CRMP2 (DPYSL2) (NM_001386) Human Tagged ORF Clone

CAT#: RC209080

DPYSL2 (Myc-DDK-tagged)-Human dihydropyrimidinase-like 2 (DPYSL2), transcript variant 2

Product Images

Western blot validation of overexpression lysate (Cat# LY419992) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC209080 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified DPYSL2 protein (Cat# TP309080). The protein was produced from HEK293T cells transfected with DPYSL2 cDNA clone (Cat# RC209080) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: DPYSL2
• Synonyms: CRMP-2; CRMP2; DHPRP2; DRP-2; DRP2; N2A3; ULIP-2; ULIP2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC209080 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTTATCAGGGGAAGAAAAATATTCCACGCATCACGAGCGATCGTCTTCTGATCAAAGGAGGTAAAA
  TTGTTAATGATGACCAGTCGTTCTATGCAGACATATACATGGAAGATGGGTTGATCAAGCAAATAGGAGA
  AAATCTGATTGTGCCAGGAGGAGTGAAGACCATCGAGGCCCACTCCCGGATGGTGATCCCCGGAGGAATT
  GACGTCCACACTCGTTTCCAGATGCCTGATCAGGGAATGACGTCTGCTGATGATTTCTTCCAAGGAACCA
  AGGCGGCCCTGGCTGGGGGAACCACTATGATCATTGACCACGTTGTTCCTGAGCCTGGGACAAGCCTGCT
  CGCTGCCTTTGACCAGTGGAGGGAATGGGCCGACAGCAAGTCCTGCTGTGACTACTCTCTGCATGTGGAC
  ATCAGCGAGTGGCATAAGGGCATCCAGGAGGAGATGGAAGCGCTTGTGAAGGATCACGGGGTAAATTCCT
  TCCTCGTGTACATGGCTTTCAAAGATCGCTTCCAGCTAACGGATTGCCAGATTTATGAAGTACTGAGTGT
  GATCCGGGATATTGGCGCCATAGCCCAAGTCCACGCAGAAAATGGCGACATCATTGCAGAGGAGCAGCAG
  AGGATCCTGGATCTGGGCATCACGGGCCCCGAGGGACATGTGCTGAGCCGACCTGAGGAGGTCGAGGCCG
  AAGCCGTGAATCGTGCCATCACCATCGCCAACCAGACCAACTGCCCGCTGTATATCACCAAGGTGATGAG
  CAAAAGCTCTGCTGAGGTCATCGCCCAGGCACGGAAGAAGGGAACTGTGGTGTATGGCGAGCCCATCACT
  GCCAGCTTGGGAACGGACGGCTCCCATTACTGGAGCAAGAACTGGGCCAAGGCTGCTGCCTTTGTCACCT
  CCCCACCCTTGAGCCCTGATCCAACCACTCCAGACTTTCTCAACTCCTTGCTGTCCTGTGGAGACCTCCA
  GGTCACGGGCAGTGCCCATTGCACGTTTAACACTGCCCAGAAGGCTGTAGGAAAGGACAACTTCACCCTG
  ATTCCGGAGGGCACCAATGGCACTGAGGAGCGGATGTCCGTCATCTGGGACAAGGCTGTGGTCACTGGGA
  AGATGGATGAGAACCAGTTTGTGGCTGTGACCAGCACCAATGCAGCCAAAGTCTTCAACCTTTACCCCCG
  GAAAGGCCGCATTGCTGTGGGATCCGATGCCGACCTGGTCATCTGGGACCCCGACAGCGTTAAAACCATC
  TCTGCCAAGACACACAACAGCTCTCTCGAGTACAACATCTTTGAAGGCATGGAGTGCCGCGGCTCCCCAC
  TGGTAGTCATCAGCCAGGGGAAGATTGTCCTGGAGGACGGCACCCTGCATGTCACCGAAGGCTCTGGACG
  CTACATTCCCCGGAAGCCCTTCCCTGATTTTGTTTACAAGCGTATCAAGGCAAGGAGCAGGCTGGCTGAG
  CTGAGAGGGGTTCCTCGTGGCCTGTATGACGGACCCGTGTGTGAAGTGTCTGTGACGCCCAAGACAGTCA
  CTCCAGCCTCCTCGGCCAAGACGTCTCCTGCCAAGCAGCAGGCCCCACCTGTCCGGAACCTGCACCAGTC
  TGGATTCAGTTTGTCTGGTGCTCAGATTGATGACAACATTCCCCGCCGCACCACCCAGCGTATCGTGGCG
  CCCCCCGGTGGCCGTGCCAACATCACCAGCCTGGGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC209080 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSYQGKKNIPRITSDRLLIKGGKIVNDDQSFYADIYMEDGLIKQIGENLIVPGGVKTIEAHSRMVIPGGI
  DVHTRFQMPDQGMTSADDFFQGTKAALAGGTTMIIDHVVPEPGTSLLAAFDQWREWADSKSCCDYSLHVD
  ISEWHKGIQEEMEALVKDHGVNSFLVYMAFKDRFQLTDCQIYEVLSVIRDIGAIAQVHAENGDIIAEEQQ
  RILDLGITGPEGHVLSRPEEVEAEAVNRAITIANQTNCPLYITKVMSKSSAEVIAQARKKGTVVYGEPIT
  ASLGTDGSHYWSKNWAKAAAFVTSPPLSPDPTTPDFLNSLLSCGDLQVTGSAHCTFNTAQKAVGKDNFTL
  IPEGTNGTEERMSVIWDKAVVTGKMDENQFVAVTSTNAAKVFNLYPRKGRIAVGSDADLVIWDPDSVKTI
  SAKTHNSSLEYNIFEGMECRGSPLVVISQGKIVLEDGTLHVTEGSGRYIPRKPFPDFVYKRIKARSRLAE
  LRGVPRGLYDGPVCEVSVTPKTVTPASSAKTSPAKQQAPPVRNLHQSGFSLSGAQIDDNIPRRTTQRIVA
  PPGGRANITSLG
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001386
• ORF Size: 1716 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001386.1, NM_001386.2, NM_001386.3, NM_001386.4, NM_001386.5, NP_001377.1
• RefSeq Size: 4638 bp
• RefSeq ORF: 1719 bp
• Locus ID: 1808
• Cytogenetics: 8p21.2
• Domains: Amidohydro_1
• Protein Families: Druggable Genome
• Protein Pathways: Axon guidance
• MW: 62.3 kDa
• Gene Summary: 'This gene encodes a member of the collapsin response mediator protein family. Collapsin response mediator proteins form homo- and hetero-tetramers and facilitate neuron guidance, growth and polarity. The encoded protein promotes microtubule assembly and is required for Sema3A-mediated growth cone collapse, and also plays a role in synaptic signaling through interactions with calcium channels. This gene has been implicated in multiple neurological disorders, and hyperphosphorylation of the encoded protein may play a key role in the development of Alzheimer's disease. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Sep 2011]'