCTTCCTATTTCTGGAATGGAAAAGGAGG
  CCATTCTGAGCCACACTGAAAAGGAAAATGGGAATTTA
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC208557 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSKLRMVLLEDSGSADFRRHFVNLSPFTITVVLLLSACFVTSSLGGTDKELRLVDGENKCSGRVEVKVQE
  EWGTVCNNGWSMEAVSVICNQLGCPTAIKAPGWANSSAGSGRIWMDHVSCRGNESALWDCKHDGWGKHSN
  CTHQQDAGVTCSDGSNLEMRLTRGGNMCSGRIEIKFQGRWGTVCDDNFNIDHASVICRQLECGSAVSFSG
  SSNFGEGSGPIWFDDLICNGNESALWNCKHQGWGKHNCDHAEDAGVICSKGADLSLRLVDGVTECSGRLE
  VRFQGEWGTICDDGWDSYDAAVACKQLGCPTAVTAIGRVNASKGFGHIWLDSVSCQGHEPAVWQCKHHEW
  GKHYCNHNEDAGVTCSDGSDLELRLRGGGSRCAGTVEVEIQRLLGKVCDRGWGLKEADVVCRQLGCGSAL
  KTSYQVYSKIQATNTWLFLSSCNGNETSLWDCKNWQWGGLTCDHYEEAKITCSAHREPRLVGGDIPCSGR
  VEVKHGDTWGSICDSDFSLEAASVLCRELQCGTVVSILGGAHFGEGNGQIWAEEFQCEGHESHLSLCPVA
  PRPEGTCSHSRDVGVVCSRYTEIRLVNGKTPCEGRVELKTLGAWGSLCNSHWDIEDAHVLCQQLKCGVAL
  STPGGARFGKGNGQIWRHMFHCTGTEQHMGDCPVTALGASLCPSEQVASVICSGNQSQTLSSCNSSSLGP
  TRPTIPEESAVACIESGQLRLVNGGGRCAGRVEIYHEGSWGTICDDSWDLSDAHVVCRQLGCGEAINATG
  SAHFGEGTGPIWLDEMKCNGKESRIWQCHSHGWGQQNCRHKEDAGVICSEFMSLRLTSEASREACAGRLE
  VFYNGAWGTVGKSSMSETTVGVVCRQLGCADKGKINPASLDKAMSIPMWVDNVQCPKGPDTLWQCPSSPW
  EKRLASPSEETWITCDNKIRLQEGPTSCSGRVEIWHGGSWGTVCDDSWDLDDAQVVCQQLGCGPALKAFK
  EAEFGQGTGPIWLNEVKCKGNESSLWDCPARRWGHSECGHKEDAAVNCTDISVQKTPQKATTGRSSRQSS
  FIAVGILGVVLLAIFVALFFLTKKRRQRQRLAVSSRGENLVHQIQYREMNSCLNADDLDLMNSSENSHES
  ADFSAAELISVSKFLPISGMEKEAILSHTEKENGNL
  
  SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_004244
• ORF Size: 3471 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004244.3, NM_004244.4, NM_004244.5, NP_004235.3
• RefSeq Size: 4190 bp
• RefSeq ORF: 3471 bp
• Locus ID: 9332
• Domains: SR
• Protein Families: Druggable Genome, Secreted Protein, Transmembrane
• MW: 125.4 kDa
• Gene Summary: The protein encoded by this gene is a member of the scavenger receptor cysteine-rich (SRCR) superfamily, and is exclusively expressed in monocytes and macrophages. It functions as an acute phase-regulated receptor involved in the clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages, and may thereby protect tissues from free hemoglobin-mediated oxidative damage. This protein may also function as an innate immune sensor for bacteria and inducer of local inflammation. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Aug 2011]