CD13 (ANPEP) (NM_001150) Human Tagged ORF Clone

CAT#: RC208950

ANPEP (Myc-DDK-tagged)-Human alanyl (membrane) aminopeptidase (ANPEP)

Product Images

Coomassie blue staining of purified ANPEP protein (Cat# TP308950). The protein was produced from HEK293T cells transfected with ANPEP cDNA clone (Cat# RC208950) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ANPEP
• Synonyms: APN; CD13; GP150; LAP1; P150; PEPN
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC208950 representing NM_001150
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCAAGGGCTTCTATATTTCCAAGTCCCTGGGCATCCTGGGGATCCTCCTGGGCGTGGCAGCCGTGT
  GCACAATCATCGCACTGTCAGTGGTGTACTCCCAGGAGAAGAACAAGAACGCCAACAGCTCCCCCGTGGC
  CTCCACCACCCCGTCCGCCTCAGCCACCACCAACCCCGCCTCGGCCACCACCTTGGACCAAAGTAAAGCG
  TGGAATCGTTACCGCCTCCCCAACACGCTGAAACCCGATTCCTACCAGGTGACGCTGAGACCGTACCTCA
  CCCCCAATGACAGGGGCCTGTACGTTTTTAAGGGCTCCAGCACCGTCCGTTTCACCTGCAAGGAGGCCAC
  TGACGTCATCATCATCCACAGCAAGAAGCTCAACTACACCCTCAGCCAGGGGCACAGGGTGGTCCTGCGT
  GGTGTGGGAGGCTCCCAGCCCCCCGACATTGACAAGACTGAGCTGGTGGAGCCCACCGAGTACCTGGTGG
  TGCACCTCAAGGGCTCCCTGGTGAAGGACAGCCAGTATGAGATGGACAGCGAGTTCGAGGGGGAGTTGGC
  AGATGACCTGGCGGGCTTCTACCGCAGCGAGTACATGGAGGGCAATGTCAGAAAGGTGGTGGCCACTACA
  CAGATGCAGGCTGCAGATGCCCGGAAGTCCTTCCCATGCTTCGATGAGCCGGCCATGAAGGCCGAGTTCA
  ACATCACGCTTATCCACCCCAAGGACCTGACAGCCCTGTCCAACATGCTTCCCAAAGGTCCCAGCACCCC
  ACTTCCAGAAGACCCCAACTGGAATGTCACTGAGTTCCACACCACGCCCAAGATGTCCACGTACTTGCTG
  GCCTTCATTGTCAGTGAGTTCGACTACGTGGAGAAGCAGGCATCCAATGGTGTCTTGATCCGGATCTGGG
  CCCGGCCCAGTGCCATTGCGGCGGGCCACGGCGATTATGCCCTGAACGTGACGGGCCCCATCCTTAACTT
  CTTTGCTGGTCATTATGACACACCCTACCCACTCCCAAAATCAGACCAGATTGGCCTGCCAGACTTCAAC
  GCCGGCGCCATGGAGAACTGGGGACTGGTGACCTACCGGGAGAACTCCCTGCTGTTCGACCCCCTGTCCT
  CCTCCAGCAGCAACAAGGAGCGGGTGGTCACTGTGATTGCTCATGAGCTGGCCCACCAGTGGTTCGGGAA
  CCTGGTGACCATAGAGTGGTGGAATGACCTGTGGCTGAACGAGGGCTTCGCCTCCTACGTGGAGTACCTG
  GGTGCTGACTATGCGGAGCCCACCTGGAACTTGAAAGACCTCATGGTGCTGAATGATGTGTACCGCGTGA
  TGGCAGTGGATGCACTGGCCTCCTCCCACCCGCTGTCCACACCCGCCTCGGAGATCAACACGCCGGCCCA
  GATCAGTGAGCTGTTTGACGCCATCTCCTACAGCAAGGGCGCCTCAGTCCTCAGGATGCTCTCCAGCTTC
  CTGTCCGAGGACGTATTCAAGCAGGGCCTGGCGTCCTACCTCCACACCTTTGCCTACCAGAACACCATCT
  ACCTGAACCTGTGGGACCACCTGCAGGAGGCTGTGAACAACCGGTCCATCCAACTCCCCACCACCGTGCG
  GGACATCATGAACCGCTGGACCCTGCAGATGGGCTTCCCGGTCATCACGGTGGATACCAGCACGGGGACC
  CTTTCCCAGGAGCACTTCCTCCTTGACCCCGATTCCAATGTTACCCGCCCCTCAGAATTCAACTACGTGT
  GGATTGTGCCCATCACATCCATCAGAGATGGCAGACAGCAGCAGGACTACTGGCTGATAGATGTAAGAGC
  CCAGAACGATCTCTTCAGCACATCAGGCAATGAGTGGGTCCTGCTGAACCTCAATGTGACGGGCTATTAC
  CGGGTGAACTACGACGAAGAGAACTGGAGGAAGATTCAGACTCAGCTGCAGAGAGACCACTCGGCCATCC
  CTGTCATCAATCGGGCACAGATCATTAATGACGCCTTCAACCTGGCCAGTGCCCATAAGGTCCCTGTCAC
  TCTGGCGCTGAACAACACCCTCTTCCTGATTGAAGAGAGACAGTACATGCCCTGGGAGGCCGCCCTGAGC
  AGCCTGAGCTACTTCAAGCTCATGTTTGACCGCTCCGAGGTCTATGGCCCCATGAAGAACTACCTGAAGA
  AGCAGGTCACACCCCTCTTCATTCACTTCAGAAATAATACCAACAACTGGAGGGAGATCCCAGAAAACCT
  GATGGACCAGTACAGCGAGGTTAATGCCATCAGCACCGCCTGCTCCAACGGAGTTCCAGAGTGTGAGGAG
  ATGGTCTCTGGCCTTTTCAAGCAGTGGATGGAGAACCCCAATAATAACCCGATCCACCCCAACCTGCGGT
  CCACCGTCTACTGCAACGCTATCGCCCAGGGCGGGGAGGAGGAGTGGGACTTCGCCTGGGAGCAGTTCCG
  AAATGCCACACTGGTCAATGAGGCTGACAAGCTCCGGGCAGCCCTGGCCTGCAGCAAAGAGTTGTGGATC
  CTGAACAGGTACCTGAGCTACACCCTGAACCCGGACTTAATCCGGAAGCAGGACGCCACCTCTACCATCA
  TCAGCATTACCAACAACGTCATTGGGCAAGGTCTGGTCTGGGACTTTGTCCAGAGCAACTGGAAGAAGCT
  TTTTAACGATTATGGTGGTGGCTCGTTCTCCTTCTCCAACCTCATCCAGGCAGTGACACGACGATTCTCC
  ACCGAGTATGAGCTGCAGCAGCTGGAGCAGTTCAAGAAGGACAACGAGGAAACAGGCTTCGGCTCAGGCA
  CCCGGGCCCTGGAGCAAGCCCTGGAGAAGACGAAAGCCAACATCAAGTGGGTGAAGGAGAACAAGGAGGT
  GGTGCTCCAGTGGTTCACAGAAAACAGCAAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC208950 representing NM_001150
  Red=Cloning site Green=Tags(s)
  
  MAKGFYISKSLGILGILLGVAAVCTIIALSVVYSQEKNKNANSSPVASTTPSASATTNPASATTLDQSKA
  WNRYRLPNTLKPDSYQVTLRPYLTPNDRGLYVFKGSSTVRFTCKEATDVIIIHSKKLNYTLSQGHRVVLR
  GVGGSQPPDIDKTELVEPTEYLVVHLKGSLVKDSQYEMDSEFEGELADDLAGFYRSEYMEGNVRKVVATT
  QMQAADARKSFPCFDEPAMKAEFNITLIHPKDLTALSNMLPKGPSTPLPEDPNWNVTEFHTTPKMSTYLL
  AFIVSEFDYVEKQASNGVLIRIWARPSAIAAGHGDYALNVTGPILNFFAGHYDTPYPLPKSDQIGLPDFN
  AGAMENWGLVTYRENSLLFDPLSSSSSNKERVVTVIAHELAHQWFGNLVTIEWWNDLWLNEGFASYVEYL
  GADYAEPTWNLKDLMVLNDVYRVMAVDALASSHPLSTPASEINTPAQISELFDAISYSKGASVLRMLSSF
  LSEDVFKQGLASYLHTFAYQNTIYLNLWDHLQEAVNNRSIQLPTTVRDIMNRWTLQMGFPVITVDTSTGT
  LSQEHFLLDPDSNVTRPSEFNYVWIVPITSIRDGRQQQDYWLIDVRAQNDLFSTSGNEWVLLNLNVTGYY
  RVNYDEENWRKIQTQLQRDHSAIPVINRAQIINDAFNLASAHKVPVTLALNNTLFLIEERQYMPWEAALS
  SLSYFKLMFDRSEVYGPMKNYLKKQVTPLFIHFRNNTNNWREIPENLMDQYSEVNAISTACSNGVPECEE
  MVSGLFKQWMENPNNNPIHPNLRSTVYCNAIAQGGEEEWDFAWEQFRNATLVNEADKLRAALACSKELWI
  LNRYLSYTLNPDLIRKQDATSTIISITNNVIGQGLVWDFVQSNWKKLFNDYGGGSFSFSNLIQAVTRRFS
  TEYELQQLEQFKKDNEETGFGSGTRALEQALEKTKANIKWVKENKEVVLQWFTENSK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001150
• ORF Size: 2901 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001150.1, NM_001150.2, NP_001141.1
• RefSeq Size: 3494 bp
• RefSeq ORF: 2904 bp
• Locus ID: 290
• Cytogenetics: 15q26.1
• Domains: Peptidase_M1
• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Protease, Transmembrane
• Protein Pathways: Glutathione metabolism, Hematopoietic cell lineage, Metabolic pathways, Renin-angiotensin system
• MW: 109.51 kDa
• Gene Summary: 'Aminopeptidase N is located in the small-intestinal and renal microvillar membrane, and also in other plasma membranes. In the small intestine aminopeptidase N plays a role in the final digestion of peptides generated from hydrolysis of proteins by gastric and pancreatic proteases. Its function in proximal tubular epithelial cells and other cell types is less clear. The large extracellular carboxyterminal domain contains a pentapeptide consensus sequence characteristic of members of the zinc-binding metalloproteinase superfamily. Sequence comparisons with known enzymes of this class showed that CD13 and aminopeptidase N are identical. The latter enzyme was thought to be involved in the metabolism of regulatory peptides by diverse cell types, including small intestinal and renal tubular epithelial cells, macrophages, granulocytes, and synaptic membranes from the CNS. This membrane-bound zinc metalloprotease is known to serve as a receptor for the HCoV-229E alphacoronavirus as well as other non-human coronaviruses. This gene has also been shown to promote angiogenesis, tumor growth, and metastasis and defects in this gene are associated with various types of leukemia and lymphoma. [provided by RefSeq, Apr 2020]'