ARHGAP30 (NM_181720) Human Tagged ORF Clone

CAT#: RC208825

ARHGAP30 (Myc-DDK-tagged)-Human Rho GTPase activating protein 30 (ARHGAP30), transcript variant 2

Product Images

Western blot validation of overexpression lysate (Cat# LY405635) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC208825 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ARHGAP30
• Synonyms: RP11-544M22.6
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC208825 representing NM_181720
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  CTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCCGGCGC
  GCCC
  
  ATGAAGTCTCGGCAGAAAGGAAAGAAGAAGGGCAGCGCAAAGGAGCGGGTTTTTGGGTGCGACTTGCAGG
  AGCACCTGCAGCACTCAGGCCAGGAGGTGCCCCAGGTGCTAAAGAGCTGTGCAGAATTTGTGGAGGAGTA
  TGGAGTGGTGGATGGGATCTACCGCCTCTCAGGGGTCTCCTCCAACATCCAGAAGCTTCGGCAGGAATTG
  GAGTCAGAGCGGAAGCCAGACCTGCGTCGGGATGTTTACCTCCAAGACATTCACTGCGTCTCCTCCCTGT
  GCAAGGCCTATTTCAGAGAACTGCCGGATCCCCTGCTCACTTACCGGCTCTATGACAAGTTTGCTGAGGC
  TGTAGGAGTGCAATTGGAACCTGAGCGCTTGGTCAAGATCCTAGAGGTGCTTCGGGAACTCCCTGTCCCA
  AACTACAGGACCCTGGAGTTCCTCATGAGGCACTTGGTACACATGGCCTCATTCAGTGCCCAGACCAACA
  TGCATGCTCGCAACCTGGCCATCGTGTGGGCTCCCAACCTGCTGAGGTCTAAGGACATAGAGGCCTCAGG
  CTTCAATGGGACAGCGGCCTTCATGGAGGTGCGGGTACAATCCATCGTCGTGGAGTTCATCCTCACACAC
  GTGGACCAGCTCTTTGGGGGTGCTGCCCTCTCTGGTGGTGAGGTGGAGAGTGGGTGGCGATCGCTTCCAG
  GGACCCGGGCATCAGGCAGCCCCGAGGACCTTATGCCCAGGCCACTGCCTTATCACCTGCCTAGCATACT
  GCAGGCTGGCGATGGACCCCCACAGATGCGGCCCTACCATACTATCATCGAGATTGCAGAGCACAAGAGG
  AAGGGGTCTTTGAAGGTCAGGAAGTGGAGGTCTATCTTCAATTTAGGTCGCTCTGGCCATGAGACTAAGC
  GTAAACTTCCACGGGGGGCTGAGGACAGGGAGGATAAATCCAACAAGGGGACACTGCGGCCAGCCAAAAG
  CATGGACTCACTGAGTGCTGCAGCTGGGGCCAGTGATGAGCCAGAGGGGCTGGTGGGGCCCAGCAGCCCC
  CGGCCAAGCCCATTGCTGCCTGAGAGCTTGGAGAACGATTCTATAGAGGCAGCAGAGGGTGAACAGGAGC
  CTGAGGCAGAAGCACTGGGTGGCACAAACTCTGAACCAGGCACACCACGAGCTGGGCGGTCAGCCATCCG
  GGCTGGGGGCAGCAGCCGTGCAGAACGCTGTGCTGGTGTCCACATCTCAGACCCCTACAATGTCAACCTC
  CCGCTACACATCACCTCTATCCTCAGTGTGCCCCCGAACATCATCTCTAACGTTTCCTTGGCCAGGCTCA
  CCCGTGGCCTTGAGTGCCCTGCTCTACAGCACCGGCCAAGCCCTGCCTCTGGCCCTGGCCCTGGCCCTGG
  CCTTGGCCCTGGCCCCCCAGATGAAAAGTTGGAAGCAAGTCCAGCCTCAAGTCCCCTGGCAGACTCAGGC
  CCAGACGACTTGGCTCCTGCCCTGGAGGACTCGCTGTCCCAGGAGGTGCAGGACTCCTTCTCCTTCCTAG
  AGGACTCAAGCAGCTCAGAACCTGAGTGGGTGGGGGCAGAGGATGGGGAGGTGGCCCAGGCAGAAGCAGC
  AGGAGCAGCCTTCTCCCCTGGGGAGGACGACCCTGGGATGGGCTACCTGGAGGAGCTCCTGGGAGTTGGG
  CCTCAGGTGGAGGAGTTCTCTGTGGAGCCACCCCTGGATGACCTGTCTCTGGATGAGGCACAGTTTGTCT
  TGGCCCCCAGCTGCTGTTCCGTGGACTCCGCTGGCCCCAGGCCTGAAGTTGAGGAGGAAAATGGGGAGGA
  AGTTTTCCTGAGTGCCTATGATGACCTAAGTCCCCTTCTGGGACCTAAACCCCCAATCTGGAAGGGTTCA
  GGGAGTCTGGAGGGAGAGGCAGCAGGATGTGGAAGGCAGGCTCTGGGACAGGGTGGGGAAGAGCAGGCAT
  GCTGGGAAGTTGGGGAGGACAAGCAGGCTGAGCCTGGAGGCAGGCTAGACATCAGGGAAGAGGCAGAGGG
  AAGTCCACAGCCACCTCAGCCAGAGGAGATGGAGCCTGAGGGGCAGCCCAGTCCAGACGGCTGTCTATGC
  CCCTGTTCTCTTGGCCTGGGTGGCGTGGGCATGCGTCTAGCTTCCACTCTGGTTCAGGTCCAACAGGTCC
  GCTCTGTGCCTGTGGTGCCCCCCAAGCCACAGTTTGCCAAGATGCCCAGTGCAATGTGTAGCAAGATTCA
  TGTGGCACCTGCAAATCCATGCCCGAGGCCTGGCCGGCTTGATGGGACTCCTGGAGAAAGGGCTTGGGGG
  TCCCGAGCTTCTCGATCCTCTTGGAGGAATGGGGGTAGTCTTTCCTTTGATGCTGCTGTGGCCCTAGCCC
  GGGACCGCCAAAGGACTGAGGCTCAAGGAGTTCGGCGAACCCAGACCTGTACTGAGGGTGGGGATTACTG
  CCTCATCCCCAGAACCTCCCCTTGTAGCATGATCTCTGCCCATTCTCCTCGGCCCCTTAGCTGCCTGGAG
  CTCCCATCTGAAGGTGCAGAAGGGTCTGGATCCCGGAGTCGTCTTAGTCTGCCCCCCAGAGAACCCCAGG
  TTCCTGACCCCCTGTTGTCCTCTCAGCGCAGATCATATGCATTTGAAACACAGGCTAACCCTGGGAAAGG
  TGAAGGACTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC208825 representing NM_181720
  Red=Cloning site Green=Tags(s)
  
  MKSRQKGKKKGSAKERVFGCDLQEHLQHSGQEVPQVLKSCAEFVEEYGVVDGIYRLSGVSSNIQKLRQEL
  ESERKPDLRRDVYLQDIHCVSSLCKAYFRELPDPLLTYRLYDKFAEAVGVQLEPERLVKILEVLRELPVP
  NYRTLEFLMRHLVHMASFSAQTNMHARNLAIVWAPNLLRSKDIEASGFNGTAAFMEVRVQSIVVEFILTH
  VDQLFGGAALSGGEVESGWRSLPGTRASGSPEDLMPRPLPYHLPSILQAGDGPPQMRPYHTIIEIAEHKR
  KGSLKVRKWRSIFNLGRSGHETKRKLPRGAEDREDKSNKGTLRPAKSMDSLSAAAGASDEPEGLVGPSSP
  RPSPLLPESLENDSIEAAEGEQEPEAEALGGTNSEPGTPRAGRSAIRAGGSSRAERCAGVHISDPYNVNL
  PLHITSILSVPPNIISNVSLARLTRGLECPALQHRPSPASGPGPGPGLGPGPPDEKLEASPASSPLADSG
  PDDLAPALEDSLSQEVQDSFSFLEDSSSSEPEWVGAEDGEVAQAEAAGAAFSPGEDDPGMGYLEELLGVG
  PQVEEFSVEPPLDDLSLDEAQFVLAPSCCSVDSAGPRPEVEEENGEEVFLSAYDDLSPLLGPKPPIWKGS
  GSLEGEAAGCGRQALGQGGEEQACWEVGEDKQAEPGGRLDIREEAEGSPQPPQPEEMEPEGQPSPDGCLC
  PCSLGLGGVGMRLASTLVQVQQVRSVPVVPPKPQFAKMPSAMCSKIHVAPANPCPRPGRLDGTPGERAWG
  SRASRSSWRNGGSLSFDAAVALARDRQRTEAQGVRRTQTCTEGGDYCLIPRTSPCSMISAHSPRPLSCLE
  LPSEGAEGSGSRSRLSLPPREPQVPDPLLSSQRRSYAFETQANPGKGEGL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: AscI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_181720
• ORF Size: 2670 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_181720.1, NM_181720.2, NP_859071.2
• RefSeq Size: 3888
• RefSeq ORF: 2673
• Locus ID: 257106
• MW: 95.3 kDa