MRRF (NM_199177) Human Tagged ORF Clone
CAT#: RC208798
MRRF (Myc-DDK-tagged)-Human mitochondrial ribosome recycling factor (MRRF), nuclear gene encoding mitochondrial protein, transcript variant 2
"NM_199177" in other vectors (4)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | MRRF |
Synonyms | MRFF; MTRRF; RRF |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC208798 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCTTGGGATTAAAGTGCTTCCGCATGGTCCACCCTACCTTTCGCAATTATCTTGCAGCCTCTATCA GACCCGTTTCAGAAGTTACACTGAAGACAGTGCATGAAAGACAACATGGCCATAGGCAATACATGGCCTA TTCAGCTGTACCAGTCCGCCATTTTGCTACCAAGAAAGCCAAAGCCAAAGGGAAAGGACAGTCCCAAACC AGAGTGAATATTAATGCTGCCTTGGTTGAGGATATAATCAACTTGGAAGAGGTGAATGAAGAAATGAAGT CTGTGATAGAAGCTCTCAAGGATAATTTCAATAAGACTCTCAATATAAGGACCTCACCAGGATCCCTTGA CAAGATTGCTGTGGTAACTGCTGACGGGAAGCTTGCTTTAAACCAGATTAGCCAGATCTCCATGAAGTCG CCACAGCTGATTTTGGTGAATATGGCCAGCTTCCCAGAGTGTACAGCTGCAGCTATCAAGGCTATAAGAG AAAGTGGAATGAATCTGAACCCAGAAGTGGAAGGGACGCTAATTCGGGTACCCATTCCCCAATCAGCCAA ATGGCCGATGACACAGTGGCAGAACTGGACAGGCATCTGGCAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC208798 protein sequence
Red=Cloning site Green=Tags(s) MALGLKCFRMVHPTFRNYLAASIRPVSEVTLKTVHERQHGHRQYMAYSAVPVRHFATKKAKAKGKGQSQT RVNINAALVEDIINLEEVNEEMKSVIEALKDNFNKTLNIRTSPGSLDKIAVVTADGKLALNQISQISMKS PQLILVNMASFPECTAAAIKAIRESGMNLNPEVEGTLIRVPIPQSAKWPMTQWQNWTGIWQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_199177 |
ORF Size | 603 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_199177.1, NM_199177.2, NM_199177.3, NP_954646.1 |
RefSeq Size | 1540 bp |
RefSeq ORF | 606 bp |
Locus ID | 92399 |
Protein Families | Transmembrane |
MW | 22.5 kDa |
Gene Summary | This gene encodes a ribosome recycling factor, which is a component of the mitochondrial translational machinery. The encoded protein, along with mitochondrial elongation factor 2, functions in ribosomal recycling at the termination of mitochondrial translation by mediating the disassembly of ribosomes from messenger RNA. A pseudogene of this gene has been identified on chromosome X. [provided by RefSeq, Oct 2016] |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC322027 | MRRF (untagged)-Human mitochondrial ribosome recycling factor (MRRF), nuclear gene encoding mitochondrial protein, transcript variant 2 |
USD 420.00 |
|
RG208798 | MRRF (GFP-tagged) - Human mitochondrial ribosome recycling factor (MRRF), nuclear gene encoding mitochondrial protein, transcript variant 2 |
USD 460.00 |
|
RC208798L3 | Lenti ORF clone of Human mitochondrial ribosome recycling factor (MRRF), nuclear gene encoding mitochondrial protein, transcript variant 2, Myc-DDK-tagged |
USD 620.00 |
|
RC208798L4 | Lenti ORF clone of Human mitochondrial ribosome recycling factor (MRRF), nuclear gene encoding mitochondrial protein, transcript variant 2, mGFP tagged |
USD 620.00 |
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